Peanut agglutinin induced alterations in capsular and extracellular polysaccharide synthesis andex -planta nitrogenase activity of cowpea rhizobia

1987 ◽  
Vol 12 (3) ◽  
pp. 289-296 ◽  
Author(s):  
Bina Mody ◽  
Vinod Modi
2015 ◽  
Vol 18 (3) ◽  
pp. 904-922 ◽  
Author(s):  
Xingqun Cheng ◽  
Xin Zheng ◽  
Xuedong Zhou ◽  
Jumei Zeng ◽  
Zhi Ren ◽  
...  

2015 ◽  
Vol 21 ◽  
pp. 12-17
Author(s):  
I. M. Malinovska

It was established that Bacillus mucilaginosus C-3 requires introduction of nitrogen sourceinto the culture medium in form of nitrate as themost optimal for maximal accumulation of bacterial biomass and sporulation process. Introduction of the ammonium form was less efficient. It was shown that the intensity of nitrogensource use by B. mucilaginosus is highly depended on the physiological state of the inoculum cells. At sowing of spore inoculum theoptimal concentration of potassium nitrate was2.5 g/l resulting in 1010 cells/ml, while under theuse of vegetative cells the optimal concentrationof potassium nitrate was 1.0 g/l leading to theaccumulation of 109 cells/ml.Use of spore inoculum had ensured themaximum productivity of extracellular polysaccharide synthesis. With the higher number ofB. mucilaginosus subcultures in a vegetativestate the efficiency of the exopolysaccharidesynthesis has decreased: after the second passage — by 20.0 %, after the fourth — by 56.5 %,after the sixth — at 127.8 %. After eighth passage the culture loses its ability to synthesizepolysaccharide, especially on the medium withthe N : C ratio 1 : 3 and resume this ability onlyafter sporulation and the significant increase ofthe N : C ratio.


RSC Advances ◽  
2017 ◽  
Vol 7 (71) ◽  
pp. 45075-45084 ◽  
Author(s):  
Pei-pei Han ◽  
Shun-yu Yao ◽  
Rong-jun Guo ◽  
Rong-rong Yan ◽  
Yi-kai Wu ◽  
...  

Important enzymes influencing the production ofNostoc flagelliformeEPS were investigated under different culture conditions.


1992 ◽  
Vol 38 (9) ◽  
pp. 986-988 ◽  
Author(s):  
H. R. Barbosa ◽  
F. Alterthum

Cells of Beijerinckia derxii grown on NH4+ or N2 as the nitrogen source were treated to remove their extracellular polysaccharide layer without loss of viability. An adequate method for extracellular polysaccharide extraction was established. After extracellular polysaccharide removal N2-grown cells showed reduced nitrogenase activity, and when stirred for 90 min at 200 rpm, suffered a total loss of viability. In contrast, NH4+-grown cells were not affected when submitted to the same conditions. However, these cells showed a high sensitivity to saline solution. Key words: extracellular polysaccharide, nitrogenase, Beijerinckia derxii, exopolysaccharide.


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