Influence of zinc concentration in nutrient solution on growth and elemental content of the “Katahdin” potato plant

1974 ◽  
Vol 51 (11) ◽  
pp. 345-354 ◽  
Author(s):  
A. R. Langille ◽  
R. I. Batteese
1974 ◽  
Vol 54 (2) ◽  
pp. 375-381 ◽  
Author(s):  
ALAN R. LANGILLE ◽  
R. I. BATTEESE Jr.

Hoagland’s solutions containing nine levels of manganese ranging from 0 to 100 ppm were applied as a drench tri-weekly to potato plants (Solanum tuberosum L. cv. Katahdin) growing in perlite. Although no deficiency symptoms were observed during the 48-day growing period, varying degrees of toxicity were associated with concentrations of 25.0 ppm Mn and higher in nutrient solution. The tissue concentration necessary to induce toxicity symptoms in foliage appeared to fall between 134 and 426 ppm Mn. Solution concentrations in excess of 25 ppm Mn caused a significant reduction in dry weight of top, root and rhizome portions, and in rhizome number. Increasing Mn treatments generally influenced elemental tissue content as follows: K and Mn increased, Mg decreased, Al and Ca decreased at and above 50 ppm and 75 ppm Mn, respectively, whereas P increased at 50 ppm Mn and above; B, Cu and Mo were unaffected and although N, Fe and Zn contents were somewhat erratic, they were not significantly different at 100 ppm Mn from the control (0.50 ppm Mn).


2008 ◽  
Vol 38 (6) ◽  
pp. 1529-1533 ◽  
Author(s):  
Manuel Benito Novella ◽  
Jerônimo Luiz Andriolo ◽  
Dilson Antônio Bisognin ◽  
Clarissa Melo Cogo ◽  
Maurício Guerra Bandinelli

The effect of the nutrient solution concentration on potato plant growth and minituber yield were determined in a sand closed hydroponic system. Minitubers and micropropagated plantlets of the cv. 'Macaca' were used. Treatments were five nutrient solution concentrations at electrical conductivities (EC) of 1.0 (T1), 2.2 (T2), 3.4 (T3), 4.7 (T4) and 5.8dS m-1 (T5). The split plot randomised experimental design was used with three replications. Plants from minitubers produced higher fresh and mean weight of minitubers, shoot dry mass and leaf area index than the micropropagated ones. However, higher dry mass of minitubers was found with micropropagated plantlets compared to minitubers. The concentration of the nutrient solution did not affect minituber number. Increasing the nutrient solution concentration decreased total and minituber dry mass production of micropropagated plantlets and plant growth and minituber production of minituber-originated plants. Low concentration of nutrient solution at an EC of about 1.0dS m-1 can be used in the hydroponic production of potato minitubers of both micropropagated and minituber-originated plants.


2015 ◽  
Vol 393 (1-2) ◽  
pp. 147-162 ◽  
Author(s):  
Emir Ali Ova ◽  
Umit Baris Kutman ◽  
Levent Ozturk ◽  
Ismail Cakmak

Author(s):  
Claude Lechene

Electron probe microanalysis of frozen hydrated kidneysThe goal of the method is to measure on the same preparation the chemical elemental content of the renal luminal tubular fluid and of the surrounding renal tubular cells. The following method has been developed. Rat kidneys are quenched in solid nitrogen. They are trimmed under liquid nitrogen and mounted in a copper holder using a conductive medium. Under liquid nitrogen, a flat surface is exposed by sawing with a diamond saw blade at constant speed and constant pressure using a custom-built cryosaw. Transfer into the electron probe column (Cameca, MBX) is made using a simple transfer device maintaining the sample under liquid nitrogen in an interlock chamber mounted on the electron probe column. After the liquid nitrogen is evaporated by creating a vacuum, the sample is pushed into the special stage of the instrument. The sample is maintained at close to liquid nitrogen temperature by circulation of liquid nitrogen in the special stage.


Author(s):  
N.K.R. Smith ◽  
K.E. Hunter ◽  
P. Mobley ◽  
L.P. Felpel

Electron probe energy dispersive x-ray microanalysis (XRMA) offers a powerful tool for the determination of intracellular elemental content of biological tissue. However, preparation of the tissue specimen , particularly excitable central nervous system (CNS) tissue , for XRMA is rather difficult, as dissection of a sample from the intact organism frequently results in artefacts in elemental distribution. To circumvent the problems inherent in the in vivo preparation, we turned to an in vitro preparation of astrocytes grown in tissue culture. However, preparations of in vitro samples offer a new and unique set of problems. Generally, cultured cells, growing in monolayer, must be harvested by either mechanical or enzymatic procedures, resulting in variable degrees of damage to the cells and compromised intracel1ular elemental distribution. The ultimate objective is to process and analyze unperturbed cells. With the objective of sparing others from some of the same efforts, we are reporting the considerable difficulties we have encountered in attempting to prepare astrocytes for XRMA.Tissue cultures of astrocytes from newborn C57 mice or Sprague Dawley rats were prepared and cultured by standard techniques, usually in T25 flasks, except as noted differently on Cytodex beads or on gelatin. After different preparative procedures, all samples were frozen on brass pins in liquid propane, stored in liquid nitrogen, cryosectioned (0.1 μm), freeze dried, and microanalyzed as previously reported.


2008 ◽  
Vol 70 (4) ◽  
pp. 402-405 ◽  
Author(s):  
Chie HIRABE ◽  
Chisato HOSOKAWA ◽  
Masakazu TAKAHARA ◽  
Satoko SHIBATA ◽  
Satoshi TAKEUCHI ◽  
...  

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