Aflatoxin in Arizona cottonseed: Field inoculation of bolls byAspergillus flavusspores in wind-driven soil

1986 ◽  
Vol 63 (4) ◽  
pp. 530-532 ◽  
Author(s):  
L. S. Lee ◽  
L. V. Lee ◽  
T. E. Russell
Keyword(s):  
Field Inoculation

ASSESSMENT OF NITROGEN FIXATION POTENTIAL IN AHIPA (Pachyrhizus ahipa) AND ITS EFFECT ON ROOT AND SEED YIELD

2009 ◽  
Vol 45 (2) ◽  
pp. 177-188 ◽  
Author(s):  
D. N. RODRÍGUEZ-NAVARRO ◽  
M. CAMACHO ◽  
F. TEMPRANO ◽  
C. SANTAMARÍA ◽  
E. O. LEIDI
Keyword(s):  
Raw Materials ◽  
Farming Systems ◽  
Yield Potential ◽  
Small Scale ◽  
South American ◽  
Field Inoculation ◽  
Promising Alternative ◽  
Yield Increase ◽  
Alternative Crop ◽  
Attractive Option

SUMMARYAhipa is a legume of great interest for the production of raw materials (starch, sugar, oil and proteins) for industrial use. Its yield potential and ability to fix atmospheric N2 in association with rhizobia makes it an attractive option for low input agriculture systems. At present, it is cultivated on a very small scale as a food crop in a few South American countries. Little information is available on symbiotic N2 fixation in ahipa and no work has been performed on strain selection for inoculant production. Soils in southwest Europe are devoid of specific rhizobia able to nodulate on ahipa. Selecting rhizobia for symbiotic effectiveness from a collection led to the isolation of strains which provided greater shoot growth and N content under controlled conditions. In the field, inoculation at sowing with the selected strains increased significantly seed and tuberous root yield and seed protein content. The amount of N2 fixed, estimated by 15N natural abundance, reached 160–260 kg N ha−1. In previous work, ahipa appeared to be a promising alternative crop for the production of industrial raw materials. The results of the present study showed a yield increase in tuberous roots and seeds when applying effective rhizobia inoculants. Furthermore, a positive soil N balance was left after its cultivation making ahipa even more interesting for sustainable farming systems.

scholarly journals Effects of Field Inoculation with VAM and Bacteria Consortia on Root Growth and Nutrients Uptake in Common Wheat

2018 ◽  
Vol 10 (9) ◽  
pp. 3286 ◽  
Author(s):  
Cristian Dal Cortivo ◽  
Giuseppe Barion ◽  
Manuel Ferrari ◽  
Giovanna Visioli ◽  
Lucia Dramis ◽  
...  
Keyword(s):  
Common Wheat ◽  
Root Length Density ◽  
N Uptake ◽  
Mycorrhizal Fungus ◽  
Growth Yield ◽  
N Fertilization ◽  
Environmental Benefits ◽  
Root Tip ◽  
Field Inoculation ◽  
N Dose

This study investigated the effects of a commercial biofertilizer containing the mycorrhizal fungus Rhizophagus irregularis and the diazotrophic N-fixing bacterium Azotobacter vinelandii on root and shoot growth, yield, and nutrient uptake in common wheat (Triticum aestivum L.) in order to improve the sustainable cultivation of this widespread crop. The trials were carried out in controlled conditions (rhizoboxes) and in open fields over two years to investigate the interaction between inoculation and three doses of nitrogen fertilization (160, 120 and 80 kg ha−1) in a silty-loam soil of the Po Plain (NE Italy). In rhizoboxes, efficient root colonization by R. irregularis was observed at 50 days after sowing with seed inoculation, together with improved root tip density and branching (+~30% vs. controls), while the effects of post-emergence inoculation by soil and foliar spraying were not observable at plant sampling. In the open, field spraying at end tillering significantly increased the volumetric root length density (RLD, +22% vs. controls) and root area density (+18%) after about two months (flowering stage) in both years under medium and high N fertilization doses, but not at the lowest N dose. In absence of inoculation, RLD progressively decreased with increased N doses. Inoculation had a negligible effect on grain yield and N uptake, which followed a typical N dose-response model, while straw Zn, P, and K concentrations were seldom improved. It is concluded that medium-high N fertilization doses are required to achieve the target yield and standards of quality (protein contents) in wheat cultivation, while the use of this mixed VAM-PGPR biofertilizer appears to be a sustainable mean for minimizing the adverse effects of chemical N fertilizers on root expansion and for improving the uptake of low-mobility nutrients, which has potentially relevant environmental benefits.

Open field inoculation with PGPR as a strategy to manage fertilization of ancient Triticum genotypes

2019 ◽  
Vol 56 (1) ◽  
pp. 111-124 ◽  
Author(s):  
Giancarlo Pagnani ◽  
Angelica Galieni ◽  
Fabio Stagnari ◽  
Marika Pellegrini ◽  
Maddalena Del Gallo ◽  
...  
Keyword(s):  
Open Field ◽  
Field Inoculation

scholarly journals Seed Coating with Arbuscular Mycorrhizal Fungi for Improved Field Production of Chickpea

Agronomy ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 471 ◽  
Author(s):  
Inês Rocha ◽  
Isabel Duarte ◽  
Ying Ma ◽  
Pablo Souza-Alonso ◽  
Aleš Látr ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal ◽  
Field Conditions ◽  
Growth And Yield ◽  
Seed Coating ◽  
Agricultural Practice ◽  
Agricultural Crops ◽  
Field Inoculation ◽  
Inoculation Methods ◽  
Fungal Isolates ◽  
The Impact

Although arbuscular mycorrhizal (AM) fungi are known to promote growth and yield of agricultural crops, inoculation methods for effective scaling up from greenhouse to the field are still underexplored. The application of single or mixed beneficial AM fungal isolates is hindered by the lack of experimental reproducibility of findings at different scales and the cost-effectivity of inoculation methods. Seed coating has been considered a feasible delivery system of AM fungal inocula for agricultural crops. In this study, the impact of single and multiple AM fungal isolates applied via seed coating on chickpea productivity was evaluated under greenhouse and field conditions. Overall, plants inoculated with multiple AM fungal isolates had better performance than those inoculated with single AM isolate under greenhouse and field conditions. While plants in greenhouse displayed higher shoot dry weight (14%) and seed individual weight (21%), in field, inoculation with multiple AM isolates increased pod (160%), and seed (148%) numbers, and grain yield (140%). Under field conditions, mycorrhizal root colonization was significantly higher in chickpea plants inoculated with multiple AM fungal isolates compared to other treatments. These findings highlight the potential of field-inoculation with multiple AM fungal isolates via seed coating as a sustainable agricultural practice for chickpea production.

Field inoculation with arbuscular mycorrhizal fungi in rehabilitation of mine sites with native vegetation, including Acacia spp.

2003 ◽  
Vol 16 (1) ◽  
pp. 131 ◽  
Author(s):  
J. Bell ◽  
S. Wells ◽  
D. A. Jasper ◽  
L. K. Abbott
Keyword(s):  
Mycorrhizal Fungi ◽  
Field Experiments ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Native Plant ◽  
Field Inoculation ◽  
Native Plant Species ◽  
Mycorrhizal Colonisation ◽  
Mine Sites ◽  
Fertiliser Application

Field experiments were conducted at rehabilitation sites at two contrasting mines in Western Australia. At both mines, Acacia spp. are important components of the rehabilitation ecosystem. At a mineral sands mine near Eneabba, dry-root inoculum of the arbuscular mycorrhizal (AM) fungus Glomus invermaium (WUM 10) was introduced into riplines with three rates of phosphate fertiliser application. Plants were assessed for mycorrhizal colonisation and phosphorus status. There was no plant growth benefit from inoculation. A considerable number of infective propagules of indigenous AM fungi was already present in the topsoil. The inoculant fungus as well as the indigenous AM fungi formed mycorrhizas, but only in a small number of Acacia and other native plant species. In a study of AM fungal inoculation at a gold mine rehabilitation site at Boddington, dry-root inoculum of G.�invermaium was applied to riplines prior to seeding. Despite apparently ideal environmental conditions, colonisation of native seedlings was limited. Possible reasons for this were investigated in further experiments that addressed environmental factors such as soil temperature and moisture and factors such as the age of the plant and presence of a colonised cover crop. Inoculum remained infective even under moist conditions in field soil for at least 4 months. Its infectivity decreased in parallel with falling temperatures. However, the level of infectivity present did not ensure extensive colonisation of native plants such as Acacia seedlings in the field. Susceptibility of Acacia seedlings to colonisation by AM fungi appeared to be seasonal, as colonisation increased with increasing daytime temperatures and daylight hours.

Nitrogen fixation (acetylene reduction) by lines composing 'Park' Kentucky bluegrass

1986 ◽  
Vol 32 (4) ◽  
pp. 348-352
Author(s):  
Keuk-Ki Lee ◽  
Robert C. Shearman ◽  
Robert V. Klucas
Keyword(s):  
Root Exudates ◽  
Nitrogenase Activity ◽  
Soluble Sugar ◽  
Kentucky Bluegrass ◽  
Selected Lines ◽  
Field Inoculation ◽  
Nitrogenase Activities ◽  
Highly Correlated ◽  
Hydroponically Grown

Response of 'Park' Kentucky bluegrass to inoculation with Klebsiella pneumoniae strain W-6 was tested under field conditions. Field inoculation did not increase nitrogenase activity measured in situ, but did increase the nitrogenase activity as measured using an excised root assay which included a 10-h incubation before the addition of acetylene. Fifteen lines composing 'Park' were grown in fritted-clay medium, inoculated with soil, and compared for nitrogenase activities using the excised roots assay. Significant differences were observed between two lines. Six lines were selected from the 15 lines, grown hydroponically, inoculated with soil, and assayed for nitrogenase activity using intact 105-day-old plants. Nitrogenase activities were immediately detectable and increased curvilinearly. Differences in nitrogenase activities among the six lines were detected within 1 h and significant differences were evident in 4 h. The six selected lines were also tested for levels of anthrone-reactive sugars in roots and root exudates of hydroponically grown plants. Significant differences were detected in levels of sugars in roots but not in root exudates. Possible correlations were sought among and within the three different experiments involving the six selected lines. With hydroponically grown plants, nitrogenase activities during the first 4 h were highly correlated with those after 24 h incubation. Correlations were found between nitrogenase activities in excised roots and soluble sugar concentrations in root tissue and root exudates.

A comparison of field inoculation methods with Cercosporella herpotrichoides (Fron) on spring barley

1969 ◽  
Vol 73 (3) ◽  
pp. 437-444 ◽  
Author(s):  
D. Gareth Jones ◽  
J. M. L. Davies
Keyword(s):  
Grain Yield ◽  
Disease Severity ◽  
Disease Incidence ◽  
Spring Barley ◽  
Barley Variety ◽  
Field Inoculation ◽  
Inoculation Methods ◽  
Field Plots

SUMMARYA comparison was made of six different methods of inoculation with Cercosporella herpotrichoidesFron on field plots of the spring barley variety Impala. All inoculation treatments produced significant differences in terms of disease incidence and grain yield compared with the uninoculated controls. Significant differences were found between treatments in terms of disease incidence, disease severity, mean yield/head and 1000 grain weights. The time of inoculation was also found to be significant. The earlier inoculation caused a reduction in yield of approximately 66 % compared with 50 % at the later inoculation. The application of these methods is discussed.

scholarly journals FIELD INOCULATION TESTS TO QUANTIFY RESISTANCE IN SPINACH TO RACES 3 AND 4 OF DOWNY MILDEW

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 598b-598
Author(s):  
L.P. Brandenberger ◽  
J.C. Correll ◽  
T.E. Morelock ◽  
R.W. McNew
Keyword(s):  
Downy Mildew ◽  
Disease Severity ◽  
Leaf Surface ◽  
Field Inoculation ◽  
Disease Progress Curve ◽  
Disease Progress ◽  
Progress Curve

Resistance to race 3 and 4 of downy mildew (Peronospora farinosa f.sp. spinaciae) was examined in separate field inoculation tests. Three Arkansas cultivars and three other commercial spinach cultivars were compared by periodically scoring individual leaves for disease severity 7 to 28 days after inoculation. Leaves were scored on a 0 to 6 scale with 0 = 0% of the leaf surface being covered with sporulation and 6 = 90-100%. Resistance was evaluated by comparing disease ratings on a given day as well as the area under the disease progress curve. Arkansas spinach cultivars exhibited significantly lower disease severity ratings in field inoculation tests for all sample dates for both races 3 and 4 when compared to known susceptible cultivars.

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