A planar microelectrode array for simultaneous detection of electrically evoked dopamine release from distinct locations of a single isolated neuron

The Analyst ◽  
2013 ◽  
Vol 138 (10) ◽  
pp. 2833 ◽  
Author(s):  
Bhavik Anil Patel ◽  
Collin C. Luk ◽  
Pei Ling Leow ◽  
Arthur J. Lee ◽  
Wali Zaidi ◽  
...  
Keyword(s):  
Dopamine Release ◽  
Microelectrode Array ◽  
Simultaneous Detection ◽  
Isolated Neuron

scholarly journals Microfabricated FSCV-compatible microelectrode array for real-time monitoring of heterogeneous dopamine release

The Analyst ◽  
2010 ◽  
Vol 135 (7) ◽  
pp. 1556 ◽  
Author(s):  
Matthew K. Zachek ◽  
Jinwoo Park ◽  
Pavel Takmakov ◽  
R. Mark Wightman ◽  
Gregory S. McCarty
Keyword(s):  
Real Time ◽  
Dopamine Release ◽  
Microelectrode Array ◽  
Real Time Monitoring

Microelectrode Array Biochip:  Tool for In Vitro Drug Screening Based on the Detection of a Drug Effect on Dopamine Release from PC12 Cells

2006 ◽  
Vol 78 (18) ◽  
pp. 6347-6355 ◽  
Author(s):  
Hui-Fang Cui ◽  
Jian-Shan Ye ◽  
Yu Chen ◽  
Ser-Choong Chong ◽  
Fwu-Shan Sheu
Keyword(s):  
Pc12 Cells ◽  
Drug Screening ◽  
Dopamine Release ◽  
Microelectrode Array ◽  
Drug Effect ◽  
In Vitro Drug Screening

Species Specific Immunoassays to Measure Blood Platelet and Coagulation Activation in the Rat

1996 ◽  
Vol 76 (06) ◽  
pp. 1090-1095 ◽  
Author(s):  
C Ravanat ◽  
M Freund ◽  
S Schuhler ◽  
P Grunert ◽  
L Meyer ◽  
...  
Keyword(s):  
Antithrombin Iii ◽  
Plasma Concentrations ◽  
Simultaneous Detection ◽  
Blood Platelet ◽  
Coagulation Activation ◽  
Platelet Factor ◽  
Prethrombotic State ◽  
Low Levels ◽  
Species Specific ◽  
Higher Sensitivity

SummaryThe purpose of this study was to develop specific and sensitive immunoassays to detect early indices of hypercoagulability in the rat. Rat platelet factor 4 (rPF4) and rat fibrinopeptide A (rFPA) assays, tools for the detection of activation of platelets and coagulation respectively, were designed using antibodies raised against purified rPF4 and against synthetic rFPA. The relevance of these new assays and of the commercially available ELISA kit for thrombin-antithrombin III (TAT) complexes was demonstrated in a rat model of a prethrombotic state induced by intravenous infusion of varying doses of thromboplastin (90 to 2400 μl/kg/h). In this model, the immunoassays allowed simultaneous detection of low levels of rFPA and rPF4 which were correlated with fibrinogen and platelet consumption and TAT generation and further proved to be of higher sensitivity than the classical methods of platelet count or measurement of fibrinogen levels. Plasma concentrations of rFPA, rPF4 and TAT were dependent on infusion time and thromboplastin dose, while hirudin (1 mg/kg) prevented their appearance. Thus the new specific immunoassays for rPF4 and rFPA and the commercial human TAT assay represent useful tools for pathophysiological studies or the screening of antithrombotic drugs in rats.

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