Laser-induced photodynamic effects of chlorinse 6 andp 6 on an isolated neuron

1999 ◽  
Vol 66 (2) ◽  
pp. 268-273
Author(s):  
A. B. Uzdenskii ◽  
A. F. Mironov ◽  
A. P. Losev
Keyword(s):  
Isolated Neuron

Isolated Neuron Preparation for Studies of Metabolic Events at Rest and during Impulse Activity

Science ◽  
1963 ◽  
Vol 140 (3562) ◽  
pp. 74-75 ◽  
Author(s):  
E. Giacobini ◽  
E. Handelman ◽  
C. A. Terzuolo
Keyword(s):  
Impulse Activity ◽  
Isolated Neuron

scholarly journals Endocannabinoid- and mGluR5-Dependent Short-Term Synaptic Depression in an Isolated Neuron/Bouton Preparation From the Hippocampal CA1 Region

2008 ◽  
Vol 100 (2) ◽  
pp. 1041-1052 ◽  
Author(s):  
Anton Sheinin ◽  
Giuseppe Talani ◽  
Margaret I. Davis ◽  
David M. Lovinger
Keyword(s):  
Hippocampal Neurons ◽  
Basolateral Amygdala ◽  
Pyramidal Neurons ◽  
Metabotropic Glutamate Receptor ◽  
Hippocampal Slices ◽  
Neuronal Membrane ◽  
Short Term ◽  
Ca1 Region ◽  
Hippocampal Ca1 Region ◽  
Isolated Neuron

Endocannabinoids released from the postsynaptic neuronal membrane can activate presynaptic CB1 receptors and inhibit neurotransmitter release. In hippocampal slices, depolarization of the CA1 pyramidal neurons elicits an endocannabinoid-mediated inhibition of γ-aminobutyric acid release known as depolarization-induced suppression of inhibition (DSI). Using the highly reduced neuron/synaptic bouton preparation from the CA1 region of hippocampus, we have begun to examine endocannabinoid-dependent short-term depression (STD) of inhibitory synaptic transmission under well-controlled physiological and pharmacological conditions in an environment free of other cells. Application of the CB1 synthetic agonist WIN55212 -2 and endogenous cannabinoids 2-AG and anandamide produced a decrease in spontaneous inhibitory postsynaptic current (sIPSC) frequency and amplitude, indicating the presence of CB1 receptors at synapses in this preparation. Endocannabinoid-dependent STD is different from DSI found in hippocampal slices and the neuron/bouton preparation from basolateral amygdala (BLA) since depolarization alone was not sufficient to induce suppression of sIPSCs. However, concurrent application of the metabotropic glutamate receptor (mGluR) agonist ( RS)-3,5-dihydroxyphenylglycine (DHPG) and postsynaptic depolarization resulted in a transient (30–50 s) decrease in sIPSC frequency and amplitude. Application of DHPG alone had no effect on sIPSCs. The depolarization/DHPG-induced STD was blocked by the CB1 antagonist SR141716A and the mGluR5 antagonist MPEP and was sensitive to intracellular calcium concentration. Comparing the present findings with earlier work in hippocampal slices and BLA, it appears that endocannabinoid release is less robust in isolated hippocampal neurons.

A planar microelectrode array for simultaneous detection of electrically evoked dopamine release from distinct locations of a single isolated neuron

The Analyst ◽  
2013 ◽  
Vol 138 (10) ◽  
pp. 2833 ◽  
Author(s):  
Bhavik Anil Patel ◽  
Collin C. Luk ◽  
Pei Ling Leow ◽  
Arthur J. Lee ◽  
Wali Zaidi ◽  
...  
Keyword(s):  
Dopamine Release ◽  
Microelectrode Array ◽  
Simultaneous Detection ◽  
Isolated Neuron

PACEMAKER PLASTICITY IN ISOLATED NEURON

1981 ◽  
pp. 7-12
Author(s):  
E.N. Sokolov ◽  
T.N. Grechenko
Keyword(s):  
Isolated Neuron

Peculiarities of studying an isolated neuron by the method of laser interference microscopy

2006 ◽  
Vol 36 (9) ◽  
pp. 874-878 ◽  
Author(s):  
Alexander I Yusipovich ◽  
Sergey M Novikov ◽  
Tatiana A Kazakova ◽  
Liudmila A Erokhova ◽  
Nadezda A Brazhe ◽  
...  
Keyword(s):  
Interference Microscopy ◽  
Laser Interference ◽  
Isolated Neuron ◽  
Laser Interference Microscopy

ATPase and carbonic anhydrase activities of bulk-isolated neuron, glia and synaptosome fractions from rat brain

1978 ◽  
Vol 141 (2) ◽  
pp. 305-323 ◽  
Author(s):  
H.K. Kimelberg ◽  
S. Biddlecome ◽  
S. Narumi ◽  
R.S. Bourke
Keyword(s):  
Carbonic Anhydrase ◽  
Rat Brain ◽  
Isolated Neuron

scholarly journals The self-referencing oxygen-selective microelectrode: detection of transmembrane oxygen flux from single cells

1999 ◽  
Vol 202 (2) ◽  
pp. 211-218
Author(s):  
S.C. Land ◽  
D.M. Porterfield ◽  
R.H. Sanger ◽  
P.J. Smith
Keyword(s):  
Real Time ◽  
Single Cells ◽  
Light Response ◽  
The Self ◽  
Oxygen Flux ◽  
Metabolic Substrates ◽  
Translational Movement ◽  
Isolated Neuron ◽  
Time Changes ◽  
High Level

A self-referencing, polarographic, oxygen-selective microelectrode was developed for measuring oxygen fluxes from single cells. This technique is based on the translational movement of the microelectrode at a known frequency through an oxygen gradient, between known points. The differential current of the electrode was converted into a directional measurement of flux using the Fick equation. Operational characteristics of the technique were determined using artificial gradients. Calculated oxygen flux values matched theoretical values derived from static measurements. A test preparation, an isolated neuron, yielded an oxygen flux of 11.46+/−1.43 pmol cm-2 s-1 (mean +/− s.e.m.), a value in agreement with those available in the literature for single cells. Microinjection of metabolic substrates or a metabolic uncoupler increased oxygen flux, whereas microinjection of KCN decreased oxygen flux. In the filamentous alga Spirogyra greveilina, the probe could easily differentiate a 16.6% difference in oxygen flux with respect to the position of the spiral chloroplast (13.3+/−0.4 pmol cm-2 s-1 at the chloroplast and 11.4+/−0.4 pmol cm-2 s-1 between chloroplasts), despite the fact that these positions averaged only 10.6+/−1.8 microm apart (means +/− s.e.m.). A light response experiment showed real-time changes in measured oxygen flux correlated with changes in lighting. Taken together, these results show that the self-referencing oxygen microelectrode technique can be used to detect local oxygen fluxes with a high level of sensitivity and spatial resolution in real time. The oxygen fluxes detected reliably correlated with the metabolic state of the cell.

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