Experience with subzonal insemination (SUZI) and intracytoplasmic sperm injection (ICSI) on unfertilized aged human oocytes

1994 ◽  
Vol 11 (8) ◽  
pp. 389-394 ◽  
Author(s):  
Marinos Tsirigotis ◽  
Valerie Bennett ◽  
Natasha Nicholson ◽  
Yasser Khalifa ◽  
Georg Hogewind ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Human Oocytes ◽  
Subzonal Insemination

Higher success rate by intracytoplasmic sperm injection than by subzonal insemination. Report of a second series of 300 consecutive treatment cycles

1993 ◽  
Vol 8 (7) ◽  
pp. 1055-1060 ◽  
Author(s):  
André C. Van Steirteghem ◽  
Jiaen Liu ◽  
Hubert Joris ◽  
Zsolt Nagy ◽  
Cécile Janssenswillen ◽  
...  
Keyword(s):  
Success Rate ◽  
Intracytoplasmic Sperm Injection ◽  
Subzonal Insemination

Development and implementation of intracytoplasmic sperm injection (ICSI)

1995 ◽  
Vol 7 (2) ◽  
pp. 211 ◽  
Author(s):  
GD Palermo ◽  
J Cohen ◽  
M Alikani ◽  
A Adler ◽  
Z Rosenwaks
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Human Sperm ◽  
Fertilization Rate ◽  
Semen Parameters ◽  
Ongoing Pregnancy Rate ◽  
Vitro Fertilization ◽  
Human Oocytes ◽  
Intracytoplasmic Injection ◽  
Previously Treated

The purpose of this paper is to elucidate the experimental steps that led to the development of intracytoplasmic sperm injection (ICSI) and its application in the human. ICSI has become the most successful micromanipulation procedure for treating male infertility. A total of 355 in vitro fertilization (IVF) cycles utilizing ICSI are described; 180 couples were previously treated in 509 IVF cycles but achieved no fertilization and 175 couples could not be treated by IVF because of extremely poor semen parameters. Of the 3063 metaphase II (M II) oocytes retrieved, 2970 were injected with a survival rate of 93.6%, yielding 1917 bipronuclear zygotes (64.5%). In 148 patients, a foetal heart was evidenced by ultrasound; 11 of these patients miscarried between 7 and 13 weeks of gestation. The ongoing pregnancy rate was 38.6% (137/355) per retrieval and 40.5% (137/338) per embryo replacement. At the time of writing, there were 22 deliveries and one therapeutic abortion for a trisomy 21 chromosomal abnormality. In addition, 66 singleton, 37 twin, 10 triplet and 1 quadruplet pregnancies were ongoing. The concentration of motile spermatozoa in the ejaculate only slightly influenced the fertilization rate (P < 0.001) and the pregnancy outcome (P < 0.01). A preliminary injection procedure utilizing intracytoplasmic injection of isolated sperm heads was performed in 35 M II human oocytes with resultant fertilization and cleavage rates of 74% and 73% respectively. Skills in ICSI were acquired by injecting hamster and unfertilized human oocytes with human sperm. ICSI can be used to successfully treat couples who have failed IVF or who have too few spermatozoa for conventional in vitro insemination.(ABSTRACT TRUNCATED AT 250 WORDS)

Assisted fertilization by subzonal insemination and intracytoplasmic sperm injection

1994 ◽  
Vol 6 (1) ◽  
pp. 85 ◽  
Author(s):  
Steirteghem A Van ◽  
J Liu ◽  
H Joris ◽  
Z Nagy ◽  
C Staessen ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Congenital Malformations ◽  
Subsequent Development ◽  
Fertilization Rate ◽  
Subzonal Insemination ◽  
Failed Fertilization ◽  
Assisted Fertilization ◽  
Normal Fertilization

The results of 600 consecutive treatment cycles of subzonal insemination (SUZI) and intracytoplasmic sperm injection (ICSI) are described in couples with failed fertilization after standard IVF or insufficient spermatozoa in the ejaculate for IVF. More oocytes were damaged by ICSI (16.3%) than by SUZI (8.5%) and the normal fertilization rate was substantially higher after ICSI (49.1% v. 16.6%). Subsequent development of two-pronuclear oocytes in vitro was 80% after SUZI and 73.9% after ICSI. Significantly more triple embryo replacements were carried out after ICSI than after SUZI. Embryo transfers were possible in 421 of the 600 cycles. There were 63 pregnancies after ICSI (215 transfers) and 23 after SUZI (156 transfers); 10 additional pregnancies were achieved after 50 transfers of a mixture of SUZI and ICSI embryos. The results of fetal karyotypes and follow-up of the children do not indicate an increase in congenital malformations.

Intracytoplasmic sperm injection in dysmorphic human oocytes

Zygote ◽  
1995 ◽  
Vol 3 (4) ◽  
pp. 283-288 ◽  
Author(s):  
Mina Alikani ◽  
Gianpiero Palermo ◽  
Alexis Adler ◽  
Massimo Bertoil ◽  
Marlena Blake ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Smooth Endoplasmic Reticulum ◽  
Polar Body ◽  
Light Microscopic Level ◽  
Human Oocytes ◽  
Fertilisation Rate ◽  
Polar Bodies ◽  
First Polar Body ◽  
Multiple Abnormalities ◽  
Further Development

SummaryFertilisation and development of dysmorphic human oocytes recovered from hyperstimulated ovaries have been evaluated following intracytoplasmic sperm injection (ICSI) for treatment of male infertility. A total of 2968 oocytes at metaphase II of meiosis were injected, of which 806 (27.2%) were dysmorphic at the light microscopic level. Cytoplasmic abnormalities included granularity, areas of necrosis, organelle clustering, vacuoles, and accumulating saccules of smooth endoplasmic reticulum. Anomalies of the first polar body and zona pellucida, as well as non-spherical shapes of oocytes, were also noted. Contrary to previous findings linking some dysmorphisms to non-assisted fertilisation failure, in this study no single abnormality led to a reduction in the fertilisation rate, nor was fertilisation compromised in oocytes with multiple abnormalities. The incidence of normal fertilisation (two pronuclei and two polar bodies) was 69% in both the dysmorphic and non-dysmorphic oocytes. While overall pregnancy and implantation results were not altered in the group of patients (n = 242) in whom at least one dysmorphic oocyte was injected, exclusive replacement of embryos which originated from dysmorphic oocytes led to a higher incidence of early pregnancy loss. It is concluded that aberrations in the morphology of human oocytes – most probably a product of controlled ovarian stimulation – are of little or no consequence to fertilisation or early cleavage after ICSI. It is possible, however, that these embryos have a reduced potential for implantation and further development.

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