Comparison of various analytical techniques for the determination of essential fatty acids in hardened fats

1966 ◽  
Vol 43 (7) ◽  
pp. 425-428 ◽  
Author(s):  
Helen Zmachinski ◽  
Arthur Waltking ◽  
James D. Miller
Keyword(s):  
Fatty Acids ◽  
Essential Fatty Acids ◽  
Analytical Techniques

Determination of Essential Fatty Acids in Popular Olive Cultivars Grown in Saudi Arabia

2013 ◽  
Vol 12 (3) ◽  
pp. 155-162 ◽  
Author(s):  
Ibrahim M. Alruqaie ◽  
F.A. Al Ghamidi ◽  
Hend A. AbuHaimed
Keyword(s):  
Fatty Acids ◽  
Saudi Arabia ◽  
Essential Fatty Acids ◽  
Olive Cultivars

scholarly journals Differential Incorporation of Alpha-Linolenic Acid Into Phospholipid Classes in H4IIE Cells

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 491-491
Author(s):  
Danielle Defries ◽  
Kayla Curtis ◽  
Shiva Shariati ◽  
Heather Blewett ◽  
Michel Aliani
Keyword(s):  
Fatty Acids ◽  
Linolenic Acid ◽  
Essential Fatty Acids ◽  
Analytical Techniques ◽  
Fatty Acyl ◽  
Alpha Linolenic Acid ◽  
Funding Sources ◽  
H4iie Cells ◽  
Fatty Acyls

Abstract Objectives Alpha-linolenic acid (ALA) is an essential fatty acid found in plant-based oils, with health benefits attributed to its metabolic conversion to very-long chain polyunsaturated fatty acids. Other routes of cellular ALA metabolism exist, but much of our knowledge of ALA metabolism stems from dated analytical techniques. Essential fatty acids are present in cells primarily as fatty acyls, glycerolipids, and glycerophospholipids, but finer details of ALA metabolism remain unexplored. The current study used unbiased metabolomics to profile the ALA metabolites produced in liver cells after treatment with ALA. Methods H4IIE hepatoma cells were incubated with or without 60 μM ALA for 0, 15, 30, 60, 120, 180, 260, 480, and 720 minutes.  Samples were extracted and prepared for HPLC/LC-QTOF-MS analysis.  MS data acquisitions were completed in both positive and negative modes.  Data were analyzed with 2-way ANOVA (ALA and time as factors) followed by Bonferroni FWER corrections for multiple comparisons. Differences with p < 0.05 were considered significant. Results Among the metabolites detected, differences in patterns of ALA-containing phospholipids (PLs) were observed with ALA treatment. Consistent with a high proportion of phosphatidylcholine (PC) in mammalian cell membranes, ALA-containing PCs such as PC(18:3/18:3), PC(18:3/18:4), lysoPC(18:3/0:0), and PC(18:3/18:1) were significantly higher in ALA-treated cells at most time points.  In addition, several phosphatidylethanolamine (PE) species with ALA were significantly higher in ALA-treated cells, including PE(18:3/18:3), PE(18:3/18:2), PE(18:3/16:0), PE(18:3/20:5), and PE(18:3/22:6).  To a lesser degree, ALA was also found in phosphatidylserine (PS)(18:3/20:5), lysoPS(18:3/0:0), lysophosphatidylglycerol (18:3/0:0) and phosphatidic acid (PA)(18:3/18:2) and PA(18:3/22:6), suggesting that ALA incorporation into PLs is not limited to PC, contrary to the current known pathways of ALA metabolism. Conclusions Based on these results, there appears to be a preference for incorporation of ALA into PC and PE; however, ALA is also incorporated into PS, PG, and PA. Given the role of different PLs, and functionality based on their fatty acyl composition, future studies will explore the functional outcomes of ALA incorporation into different PL classes. Funding Sources NSERC Discovery Grant

Determination of Essential Fatty Acids in Captured and Farmed Matrinxã (Brycon cephalus) from the Brazilian Amazonian Area

2009 ◽  
Vol 86 (8) ◽  
pp. 717-722 ◽  
Author(s):  
Neiva Maria Almeida ◽  
Natália Soares Janzantti ◽  
Maria Regina Bueno Franco
Keyword(s):  
Fatty Acids ◽  
Essential Fatty Acids

scholarly journals Fatty Acid Profile of Roots and Aerial Parts of Ruscus hyrcanus Woronow

2019 ◽  
Vol 25 (1) ◽  
pp. 78-81 ◽  
Author(s):  
Mir Babak Bahadori ◽  
Solmaz Asnaashari ◽  
Hossein Nazemiyeh
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Essential Fatty Acids ◽  
Fatty Acid Analysis ◽  
Aerial Parts ◽  
Esterified Fatty Acids ◽  
First Time

Background: Ruscus specie are used as traditional medicine, food, and foliage. The aim of this work is the determination of fatty acid composition of Ruscus hyrcanus as a native medicinal plant of Iran for the first time together with comparison of different esterification methods. Methods: Two different esterification methods were used for preparation of esterified fatty acids from different extracts of underground and aerial parts of the herb. GC/MS analysis were used for identification and quantification of fatty acids. Finally, the results were compared. Results: Findings showed that R. hyrcanus is rich in essential fatty acids such as linoleic acid (13-25%) and linolenic acid (23-44%). Also, oil samples contain remarkable amount of palmitic acid (19-57%). Conclusion: The results showed that R. hyrcanus could be considered as a source of essential fatty acids. Also, it could be concluded that a simple esterification method with methanolic KOH and 2 min vortex is suitable for fatty acid analysis of Ruscus species.

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