Stimulation of extracellular polysaccharide synthesis in oat protoplasts by the host-specific phytotoxin victorin

Planta ◽  
1985 ◽  
Vol 165 (3) ◽  
pp. 407-415 ◽  
Author(s):  
Jonathan D. Walton ◽  
Elizabeth D. Earle
Keyword(s):  
Extracellular Polysaccharide ◽  
Polysaccharide Synthesis ◽  
Stimulation Of

Regulation of oxidative response and extracellular polysaccharide synthesis by a diadenylate cyclase inStreptococcus mutans

2015 ◽  
Vol 18 (3) ◽  
pp. 904-922 ◽  
Author(s):  
Xingqun Cheng ◽  
Xin Zheng ◽  
Xuedong Zhou ◽  
Jumei Zeng ◽  
Zhi Ren ◽  
...  
Keyword(s):  
Extracellular Polysaccharide ◽  
Polysaccharide Synthesis ◽  
Oxidative Response

scholarly journals EXOPOLYSACCHARIDE SYNTHESIS PRODUCTIVITY BY BACILLUS MUCILAGINOSUS DEPENDING ON THE NITROGEN SOURCE AND ORIGIN OF INOCULUM

2015 ◽  
Vol 21 ◽  
pp. 12-17
Author(s):  
I. M. Malinovska
Keyword(s):  
Nitrogen Source ◽  
Culture Medium ◽  
Physiological State ◽  
Bacterial Biomass ◽  
Extracellular Polysaccharide ◽  
Potassium Nitrate ◽  
Bacillus Mucilaginosus ◽  
Vegetative Cells ◽  
Polysaccharide Synthesis ◽  
Sporulation Process

It was established that Bacillus mucilaginosus C-3 requires introduction of nitrogen sourceinto the culture medium in form of nitrate as themost optimal for maximal accumulation of bacterial biomass and sporulation process. Introduction of the ammonium form was less efficient. It was shown that the intensity of nitrogensource use by B. mucilaginosus is highly depended on the physiological state of the inoculum cells. At sowing of spore inoculum theoptimal concentration of potassium nitrate was2.5 g/l resulting in 1010 cells/ml, while under theuse of vegetative cells the optimal concentrationof potassium nitrate was 1.0 g/l leading to theaccumulation of 109 cells/ml.Use of spore inoculum had ensured themaximum productivity of extracellular polysaccharide synthesis. With the higher number ofB. mucilaginosus subcultures in a vegetativestate the efficiency of the exopolysaccharidesynthesis has decreased: after the second passage — by 20.0 %, after the fourth — by 56.5 %,after the sixth — at 127.8 %. After eighth passage the culture loses its ability to synthesizepolysaccharide, especially on the medium withthe N : C ratio 1 : 3 and resume this ability onlyafter sporulation and the significant increase ofthe N : C ratio.

scholarly journals Influence of culture conditions on extracellular polysaccharide production and the activities of enzymes involved in the polysaccharide synthesis of Nostoc flagelliforme

RSC Advances ◽  
2017 ◽  
Vol 7 (71) ◽  
pp. 45075-45084 ◽  
Author(s):  
Pei-pei Han ◽  
Shun-yu Yao ◽  
Rong-jun Guo ◽  
Rong-rong Yan ◽  
Yi-kai Wu ◽  
...  
Keyword(s):  
Extracellular Polysaccharide ◽  
Culture Conditions ◽  
Nostoc Flagelliforme ◽  
Polysaccharide Production ◽  
Polysaccharide Synthesis

Important enzymes influencing the production ofNostoc flagelliformeEPS were investigated under different culture conditions.

Pullulan elaboration and differentiation of the resting forms in Aureobasidium pullulans

1995 ◽  
Vol 41 (1) ◽  
pp. 35-45 ◽  
Author(s):  
L. Simon ◽  
B. Bouchet ◽  
C. Caye-Vaugien ◽  
D. J. Gallant
Keyword(s):  
Cell Wall ◽  
Aureobasidium Pullulans ◽  
Culture Medium ◽  
Extracellular Polysaccharide ◽  
Growth Conditions ◽  
Cellular Level ◽  
Wall Thickening ◽  
Resting Forms ◽  
Polysaccharide Synthesis ◽  
Swollen Cell

To identify the cellular forms that are responsible for the synthesis of pullulan produced by Aureobasidium pullulans, we performed cytochemical and ultrastructural localizations of glucan in the cellular forms of this microorganism (blastospores and resting forms). Growth conditions, cell populations, and pullulan production were studied concurrently. Our results are consistent with a model in which the resting forms (swollen cells and chlamydospores) might be primarily involved in this extracellular polysaccharide elaboration. At the cellular level, pullulan production could be the result of three main stages: (i) cell wall thickening and extracellular polysaccharide synthesis by the swollen cell, (ii) fibrillar arrangement of this polysaccharide into pullulan along a capsular network around the chlamydospore, and (iii) subcellular hydrolysis separating the capsule from the periplasmic zone and consequently permitting the solubilization of pullulan in the culture medium. A melanization process in the outer layer of the cell wall and the capsule accompanies these patterns.Key words: Aureobasidium pullulans, capsule, cytochemistry, polysaccharide, pullulan, resting forms.

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