Production of somatic hybrids between frost tolerantSolanum commersonii andS. Tuberosum: Protoplast fusion, regeneration and isozyme analysis

1993 ◽  
Vol 70 (11) ◽  
pp. 753-764 ◽  
Author(s):  
T. Cardi ◽  
K. J. Puite ◽  
K. S. Ramulu ◽  
F. D’Ambrosio ◽  
L. Frusciante
Keyword(s):  
Protoplast Fusion ◽  
Somatic Hybrids ◽  
Isozyme Analysis

Production and characterization of “second generation” somatic hybrids derived from protoplast fusion between interspecific somatohaploid and dihaploidSolanum tuberosum L.

2000 ◽  
Vol 77 (3) ◽  
pp. 149-159 ◽  
Author(s):  
Veli-Matti Rokka ◽  
Jari P. T. Valkonen ◽  
Airi Tauriainen ◽  
Leena Pietilä ◽  
Renata Lebecka ◽  
...  
Keyword(s):  
Protoplast Fusion ◽  
Second Generation ◽  
Somatic Hybrids

scholarly journals Stomatal analysis of citrus somatic hybrids obtained by protoplast fusion

2004 ◽  
Vol 39 (3) ◽  
pp. 297-300 ◽  
Author(s):  
Maria Angélica Pereira de Carvalho Costa ◽  
Weliton Antonio Bastos de Almeida ◽  
Francisco de Assis Alves Mourão Filho ◽  
Beatriz Madalena Januzzi Mendes ◽  
Adriana Pinheiro Martinelli Rodriguez
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Protoplast Fusion ◽  
Somatic Hybrid ◽  
Somatic Hybrids ◽  
Morphological Characteristics ◽  
Leaf Epidermis ◽  
Young Leaves ◽  
Internal Arrangement ◽  
Scanning Electron

The objective of this work was to evaluate leaf epidermis morphological characteristics of three citrus somatic hybrids, compared to their parents. Parental and somatic hybrid young leaves were collected and processed for scanning electron microscope observations. Citrus polyploid hybrids have fewer stomata per area and these are larger compared to their diploid parental parents. No differences in internal arrangement of the stomatal cells were detected between parental plants and somatic hybrids. Additional studies may determine if these differences will influence physiological behavior of the plants in the field.

Development of an effective protoplast fusion system for production of new potatoes with disease and insect resistance using Mexican wild potato species as gene pools

2008 ◽  
Vol 88 (4) ◽  
pp. 611-619 ◽  
Author(s):  
Qin Chen ◽  
H. Y. Li ◽  
Y. Z. Shi ◽  
D. Beasley ◽  
B. Bizimungu ◽  
...  
Keyword(s):  
Late Blight ◽  
Protoplast Fusion ◽  
Insect Resistance ◽  
Colorado Potato Beetle ◽  
Wild Species ◽  
Somatic Hybrids ◽  
Fusion System ◽  
Cultivated Potato ◽  
Potato Species ◽  
Potato Beetle

Somatic hybridization through protoplast fusion is an important alternative approach for overcoming sexual incompatibility between diploid Solanum species and cultivated potatoes. However, compared with other potato species, methods for protoplast isolation and plant generation for several Mexican wild diploid potato species are not well established. In this study, a systematic procedure was designed for the isolation of a large number of high-quality protoplasts from various Mexican wild species that carry high levels of disease (late blight) and insect [Colorado potato beetle (CPB)] resistance. Using this procedure, an effective potato protoplast fusion system was developed to produce new somatic hybrids between two Mexican, one Argentina wild species, and cultivated potato clones. Regenerated plants and somatic hybrids were obtained at a high frequency from the protoplasts of the diploid wild species and their fused cells with S. tuberosum. Morphological, cytological and molecular marker analyses demonstrated that somatic hybrids were successfully obtained from the cell fusion of S. tuberosum and the diploid species S. pinnatisectum, S. cardiophyllum, and S. chacoense. Assessment of disease and insect reactions demonstrated that several of the protoplast-derived clones and somatic hybrids showed a higher level of resistance to both late blight and CPB than was found in S. tuberosum, confirming that the protoplast system is a powerful tool in potato breeding programs for the development of disease and insect resistance. This new fusion system provides breeders with opportunities to transfer disease and insect resistance genes from Mexican wild species into cultivated potato. Key words: Somatic hybrid, protoplast, fusion, potato, Solanum, late blight, disease resistance, Colorado potato beetle insect resistance

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