Preparation and properties of modified sol-gel sensing membrane for fiber optic oxygen sensor

2004 ◽  
Vol 19 (1) ◽  
pp. 19-22
Author(s):  
Huang Jun ◽  
Han Yun ◽  
Zhang Tian-hua ◽  
Jiang De-sheng ◽  
Yue Fang-yu
1994 ◽  
Author(s):  
Brian D. MacCraith ◽  
Gerard O'Keeffe ◽  
Aisling K. McEvoy ◽  
Colette M. McDonagh
Keyword(s):  

2002 ◽  
Vol 17 (2) ◽  
pp. 51-53 ◽  
Author(s):  
Jiang De-sheng ◽  
Chen Xing ◽  
Liu Er ◽  
Huang Jun

2010 ◽  
Author(s):  
Hai Wang ◽  
Jun Huang ◽  
Yinquan Yuan ◽  
Liyun Ding ◽  
Dian Fan

2012 ◽  
Vol 542-543 ◽  
pp. 620-624 ◽  
Author(s):  
Hua Fang ◽  
You Rong Wang

To detect DNA concentration, a novel fiber optic biosensor based on fluorescence quenching was designed and constructed by using lock-in amplifier for signal measurement. The sensor head was fixed on a sensing membrane prepared by the mixture of ethyl silicate, formamide, HCl and fluorescence indicator [Ru(phen)3]2+. The optimal film-forming conditions were that the contents of [Ru(phen)3]Cl2, HCl and formamide were 2.1 mg/ml, 12 % and 16 % respectively, the aging temperature and time of sol-gel solution were 4 oC and 5 d, and the film-forming temperature and time were 4 oC and 48 h, respectively. A linear relationship between the relative phase delay Δφ of sensing membrane and ctDNA concentration was observed in range from 2.0×10-9 to 4.0×10-7 M in Tris buffer, and the detection limit is 8.0×10-10 M. The biosensor had a response time of 65 s and showed good stability and repeatability.


2000 ◽  
Vol 42 (7-8) ◽  
pp. 283-290 ◽  
Author(s):  
H.-C. Tsai ◽  
R.-A. Doong

A sol-gel based fiber-optic biosensor with acetylcholinesterase as the biorecognition element has been developed for the rapid determination of organophosphorus pesticides. Nine fluorescent indicators, acridine, acridine orange, neutral red, DAPI, rhodamine B, fluorescein, umbelliferone, FITC on celite and FITC-dextran, have been examined to optimize the fiber-optic system. Results showed that acridine and FITCs were sensitive to the change of pH value caused by the enzyme-substrate catalysis reaction. However, the sensitivity of acridine was 260 times lower than that of FITCs. Higher toxicity of acridine to acetylcholinesterase than FITC was also observed. Moreover, the high-molecular-weight FITC-dextran showed low leakage rate when immobilizing using sol-gel technology, showing that the FITC-dextran was a suitable pH sensitive fluorescent indicator for the OPPs biosensor. The response of the fiber-optic biosensor to the substrate, acetylcholine, was highly reproducible (RSD=3.5%). A good linearity of acetylcholine in the range from 0.5 to 20 mM was also obtained (R2=0.98). Furthermore, a 30% inhibition can be achieved in 30min when 152 ppb paraoxon was added into the system. The results show the possibility for real-time determination of organophosphorus pesticides by using the biosensor developed in this study.


Sensors ◽  
2021 ◽  
Vol 21 (15) ◽  
pp. 5075
Author(s):  
Ondřej Podrazký ◽  
Jan Mrázek ◽  
Jana Proboštová ◽  
Soňa Vytykáčová ◽  
Ivan Kašík ◽  
...  

A practical demonstration of pH measurement in real biological samples with an in-house developed fiber-optic pH sensor system is presented. The sensor uses 8-hydroxypyrene-1,3,6-trisulfonate (HPTS) fluorescent dye as the opto-chemical transducer. The dye is immobilized in a hybrid sol-gel matrix at the tip of a tapered optical fiber. We used 405 nm and 450 nm laser diodes for the dye excitation and a photomultiplier tube as a detector. The sensor was used for the measurement of pH in human aqueous humor samples during cataract surgery. Two groups of patients were tested, one underwent conventional phacoemulsification removal of the lens while the other was subjected to femtosecond laser assisted cataract surgery (FLACS). The precision of the measurement was ±0.04 pH units. The average pH of the aqueous humor of patients subjected to FLACS and those subjected to phacoemulsification were 7.24 ± 0.17 and 7.31 ± 0.20 respectively.


2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Zizheng Yue ◽  
Wenlin Feng

Abstract In this work, a fiber-optic fluoride-ion-detection Michelson interferometer based on the thin-core fiber (TCF) and no-core fiber (NCF) coated with α-Fe2O3/ZrO2 sensing film is proposed and presented. The single-mode fiber (SMF) is spliced with the TCF and NCF in turn, and a waist-enlarged taper is spliced between them. Then, a silver film is plated on the end face of NCF to enhance the reflection. After the absorption of fluoride ion by the sensing film, the effective refractive index (RI) of the coated cladding will change, which leads to the regular red shift of the interference dip with the increasing fluoride-ion concentration. Thus, the fluoride-ion concentrations can be determined according to the corresponding dip wavelength shifts. The results show that the sensor has an excellent linear response (R 2 = 0.995) with good sensitivity (8.970 nm/ppm) when the fluoride-ion concentration is in the range of 0–1.5 ppm. The response time is about 15 s. The sensor has the advantage of good selectivity, good temperature and pH stabilities, and can be applied to detect fluoride ion effectively.


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