Identification of late blight, Colorado potato beetle, and blackleg resistance in three Mexican and two South American wild 2x (1EBN)Solanum species

2003 ◽  
Vol 80 (1) ◽  
pp. 9-19 ◽  
Author(s):  
Q. Chen ◽  
L. M. Kawchuk ◽  
D. R. Lynch ◽  
M. S. Goettel ◽  
D. K. Fujimoto
Keyword(s):  
Late Blight ◽  
Colorado Potato Beetle ◽  
Solanum Species ◽  
South American ◽  
Blackleg Resistance ◽  
Potato Beetle

scholarly journals Irish Potato, Control of Colorado Potato Beetle, 1994

1995 ◽  
Vol 20 (1) ◽  
pp. 119-119
Author(s):  
Gary H. Sewell ◽  
Richard H. Storch
Keyword(s):  
Late Blight ◽  
Colorado Potato Beetle ◽  
Bare Soil ◽  
Irish Potato ◽  
Block Designs ◽  
Average Temperature ◽  
Potato Beetle ◽  
Whole Seed ◽  
Whole Plants ◽  
Presque Isle

Abstract The experiments were randomized complete block designs with six replicates each. The four experiments were planted 23 and 24 May at Presque Isle, ME on land that was in clover in 1993. The 3.0 m space between blocks and the 1.8 m space between the four-row plots were bare soil. All rows were planted with an assisted-feed planter 0.9 m apart and 15.0 m long and the healthy whole seed (ca. 75 g) were 30 cm apart. Fertilization and hilling practices were normal for the area. Early and late blight were controlled by eight foliar applications of chlorothalonil. Foliar insecticide applications were made with a Century boom sprayer, three nozzles per row at 6.3 kg/cm2 pressure and 0.94 k liter/ha. Numbers of larvae and adults were counted weekly on 25 whole plants in the middle rows of each plot. When dates of count and application were the same, beetle counts were made prior to application of insecticides. Analyses of variance were calculated following transformations of data using log10(x + 1). Transformed means of the sum of larvae and adult beetles were compared using Fisher’s LSD Test and then applied to the actual means. The soil was classified as Caribou Loam, pH 5.2. Total monthly rainfall for Jun, Jul, and Aug was respectively 10.9, 9.6, and 3.3 cm. The average temperature for each of the same 3 months was 17.9, 20.3 and 17.7°C.

Development of an effective protoplast fusion system for production of new potatoes with disease and insect resistance using Mexican wild potato species as gene pools

2008 ◽  
Vol 88 (4) ◽  
pp. 611-619 ◽  
Author(s):  
Qin Chen ◽  
H. Y. Li ◽  
Y. Z. Shi ◽  
D. Beasley ◽  
B. Bizimungu ◽  
...  
Keyword(s):  
Late Blight ◽  
Protoplast Fusion ◽  
Insect Resistance ◽  
Colorado Potato Beetle ◽  
Wild Species ◽  
Somatic Hybrids ◽  
Fusion System ◽  
Cultivated Potato ◽  
Potato Species ◽  
Potato Beetle

Somatic hybridization through protoplast fusion is an important alternative approach for overcoming sexual incompatibility between diploid Solanum species and cultivated potatoes. However, compared with other potato species, methods for protoplast isolation and plant generation for several Mexican wild diploid potato species are not well established. In this study, a systematic procedure was designed for the isolation of a large number of high-quality protoplasts from various Mexican wild species that carry high levels of disease (late blight) and insect [Colorado potato beetle (CPB)] resistance. Using this procedure, an effective potato protoplast fusion system was developed to produce new somatic hybrids between two Mexican, one Argentina wild species, and cultivated potato clones. Regenerated plants and somatic hybrids were obtained at a high frequency from the protoplasts of the diploid wild species and their fused cells with S. tuberosum. Morphological, cytological and molecular marker analyses demonstrated that somatic hybrids were successfully obtained from the cell fusion of S. tuberosum and the diploid species S. pinnatisectum, S. cardiophyllum, and S. chacoense. Assessment of disease and insect reactions demonstrated that several of the protoplast-derived clones and somatic hybrids showed a higher level of resistance to both late blight and CPB than was found in S. tuberosum, confirming that the protoplast system is a powerful tool in potato breeding programs for the development of disease and insect resistance. This new fusion system provides breeders with opportunities to transfer disease and insect resistance genes from Mexican wild species into cultivated potato. Key words: Somatic hybrid, protoplast, fusion, potato, Solanum, late blight, disease resistance, Colorado potato beetle insect resistance

scholarly journals The usage of biological system for potato protection from Colorado potato beetle (Leptonotarsa decemlineata Say.) and late blight (Phytophthora infestans (Mont) de Bary)

2018 ◽  
pp. 208-218
Author(s):  
M. Solomiychuk ◽  
Yu. Kordulyan
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Colorado Potato Beetle ◽  
Biological System ◽  
Potato Yield ◽  
Biological Products ◽  
Potato Beetle

The results of research and practical use of biological products in different schomes of potato protection are presented from Colorado potato beetle and late blight. The Colorado potato beetle’s season dynamics was observed. The insecticide’s actions efficiency against Colorado potato beetle’s imaho and larva during the sowing spray was shown. The biological fungicides and insecticides combination impact was researched in potato treating complexes against pest and late blight appearing. The biological preparations usage together with preparations of chemical origin for tubers treating was favoured significantly potato yield enlargement and it’s quality increase.

Novel Mechanism for Resistance to Colorado Potato Beetle (Coleoptera: Chrysomelidae) in Wild Solanum Species

1997 ◽  
Vol 90 (2) ◽  
pp. 689-696 ◽  
Author(s):  
Evans Sikinyi ◽  
David J. Hannapel ◽  
Paula M. Imerman ◽  
H. M. Stahr
Keyword(s):  
Colorado Potato Beetle ◽  
Solanum Species ◽  
Wild Solanum Species ◽  
Potato Beetle ◽  
Wild Solanum

MEASURING RESISTANCE TO THE COLORADO POTATO BEETLE (COLEOPTERA: CHRYSOMELIDAE) IN POTATO

1987 ◽  
Vol 22 (3) ◽  
pp. 245-252 ◽  
Author(s):  
William W. Cantelo ◽  
Larry W. Douglass ◽  
Lind L. Sanford ◽  
Stephen L. Sinden ◽  
Kenneth L. Deahl
Keyword(s):  
Colorado Potato Beetle ◽  
Solanum Species ◽  
Sensitive Test ◽  
The Third ◽  
Stage Of Development ◽  
Potato Beetle ◽  
Alpha Level ◽  
Neonate Larvae ◽  
Test Plants ◽  
Leaf Disks

A sensitive test was sought that could be used to detect small differences in resistance to the Colorado potato beetle (CPB) in Solanum species. Three tests were evaluated and compared. One test compared adult CPB foliage consumption of leaf disks from a susceptible potato cv, S. tuberosum, with disks from two S. chacoense clones. The second test compared weight gain on foliage from the three plants by 4th instar larvae and the third test compared larval development rate and mortality. With sufficient replication all three of the tests could detect significant differences between each of the test clones. The most sensitive test measured the stage of development of neonate larvae after feeding on test plants four days. This test required only four replicates to detect a 50% difference from the overall mean assuming an alpha level of 0.05 and a beta level of 0.10.

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