Crystal structure of pokeweed antiviral protein from seeds ofPhytolacca americana at 0.25 nm

1998 ◽  
Vol 41 (4) ◽  
pp. 413-418
Author(s):  
Zonghao Zeng ◽  
Lei Jin ◽  
Hongmin Li ◽  
Zhong Hu ◽  
Dacheng Wang
Keyword(s):  
Crystal Structure ◽  
Pokeweed Antiviral Protein ◽  
Antiviral Protein

Crystal structure of pokeweed antiviral protein with well-defined sugars from seeds at 1.8Å resolution

2003 ◽  
Vol 141 (2) ◽  
pp. 171-178 ◽  
Author(s):  
Zong-Hao Zeng ◽  
Xiao-Lin He ◽  
Hong-Min Li ◽  
Zhong Hu ◽  
Da-Cheng Wang
Keyword(s):  
Crystal Structure ◽  
Pokeweed Antiviral Protein ◽  
Antiviral Protein

Development of a double sandwich ELISA able to discriminate between free PAP (pokeweed antiviral protein) and complexed PAP in leaf extracts

Plant Science ◽  
1999 ◽  
Vol 140 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Jean-Luc Schlick ◽  
Bénédicte Desvoyes ◽  
Mehdi Hakil ◽  
Pascale Adami ◽  
Philippe Dulieu
Keyword(s):  
Sandwich Elisa ◽  
Pokeweed Antiviral Protein ◽  
Antiviral Protein ◽  
Leaf Extracts

The 2·5 Å Structure of Pokeweed Antiviral Protein

1993 ◽  
Vol 233 (4) ◽  
pp. 705-715 ◽  
Author(s):  
Arthur F. Monzingo ◽  
Edward J. Collins ◽  
Stephen R. Ernst ◽  
James D. Irvin ◽  
Jon D. Robertus
Keyword(s):  
Pokeweed Antiviral Protein ◽  
Antiviral Protein

scholarly journals Expression of a Truncated Form of Ribosomal Protein L3 Confers Resistance to Pokeweed Antiviral Protein and the Fusarium Mycotoxin Deoxynivalenol

2005 ◽  
Vol 18 (8) ◽  
pp. 762-770 ◽  
Author(s):  
Rong Di ◽  
Nilgun E. Tumer
Keyword(s):  
Ribosomal Protein ◽  
Fusarium Species ◽  
Common Mechanism ◽  
Pokeweed Antiviral Protein ◽  
Wild Type ◽  
Antiviral Protein ◽  
Trichothecene Mycotoxin ◽  
Truncated Form ◽  
Fusarium Mycotoxin ◽  
Ribosomal Protein L3

The contamination of important agricultural products such as wheat, barley, or maize with the trichothecene mycotoxin deoxynivalenol (DON) due to infection with Fusarium species is a worldwide problem. Trichothecenes inhibit protein synthesis by targeting ribosomal protein L3. Pokeweed antiviral protein (PAP), a ribosome-inactivating protein binds to L3 to depurinate the α–sarcin/loop of the large rRNA. Plants transformed with the wild-type PAP show lesions and express very low levels of PAP because PAP autoregulates its expression by destabilizing its own mRNA. We show here that transgenic tobacco plants expressing both the wild-type PAP and a truncated form of yeast L3 (L3δ) are phenotypically normal. PAP mRNA and protein levels are very high in these plants, indicating that L3δ suppresses the autoregulation of PAP mRNA expression. Ribosomes are not depurinated in the transgenic plants expressing PAP and L3δ, even though PAP is associated with ribosomes. The expression of the endogenous tobacco ribosomal protein L3 is up-regulated in these plants and they are resistant to the Fusarium mycotoxin DON. These results demonstrate that expression of an N-terminal fragment of yeast L3 leads to trans-dominant resistance to PAP and the trichothecene mycotoxin DON, providing evidence that both toxins target L3 by a common mechanism.

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