A micro-scale method for determining relative metal-binding affinities of proteins

1997 ◽  
Vol 8 (3) ◽  
pp. 215-218 ◽  
Author(s):  
Jens Sommer-Knudsen ◽  
Antony Bacic
Keyword(s):  
Metal Binding ◽  
Binding Affinities ◽  
Micro Scale ◽  
Scale Method

Antimicrobial Activity of Formylchromones: Detection by a Micro-Scale Method

2011 ◽  
Vol 66 (11-12) ◽  
pp. 562-570
Author(s):  
Markku Lehtinen ◽  
Eila Pelttari ◽  
Hannu Elo
Keyword(s):  
Antimicrobial Activity ◽  
High Throughput Screening ◽  
Test Substance ◽  
Minimal Inhibitory Concentrations ◽  
Micro Scale ◽  
Drug Candidates ◽  
Scale Method ◽  
Deep Well ◽  
Quinolone Antibiotics ◽  
Microbial Suspension

We report the antimicrobial activity of formylchromones. These compounds are remote structural analogues of nalidixic acid and quinolone antibiotics, and their activity was investigated by a simple micro-scale method designed for the determination of minimal inhibitory concentrations (MIC) of drug candidates and antibiotics against aerobic bacteria and yeasts. Minimal bactericidal and fungicidal concentrations (MBC and MFC, respectively) were also determined in connection with the MIC determinations. The results obtained were compared with those obtained using classical agar diffusion methodology. In the MIC method, deep-well micro-titration plates are used, covered by silicone sealing mats that allow diffusion of oxygen to the wells. The appropriate broth is pipetted into the wells, followed by a standardized microbial suspension (except for sterile controls) and a dilution series of the test substance or control antibiotic or a mere control solvent. The use of white nontransparent polypropylene plates allows easy visual inspection of microbial growth. For the MBC and MFC methods, samples are taken from all wells that contain a test substance or control antibiotic and do not display growth in the MIC test. The samples are streaked on agar plates, the liquid is allowed to absorb into the agar, and finally the microbes are spread all over the plate with a bent rod. Colony counts are compared with that of the untreated microbial suspension at the beginning of the MIC test. The MIC method is suitable for high-throughput screening

The effect of non-coordinating side chains on the metal binding affinities of peptides of histidine

Polyhedron ◽  
2013 ◽  
Vol 62 ◽  
pp. 7-17 ◽  
Author(s):  
Ildikó Turi ◽  
Daniele Sanna ◽  
Eugenio Garribba ◽  
Giuseppe Pappalardo ◽  
Imre Sóvágó
Keyword(s):  
Metal Binding ◽  
Side Chains ◽  
Binding Affinities

Liquid-chromatographic assay of cimetidine in plasma and gastric fluid.

1985 ◽  
Vol 31 (4) ◽  
pp. 621-623 ◽  
Author(s):  
M Abdel-Rahim ◽  
D Ezra ◽  
C Peck ◽  
J Lazar
Keyword(s):  
Aqueous Phase ◽  
Internal Standard ◽  
Gastric Fluid ◽  
Radial Compression ◽  
Micro Scale ◽  
Scale Method ◽  
Large Numbers ◽  
Chromatographic Measurement ◽  
Liquid Chromatographic

Abstract In this micro-scale method for liquid-chromatographic measurement of cimetidine in 250-microL volumes of plasma or gastric fluid, a combination of organic- and aqueous-phase extractions, and protonation of the internal standard and cimetidine, enabled us to detect 2.0 ng of cimetidine on the column. We also used a radial compression module, which reduced the retention times for cimetidine and the internal standard to only 2.78 and 2.00 min. The speed and sensitivity of this method facilitates analysis of large numbers of samples.

Metal Binding Affinities ofArabidopsisZinc and Copper Transporters: Selectivities Match the Relative, but Not the Absolute, Affinities of their Amino-Terminal Domains,

Biochemistry ◽  
2009 ◽  
Vol 48 (49) ◽  
pp. 11640-11654 ◽  
Author(s):  
Matthias Zimmermann ◽  
Oliver Clarke ◽  
Jacqui M. Gulbis ◽  
David W. Keizer ◽  
Renee S. Jarvis ◽  
...  
Keyword(s):  
Metal Binding ◽  
Binding Affinities ◽  
Amino Terminal ◽  
Copper Transporters ◽  
The Absolute ◽  
Terminal Domains

Micro-scale method for theophylline in body fluids by reversed-phase, high-pressure liquid chromatography.

1977 ◽  
Vol 23 (3) ◽  
pp. 599-601 ◽  
Author(s):  
J J Orcutt ◽  
P P Kozak ◽  
S A Gillman ◽  
L H Cummins
Keyword(s):  
Sodium Acetate ◽  
Internal Standard ◽  
Peak Height ◽  
Reversed Phase ◽  
Acetate Buffer ◽  
Sodium Acetate Buffer ◽  
Serum Theophylline ◽  
Micro Scale ◽  
Scale Method ◽  
Standard Beta

Abstract We describe a micro-scale method for determining serum theophylline. The chromatography system includes a muBondapack C18 column and acetonitrile, 70 ml/liter of sodium acetate buffer (10 mmol/liter, ph 4.0) as the mobile phase. Test serum or plasma, 30 mul, is mixed with an equal quantity of a solution containing the internal standard, beta-hydroxyethyltheophylline in acetonitrile/sodium acetate buffer (20 mmol/liter, pH 4.0), 7/43 by vol. After the precipitate is removed by centrifugation, the mixture is chromatographed and the amount of theophylline calculated from the ratio between peak heights for theophylline and the internal standard. Advantages include easy sample preparation, involving only addition of internal standard and centrifugation before injection, long column life, and the suitability of the internal standard, which is adjusted to a peak height equivalent to 20 mg of theophylline per liter for easy computation of results.

A micro-scale method for liquid scintillation counting, applicable to radioimmunoassay of steroids and substances of comparable relative molecular mass.

1978 ◽  
Vol 24 (7) ◽  
pp. 1193-1195 ◽  
Author(s):  
H E Grotjan ◽  
E Steinberger
Keyword(s):  
Molecular Mass ◽  
Liquid Scintillation Counting ◽  
Liquid Scintillation ◽  
Scintillation Counting ◽  
Usual Method ◽  
Relative Molecular Mass ◽  
Micro Scale ◽  
Scale Method ◽  
Double Antibody ◽  
The Cost

Abstract A major expense of radioimmunoassay involving tritiated tracers is that of liquid scintillation counting. We present a micro-scale method that markedly reduces the cost of liquid scintillation counting. The radioimmunoassay is done in 10 X 50 mm tubes. The antibody-bound tracer is precipitated with a second antibody, the precipitate is resuspended in 0.1 ml of water, and 0.3 ml of dioxane is added. One milliliter of a toluene-based scintillation cocktail is added, and the tube is capped and placed in an adapter for liquid scintilation counting. When this method was applied to a double-antibody testosterone radioimmunoassay, it performed comparably to assays counted by the usual method.

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