Clinical and enzymological studies on BSP metabolism (II) determination of relative storage capacity and secretory transport maximum of BSP, and the estimation of BSP conjugating enzyme activity of hepatic needle biopsy specimen

1966 ◽  
Vol 1 (1) ◽  
pp. 46-47
Author(s):  
I. Urushihata ◽  
K. Maeda ◽  
J. Nakagawa ◽  
K. Ito ◽  
T. Miyake
Keyword(s):  
Enzyme Activity ◽  
Needle Biopsy ◽  
Biopsy Specimen ◽  
Storage Capacity ◽  
Relative Storage Capacity ◽  
Secretory Transport ◽  
Conjugating Enzyme

Determination of bromsulphthalein-glutathione conjugating enzyme activity in needle biopsy specimens of human liver

1973 ◽  
Vol 47 (2) ◽  
pp. 133-137 ◽  
Author(s):  
D.V. Datta ◽  
Sarban Singh ◽  
A.K.S. Samanta ◽  
S. Saha ◽  
M. Mukherjee ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Needle Biopsy ◽  
Human Liver ◽  
Biopsy Specimens ◽  
Conjugating Enzyme

scholarly journals Aspek Histopatologik Adenokarsinoma Prostat

Medicinus ◽  
2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Erna Kristiani
Keyword(s):  
Prostate Carcinoma ◽  
Needle Biopsy ◽  
Biopsy Specimen ◽  
Tumor Staging ◽  
Prostate Adenocarcinoma ◽  
Biopsy Needle ◽  
Keywords Prostate ◽  
Morphologic Variation ◽  
Biopsy Score

<p><strong>Introduction: </strong>Prostate carcinoma is the second most common tumor in male, 95% in which made up from adenocarcinoma. The diagnosis of prostate adenocarcinoma through a needle biopsy specimen plus the determination of tumor staging are paramount in selecting the therapy and management. This study is done to know the morphologic variation of prostate adenocarcinoma in the needle biopsy as well as to measure the grading compatibility between the needle biopsy and prostatectomy using Gleason scoring system.</p><p><strong>Materials and Method: </strong>This retrospective study is conducted through form and specimen slides compilement. The specimens, consisting of prostate adenocarcinoma’s needle biopsy and prostatectomy, were gathered from the archives of Pathological Anatomy Departement, Faculty of Medicine, University of Indonesia in the year of 2008-2013. The slides were re-read and the morphologic appearance’s variation was valued. Gleason scoring was also executed from the pairing specimen according to <em>International Society of Urological Pathology</em> (ISUP) 2010.</p><p><strong>Result: </strong>Out of 114 needle biopsy cases, the morphologic variation was found to be perineural invation (n=38), mucinous fibroplasia (n=1), glomerulation (n=1), mucinous basophilic (n=25), and eosinophilic crystal (n=5). The amount of patient that was performed both specimen is 11, and there is a compatibility between biopsy score and prostatectomy as much as 63.63% and the median is 7.</p><p><strong>Conclusion: </strong>It is requisite to know the morphological variation in prostate adenocarcinoma in the biopsy needle specimen to get an accurate diagnosis. Undergrading in biopsy specimen is as much as 36.36%, owing to the fact that prostate carcinoma can be manifested as mutifocal lesion, thus the higher grading can only be found in prostatectomy specimen, for the needle biopsy was inadequately taken.</p><p><strong>Keywords: prostate adenocarcinoma, morphological view, Gleason score</strong></p>

Determination of sulfobromophthalein excretion in rabbits

1988 ◽  
Vol 254 (6) ◽  
pp. R1043-R1046
Author(s):  
J. M. Quale ◽  
N. V. Bergasa ◽  
L. J. Mandel
Keyword(s):  
Storage Capacity ◽  
Simple Technique ◽  
Biliary Tree ◽  
Hepatic Uptake ◽  
Direct Measurements ◽  
Relative Storage Capacity ◽  
Surgical Cannulation

The excretion of sulfobromophthalein (BSP) is determined by hepatic uptake (storage capacity) and secretion (transport maximum). We calculated the BSP excretion data in six rabbits by measuring the rate of rise of BSP at various infusion rates. The relative storage capacity was determined to be 0.34 +/- 0.13 mg.mg-1.dl-1.kg-1. The transport maximum was calculated to be 0.91 +/- 0.12 mg.min-1.kg-1. These results compare closely with previously reported results using more direct measurements that require anesthesia and surgical cannulation of the biliary tree. Repeated studies were easily performed. Our method provides a reliable and simple technique to study the effects of various conditions or substances on BSP excretion in conscious, unanesthetized rabbits.

A Rapid Electrophoretic Method for the Determination of the Isoenzymes of Serum Lactate Dehydrogenase

1966 ◽  
Vol 12 (5) ◽  
pp. 308-313 ◽  
Author(s):  
Albert W Opher ◽  
Charles S Collier ◽  
Joseph M Miller
Keyword(s):  
Enzyme Activity ◽  
Lactate Dehydrogenase ◽  
Quantitative Determination ◽  
Serum Lactate ◽  
Serum Lactate Dehydrogenase ◽  
Electrophoretic Method ◽  
The Individual ◽  
Electrophoretic Procedure ◽  
Purple Formazan

Abstract A convenient electrophoretic procedure for the separation and quantitation of lactate dehydrogenase (LDH) isoenzymes is described. The system uses polyacetate Sepraphore III strips.* The areas of activity are shown by incubation with an LDH substrate combined with tetra-nitro-blue-tetrazolium. The reduction of the latter to the purple formazan is quantitatively related to the enzyme activity. Quantitative determination of the individual colored areas is performed by densitometry.

EUTHYROID GOITERS IN CHILDREN: CORRELATION OF NEEDLE BIOPSY WITH OTHER CLINICAL AND LABORATORY FINDINGS IN CHRONIC LYMPHOCYTIC THYROIDITIS AND SIMPLE GOITER

PEDIATRICS ◽  
1969 ◽  
Vol 44 (5) ◽  
pp. 695-708
Author(s):  
Shun M. Ling ◽  
Solomon A. Kaplan ◽  
Jordan J. Weitzman ◽  
George B. Reed ◽  
Gertrude Costin ◽  
...  
Keyword(s):  
Thyroid Function ◽  
Needle Biopsy ◽  
Biopsy Specimen ◽  
Disease Process ◽  
Chronic Lymphocytic Thyroiditis ◽  
Red Cell ◽  
Thyroid Antibodies ◽  
Thyroid Extract ◽  
Laboratory Findings ◽  
Lymphocytic Thyroiditis

All children with euthyroid goiters examined over a period of 3 years underwent study of thyroid function, needle biopsy, and measurement of thyroid antibodies. Sixty-six of 71 children studied had satisfactory biopsies of the thyroid. Of these, 43 (65%) had chronic lymphocytic thyroiditis and 23 (35%) had simple goiter as determined by histologic examination of the biopsy specimen. Elevated titers of anti-thyroid antibodies were found in 50% of the patients with thyroiditis by the tanned red cell method and in 63% by the indirect Coons method. Neither antibody was present in significant quantity in 20% of the patients. Abnormal levels of antibody were rarely detected in children with histologic evidence of simple goiter. Treatment with thyroid extract has little effect on the progression of the disease process to fibrosis and atrophy. Such treatment may be necessary to anticipate the development of hypothyroidism or for replacement if thyroid function is already diminished. There is no evidence that simple goiter is associated with hypothyroidism, and treatment of this disease with thyroid preparations is necessary only for cosmetic purposes. Needle biopsy is the most useful means for making a distinction between thyroiditis and simple goiter.

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