Chromatographic isolation and concentration of triterpene alcohols on columns with Polikhrom-1 and Polisorb-4T

1984 ◽  
Vol 20 (5) ◽  
pp. 637-637
Author(s):  
T. V. Pokushalova ◽  
L. I. Glebko
1968 ◽  
Vol 20 (03/04) ◽  
pp. 301-313 ◽  
Author(s):  
W Schneider ◽  
K Schumacher ◽  
B Thiede ◽  
R Gross

SummaryThe LDH-isoenzymes of human blood platelets show a distinct predominance of the isoenzymes 2 and 3 upon chromatography on DEAE-cellulose. Small amounts of LDH-1 are also present, while only traces of LDH-4 and -5 can be detected.Enzyme kinetic investigations of the principal isoenzymes LDH-1, -2 and -3 clearly show that the differences in inhibition constants with pyruvate as substrate which are demonstrable at 25° largely disappear at 37°. On the other hand, the differences among the isoenzymes in their affinity for pyruvate and lactate as substrate as well as in with respect to the optimal substrate concentrations of pyruvate are more marked at 37° than at 25°. Also, the type of inhibition found with lactate as substrate is increasingly the expression of a higher order reaction in going from LDH-1 to LDH-3. A dependence of the LDH distribution pattern upon the metabolism of the cell is discussed. A comparison of our results with thrombocytes with those of other workers with erythrocytes and leucocytes makes it unlikely that the LDH pattern is directly dependent upon the existence of an oxidative metabolism. Rather, the redox potential of the cell could be of importance for the nature of the pattern of isoenzymes and for their differing kinetic properties.


2017 ◽  
pp. 15-21
Author(s):  
Thi Minh Nguyet Le ◽  
Thi Kim Lien Phan

Background and Objectives: For a long time, Asian women have known to use rice water, rice bran for skin care, lightening dark spots and slowing down the aging process. Gamma Oryzanol, an ester mixture of trans-ferulic acid with phytosterols and triterpene alcohols extracted from rice bran oil, has been shown to have beneficial effects on the skin, especially its antioxidant and anti-aging effect. The aims of this research were to formulate Gamma Oryzanol-loaded anti-aging cream and to evaluate the product quality. Materials and Methods: Gamma Oryzanol was imported from Japan. Some formulation parameters such as emulsifi-ers, performance modifiers were investigated and the product quality was evaluated according to the criteria of drugs and cosmetics. Results: The excipients were chosen including 1% (w/w) of Lunamer with 3% (w/w) of Prolipid as emulsifiers and 8% (w/w) of mango seed butter as a state modifier in the formulation of Gamma Oryzanol anti-aging cream. The cream is ivory-white, thick, smooth, fragrant-smelling and satisfied all criteria of developed specification. Conclusion: The study was successful in the preparation of Gamma Oryzanol anti-aging cream. Key words: Gamma Oryzanol, anti-aging cream, Franz cells.


1978 ◽  
Vol 80 (10) ◽  
pp. 382-386 ◽  
Author(s):  
T. Itoh ◽  
T. Tamura ◽  
T. Matsumoto

1990 ◽  
Vol 512 ◽  
pp. 115-121 ◽  
Author(s):  
Ib Søndergaard ◽  
Paolo Falagiani ◽  
Ezio Bolzacchini ◽  
Paola Civaroli ◽  
Guiseppina di Gregorio ◽  
...  

1986 ◽  
Vol 22 (4) ◽  
pp. 487-487 ◽  
Author(s):  
V. I. Roshchin ◽  
O. Yu. Poverinova ◽  
V. A. Raldugin ◽  
V. A. Pentegova

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