Chromatographic isolation of the original constituents of natural waxes. The composition of ouricuri wax

1960 ◽  
Vol 37 (7) ◽  
pp. 359-364 ◽  
Author(s):  
Leslie J. N. Cole ◽  
J. B. Brown
Keyword(s):  
Chromatographic Isolation

Chromatographic Isolation of the LDH-Isoenzymes of Human Blood Platelets and an Investigation of Their Enzyme Kinetics

1968 ◽  
Vol 20 (03/04) ◽  
pp. 301-313 ◽  
Author(s):  
W Schneider ◽  
K Schumacher ◽  
B Thiede ◽  
R Gross
Keyword(s):  
Human Blood ◽  
Blood Platelets ◽  
Deae Cellulose ◽  
Order Reaction ◽  
Kinetic Properties ◽  
Ldh Isoenzymes ◽  
Kinetic Investigations ◽  
Substrate Concentrations ◽  
Chromatographic Isolation ◽  
Human Blood Platelets

SummaryThe LDH-isoenzymes of human blood platelets show a distinct predominance of the isoenzymes 2 and 3 upon chromatography on DEAE-cellulose. Small amounts of LDH-1 are also present, while only traces of LDH-4 and -5 can be detected.Enzyme kinetic investigations of the principal isoenzymes LDH-1, -2 and -3 clearly show that the differences in inhibition constants with pyruvate as substrate which are demonstrable at 25° largely disappear at 37°. On the other hand, the differences among the isoenzymes in their affinity for pyruvate and lactate as substrate as well as in with respect to the optimal substrate concentrations of pyruvate are more marked at 37° than at 25°. Also, the type of inhibition found with lactate as substrate is increasingly the expression of a higher order reaction in going from LDH-1 to LDH-3. A dependence of the LDH distribution pattern upon the metabolism of the cell is discussed. A comparison of our results with thrombocytes with those of other workers with erythrocytes and leucocytes makes it unlikely that the LDH pattern is directly dependent upon the existence of an oxidative metabolism. Rather, the redox potential of the cell could be of importance for the nature of the pattern of isoenzymes and for their differing kinetic properties.

Ion-exchange high-performance liquid chromatographic isolation of the major allergen of parietaria pollen extract

1990 ◽  
Vol 512 ◽  
pp. 115-121 ◽  
Author(s):  
Ib Søndergaard ◽  
Paolo Falagiani ◽  
Ezio Bolzacchini ◽  
Paola Civaroli ◽  
Guiseppina di Gregorio ◽  
...  
Keyword(s):  
Ion Exchange ◽  
High Performance ◽  
Major Allergen ◽  
High Performance Liquid Chromatographic ◽  
Pollen Extract ◽  
Liquid Chromatographic ◽  
Chromatographic Isolation

Chromatographic Isolation of Presumptive Peptide Flavor Principles from Red Meat

1987 ◽  
Vol 10 (12) ◽  
pp. 2745-2758 ◽  
Author(s):  
Arthur M. Spanier ◽  
Judson V. Edwards
Keyword(s):  
Red Meat ◽  
Chromatographic Isolation
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