Statistical optimization of conditions for protease production fromBacillus sp. and its scale-up in a bioreactor

2007 ◽  
Vol 141 (2-3) ◽  
pp. 229-239 ◽  
Author(s):  
Saurabh Saran ◽  
Jasmine Isar ◽  
Rajendra Kumar Saxena
Keyword(s):  
Scale Up ◽  
Statistical Optimization ◽  
Protease Production

scholarly journals Keratinolytic Protease Production by Bacillus Cereus Strain Ps03 under Submerged Fermentation: Optimization and Characterization

2016 ◽  
Vol 4 (3) ◽  
pp. 397-401
Author(s):  
M.D. BalaKumaran ◽  
R. Santhi
Keyword(s):  
Bacillus Subtilis ◽  
Scale Up ◽  
Nitrogen Sources ◽  
Maximum Activity ◽  
Protease Production ◽  
Subtilis Strain ◽  
Physical Factors ◽  
Chicken Feather ◽  
Protease Enzyme ◽  
Keratinolytic Protease

In the present study, chicken feather powder was screened for its application as the substrate for the production of keratinolytic protease by Bacillus subtilis strain PS03. Bacillus subtilis produced a high level of keratinolytic protease using chicken feather powder as substrate. With feather powder as substrate, physical factors such as incubation time, pH and temperature were optimized for increased keratinolytic protease production by Bacillus subtilis. The enzyme production was enhanced when using maltose as carbon source and yeast extract as nitrogen sources. SDS-PAGE analysis indicated the molecular weight of 46 kDa of the partially purified keratinolytic protease. The keratinolytic protease enzyme was stable over a pH range of 6 – 9 and temperature range of 35 - 50°C with maximum activity at pH 9 and 40°C. Based on the results, the use of feather powder as substrate for keratinolytic protease production is cost effective and is easy to scale up. Considering the availability and cost, chicken feather powder is considered as an ideal substrate for keratinolytic protease production in an industrial point of view. Int J Appl Sci Biotechnol, Vol 4(3): 397-401

scholarly journals Sea Almond as a Promising Feedstock for Green Diesel: Statistical Optimization and Power Rate Law Based Chemical Kinetics of Its Consecutive Irreversible Methanolysis

Prunus ◽  
2020 ◽  
Author(s):  
Chizoo Esonye ◽  
Okechukwu Donminic Onukwuli ◽  
Akuzuo Uwaoma Ofoefule ◽  
Cyril Sunday Ume ◽  
Nkiruka Jacintha Ogbodo
Keyword(s):  
Kinetic Model ◽  
Chemical Kinetics ◽  
Seed Oil ◽  
Scale Up ◽  
Statistical Optimization ◽  
Industrial Scale ◽  
Order Kinetic ◽  
Rate Law ◽  
Power Rate ◽  
Order Kinetic Model

For successful industrial scale-up and effective cost analysis of transesterification process, presentation of complimentary research data from process optimization using statistical design techniques, chemical kinetics and thermodynamics are essential. Full factorial central composite design (FFCCD) was applied for the statistical optimization of base methanolysis of sea almond (Terminalia catappa) seed oil using response surface methodology (RSM) coupled with desirability function analysis on quadratic model. Reaction time had the most significant impact on the biodiesel yield. Optimum conditions for biodiesel yield of 93.09 wt% validated at 92.58 wt% were 50.03°C, 2.04 wt% catalyst concentration, 58.5 min and 4.66 methanol/oil molar ratio with overall desirability of 1.00. Ascertained fuel properties of the FAME were in compliance with international limits. GC–MS, FTIR and NMR characterizations confirmed unsaturation and good cold-flow qualities of the biodiesel. Based on power rate law, second-order kinetic model out-performed first-order kinetic model. Rate constants of the triglyceride (TG), diglycerides (DG) and monoglycerides (MG) hydrolysis were in the range of 0.00838–0.0409 wt%/min while activation energies were 12.76, 15.83 and 22.43 kcal/mol respectively. TG hydrolysis to DG was the rate determining step. The optimal conditions have minimal error and would serve as a springboard for industrial scale-up of biodiesel production from T. catappa seed oil.

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