Genetic diversity amongst landraces of a dioecious vegetatively propagated plant, betelvine (Piper betle L.)

Paan (Piper betle L., family Piperaceae) is an important evergreen cultivated crop of India. We estimated genetic diversity by using morphological and RAPD markers in four cultivars, namely Piper betle L. var. Bali, var. Chandrakanta, var. Jhanji and var. Kala Bangla. We had used morphological markers like leaf length, leaf width, and petiole size. By using the morphological markers, hierarchical cluster analysis was carried out, which grouped these four cultivars into two major clusters. In molecular marker analysis, a total of ten RAPD primers used, generating 43 number of amplified bands. Among them, 15 number of polymorphic bands and seven unique bands were found. The genetic diversity and relatedness among the four cultivars were computed using Jaccard’s similarity coefficient. The dendrogram grouped all the four cultivars into two main clusters. This RAPD banding patterns can be useful for genetic diversity studies, for cultivar selection, and to marker assist breeding programs.

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ISSR marker based diversity assessment of Piper spp. in Bay Islands, India

Indian Journal of Agricultural Research ◽

10.18805/ijare.a-4941 ◽

2018 ◽

Author(s):

K. Abirami ◽

V. Baskaran ◽

P. Simhachalam

Keyword(s):

Genetic Diversity ◽

Germplasm Collection ◽

Inter Simple Sequence Repeat ◽

Rapid Identification ◽

The Other ◽

Issr Marker ◽

Piper Nigrum ◽

Piper Betle ◽

Diversity Assessment ◽

Bay Islands

Inter-simple sequence repeat (ISSR) analysis was used to study the genetic diversity of Piper spp. with its relative genus Peperomia pellucida. Among the 38 ISSR primers used, 10 primers generated 1083 bands, of which seven (80.8%) were polymorphic. The maximum polymorphism were obtained from the primers UBC 881, UBC 889, UBC 848 and UBC 830. Cluster analysis grouped the species studied into two major clusters. One cluster comprised the different accessions of Piper nigrum and is distinct from the other species of Piperaceae. The other cluster grouped the four species of Piperaceae namely Piper longum, Piper sarmentosum, Piper betle and Peperomia pellucida. The variability studies also revealed that the ISSR marker could group the accessions within the same species based on their similar geographical origin. Assessment of genetic diversity among the different species of the family Piperaceae through ISSR marker would help in rapid identification of polymorphism, assist in future germplasm collection, conservation and domestication programmes.

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Genetic diversity analysis of Piper betle from eight accessions of Indonesia based on SRAP markers

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d220837 ◽

2021 ◽

Vol 22 (8) ◽

Author(s):

Sharah Nabilla ◽

Ukhradiya Magharaniq Safira ◽

Puspa Julistia Puspita ◽

Dyah Subositi ◽

Anshary Maruzy ◽

...

Keyword(s):

Genetic Diversity ◽

Diversity Index ◽

Folk Medicine ◽

Dna Amplification ◽

Scientific Data ◽

Piper Betle ◽

Diversity Analysis ◽

Srap Marker ◽

Genetic Diversity Analysis ◽

Srap Markers

Abstract. Nabilla S, Safira UM, Puspita PJ, Subositi D, Maruzy A, Artika IM. 2021. Genetic diversity analysis of Piper betle from eight accessions of Indonesia based on SRAP markers. Biodiversitas 22: 3401-3408. Leaves of betel vine (Piper betle Linn.) have been used as a traditional medicine in various regions in Indonesia. However, the genetic diversity of this plant has not been well recorded. Considering its diverse usage in traditional and folk medicine, it is essential to analyze and document the genetic diversity of Piper betle L. with the aim to collect the scientific data of betel vine genetics in Indonesia. This study aims at analyzing the population structure and genetic diversity of betel vine from the Singkil, Gayo Serbajadi, Baduy, Bandung, Hutan, Kalisusu, Kaledupa, and Mekongga accession groups of Indonesia using the sequence-related-amplified polymorphism (SRAP) marker technique. Genomic DNA was isolated from each accession and then used as a template for DNA amplification using PCR. As many as 16 SRAP primer combinations were screened for the genetic diversity analysis. Optimization of primer combinations resulted in 7 selected combinations based on their ability to generate clear amplification patterns and polymorphic bands. These were then employed for genetic diversity analysis. The genetic distance dendrogram showed the lowest similarity coefficient was 0.62 and that the betel vine grouping pattern was not based on genotype. The Singkil population had the highest genetic diversity and the Hutan population had the lowest. The mean value of Nei's genetic diversity index was 0.0985, while Shannon's information index was 0.01459 and the percentage of polymorphic loci was 25.81. This study concluded that the level of betel vine diversity is low.

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