Field inoculation with arbuscular mycorrhizal fungi in rehabilitation of mine sites with native vegetation, including Acacia spp.

2003 ◽  
Vol 16 (1) ◽  
pp. 131 ◽  
Author(s):  
J. Bell ◽  
S. Wells ◽  
D. A. Jasper ◽  
L. K. Abbott
Keyword(s):  
Mycorrhizal Fungi ◽  
Field Experiments ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Native Plant ◽  
Field Inoculation ◽  
Native Plant Species ◽  
Mycorrhizal Colonisation ◽  
Mine Sites ◽  
Fertiliser Application

Field experiments were conducted at rehabilitation sites at two contrasting mines in Western Australia. At both mines, Acacia spp. are important components of the rehabilitation ecosystem. At a mineral sands mine near Eneabba, dry-root inoculum of the arbuscular mycorrhizal (AM) fungus Glomus invermaium (WUM 10) was introduced into riplines with three rates of phosphate fertiliser application. Plants were assessed for mycorrhizal colonisation and phosphorus status. There was no plant growth benefit from inoculation. A considerable number of infective propagules of indigenous AM fungi was already present in the topsoil. The inoculant fungus as well as the indigenous AM fungi formed mycorrhizas, but only in a small number of Acacia and other native plant species. In a study of AM fungal inoculation at a gold mine rehabilitation site at Boddington, dry-root inoculum of G.�invermaium was applied to riplines prior to seeding. Despite apparently ideal environmental conditions, colonisation of native seedlings was limited. Possible reasons for this were investigated in further experiments that addressed environmental factors such as soil temperature and moisture and factors such as the age of the plant and presence of a colonised cover crop. Inoculum remained infective even under moist conditions in field soil for at least 4 months. Its infectivity decreased in parallel with falling temperatures. However, the level of infectivity present did not ensure extensive colonisation of native plants such as Acacia seedlings in the field. Susceptibility of Acacia seedlings to colonisation by AM fungi appeared to be seasonal, as colonisation increased with increasing daytime temperatures and daylight hours.

scholarly journals Field Inoculation of Arbuscular Mycorrhizal Fungi Improves Fruit Quality and Root Physiological Activity of Citrus

Agriculture ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1297
Author(s):  
Ming-Ao Cao ◽  
Peng Wang ◽  
Abeer Hashem ◽  
Stephan Wirth ◽  
Elsayed Fathi Abd_Allah ◽  
...  
Keyword(s):  
Protein Content ◽  
Fruit Quality ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Poncirus Trifoliata ◽  
Soluble Solids ◽  
Field Inoculation ◽  
Root Vitality ◽  
Soil Protein

Soil arbuscular mycorrhizal (AM) fungi form a mutualistic symbiosis with plant roots and produce many benefits on host plants under potted conditions, while field inoculation of AM fungi on citrus (a woody plant) has been rarely reported. The present study aimed to analyze the changes in mycorrhizal growth, root vitality, and fruit quality of Citrus reticulata Blanco var. Ponkan mandarin cv. Jinshuigan grafted on Poncirus trifoliata L. after inoculation with a mix of AM fungi (Diversispora versiformis, Funneliformis mosseae, and Rhizophagus intraradices) and single F. mosseae. After the second year of AM fungal inoculations, root mycorrhizal colonization (%), root vitality, hyphal length in soil, and easily extractable glomalin-related soil protein content were significantly increased, while difficult-to-extract glomalin-related soil protein content was decreased. Two mycorrhizal fungal inoculation treatments collectively improved fruit quality parameters such as polar diameter, equatorial diameter, the weight of single fruits, fruit peel, and sarcocarp, coloration value, and soluble solids content. Our study, therefore, suggested that field inoculation with AM fungi improved root physiological activities in terms of mycorrhizal growth and root vitality and thus improved fruit quality. The effect of mixed-AM treatment was more significant than that of F. mosseae alone.

scholarly journals Disentangling nematode and arbuscular mycorrhizal fungal community effect on the growth of range-expanding Centaurea stoebe in original and new range soil

2021 ◽  
Author(s):  
Kadri Koorem ◽  
Rutger A. Wilschut ◽  
Carolin Weser ◽  
Wim H. van der Putten
Keyword(s):  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Plant Performance ◽  
Native Plant ◽  
Soil Organisms ◽  
Centaurea Stoebe ◽  
Native Plant Species ◽  
Soil Communities ◽  
Plant Soil ◽  
Amf Communities

Abstract Aims Numerous organisms show range expansions in response to current climate change. Differences in expansion rates, such as between plants and soil biota, may lead to altered interactions in the new compared to the original range. While plant-soil interactions influence plant performance and stress tolerance, the roles of specific soil organisms driving these responses remain unknown. Methods We manipulated the abundances of nematodes and arbuscular mycorrhizal fungi (AMF), collected from original and new range soils, and examined their effects on the biomass of range-expanding Centaurea stoebe and native Centaurea jacea. In the first approach, nematode and AMF communities were extracted from field soils, and inoculated to sterilized soil. In the second approach, the abundance of soil organisms in soil inocula was reduced by wet sieving; at first, plants were grown to condition the soil, and then plant-soil feedback was determined under ambient and drought conditions. Results The origin of soil communities did not influence the biomass production of range-expanding or native plant species, neither by addition nor by (partial) removal. However, after conditioning and under drought, range expanding C. stoebe produced more biomass with soil communities from the original range while C. jacea, native to both ranges, produced more biomass with new range soil communities. Conclusions We show that nematode and AMF communities from original and new range have similar effect on the growth of range expanding C. stoebe. Our results highlight that the effect of soil communities on plant growth increases after soil conditioning and under drought stress.

scholarly journals EVALUATION OF THE INFLUENCE OF ARBUSCULAR MYCORRHIZAL FUNGI ON ECONOMICALLY VALUABLE INDICATORS OF ONION

Ekosistemy ◽  
2020 ◽  
Vol - (21) ◽  
pp. 101-108
Author(s):  
S. F. Abdurashytov ◽  
V. I. Nemtinov ◽  
E. V. Puzanova ◽  
K. S. Gritsevich ◽  
I. V. Belova ◽  
...  
Keyword(s):  
Mycorrhizal Fungi ◽  
Field Experiments ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Field Studies ◽  
Dry Mass ◽  
Initial Assessment ◽  
Vegetable Crops ◽  
Bulb Formation ◽  
Southern Chernozem

Allium cepa L. is one of the main vegetable crops. It is actively used in food industry and medicine. The biochemical composition of plants, both bulbs and green leaves, in different periods of growth changes depending on the variety, environmental and agrotechnical conditions of plant cultivation. Representatives of the genus Allium are highly sensitive to the presence of arbuscular mycorrhiza (AM) in soil due to root development features. The aim of our work was to identify effective associations of AM fungi to increase the productivity and quality of onions in the conditions of southern chernozem. Pot and field experiments were carried out with associations of AM fungi and onion varieties from the collection of the Research Institute of Agriculture of Crimea. The initial assessment of the AM fungi collection was conducted in the pot with a sterile substrate under artificial lighting conditions according to the intensity of mycorrhizal colonization and the accumulation of phytomass of the storage plant. Field studies were carried out on southern carbonate chernozem in 2019. It is established that the AM fungi association M9 significantly exceeds the referent in the arbuscules abundance by 13.3 %. Association 1–16 showed the most considerable abundance of vesicles, which was 11.1–13.3 % higher than other variants of this experiment. The dry mass of onion shoots had a significant increase of 11.719.7 mg per plant (38.865.8 %) from inoculation by associations AM S1-4, 1-16 and M9. The positive effect of the AM fungi association 1-16 on the accumulation of phytomass in the onset phase of bulb formation and onion productivity of the onion variety Yaltinskiy plus and line 11A with an increase by 0.690.8 g / plant (31.351.7 %) and 1.11.3 t / ha (14.114.9 %), respectively, and an increase in the content of ascorbic acid by 18.524.4 % to the variant without treatment are shown.

scholarly journals Interactions of arbuscular mycorrhizal fungi, critical loads of nitrogen deposition, and shifts from native to invasive species in a southern California shrubland

Botany ◽  
2016 ◽  
Vol 94 (6) ◽  
pp. 425-433 ◽  
Author(s):  
Edith B. Allen ◽  
Louise M. Egerton-Warburton ◽  
Bridget E. Hilbig ◽  
Justin M. Valliere
Keyword(s):  
Invasive Species ◽  
Mycorrhizal Fungi ◽  
Vegetation Type ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
N Deposition ◽  
Coastal Sage Scrub ◽  
Native Plant ◽  
Invasive Grass ◽  
Type Conversion

Anthropogenic nitrogen (N) deposition and invasive species are causing declines in global biodiversity, and both factors impact the diversity and functioning of arbuscular mycorrhizal (AM) fungi. Shifts in arbuscular mycorrhizal fungal (AMF) communities can generate feedback to native plants and affect their success, as was observed in California’s coastal sage scrub, which is a Mediterranean-type shrubland threatened by invasive grasses. As vegetation-type conversion from native shrubland to exotic annual grassland increased along a gradient of increasing N deposition, the richness of native plant species and of spore morphotypes decreased. Rapid declines in all plant and fungal values occurred at the critical load (CL) of 10–11 kg N·ha−1·year−1, indicating that AM fungi respond to the same environmental signals as the plants, and can be used to assess CL. Shrub root colonization also decreased along the N gradient, but colonization of the invasive grass was dominated by a fine AMF endophyte that was unresponsive to elevated N. A greenhouse experiment to assess AMF functioning showed that the native shrub Artemisia californica Less. had a negative growth response to an inoculum from high-N but not low-N soils, whereas the invasive grass Bromus rubens L. had a positive response to both inocula. Differential functioning of AM fungi under N deposition may in part explain vegetation-type conversion and the decline of this native shrubland.

scholarly journals Use of the Signature Fatty Acid 16:1ω5 as a Tool to Determine the Distribution of Arbuscular Mycorrhizal Fungi in Soil

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Christopher Ngosong ◽  
Elke Gabriel ◽  
Liliane Ruess
Keyword(s):  
Fatty Acid ◽  
Mycorrhizal Fungi ◽  
Plant Root ◽  
Background Level ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Biomass Estimation ◽  
Lipid Fatty Acid ◽  
Neutral Lipid Fatty Acid ◽  
Root Symbionts

Biomass estimation of arbuscular mycorrhiza (AM) fungi, widespread plant root symbionts, commonly employs lipid biomarkers, predominantly the fatty acid 16:1ω5. We briefly reviewed the application of this signature fatty acid, followed by a case study comparing biochemical markers with microscopic techniques in an arable soil following a change to AM non-host plants after 27 years of continuous host crops, that is, two successive cropping seasons with wheat followed by amaranth. After switching to the non-host amaranth, spore biomass estimated by the neutral lipid fatty acid (NLFA) 16:1ω5 decreased to almost nil, whereas microscopic spore counts decreased by about 50% only. In contrast, AM hyphal biomass assessed by the phospholipid (PLFA) 16:1ω5 was greater under amaranth than wheat. The application of PLFA 16:1ω5 as biomarker was hampered by background level derived from bacteria, and further enhanced by its incorporation from degrading spores used as microbial resource. Meanwhile, biochemical and morphological assessments showed negative correlation for spores and none for hyphal biomass. In conclusion, the NLFA 16:1ω5 appears to be a feasible indicator for AM fungi of the Glomales group in the complex field soils, whereas the use of PLFA 16:1ω5 for hyphae is unsuitable and should be restricted to controlled laboratory studies.

scholarly journals Response of Arbuscular Mycorrhizal Fungi to Simulated Climate Changes by Reciprocal Translocation in Tibetan Plateau

2015 ◽  
Vol 43 (2) ◽  
pp. 488-493
Author(s):  
Zhaoyong SHI ◽  
Xubin YIN ◽  
Bede MICKAN ◽  
Fayuan WANG ◽  
Ying ZHANG ◽  
...  
Keyword(s):  
Tibetan Plateau ◽  
Arbuscular Mycorrhizal Fungi ◽  
Reciprocal Translocation ◽  
Mycorrhizal Fungi ◽  
Climate Changes ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Spore Density ◽  
The Tibetan Plateau ◽  
Colonization Frequency

Arbuscular mycorrhiza (AM) fungi are considered as an important factor in predicting plants and ecosystem responses to climate changes on a global scale. The Tibetan Plateau is the highest region on Earth with abundant natural resources and one of the most sensitive region to climate changes. To evaluate the complex response of arbuscular mycorrhizal fungi colonization and spore density to climate changes, a reciprocal translocation experiment was employed in Tibetan Plateau. The reciprocal translocation of quadrats to AM colonization and spore density were dynamic. Mycorrhizal colonization frequency presented contrary changed trend with elevations of quadrat translocation. Colonization frequency reduced or increased in majority quadrats translocated from low to high or from high to low elevation. Responses of colonization intensity to translocation of quadrats were more sensitive than colonization frequency. Arbuscular colonization showed inconsistent trend in increased or decreased quadrat. Vesicle colonization decreased with changed of quadrat from low to high elevations. However, no significant trend was observed. Although spore density was dynamic with signs of decreasing or increasing in translocated quadrats, the majority enhanced and declined respectively in descent and ascent quadrat treatments. It is crucial to understand the interactions between AM fungi and prairie grasses to accurately predict effects of climate change on these diverse and sensitive ecosystems. This study provided an opportunity for understanding the effect of climate changes on AM fungi.

scholarly journals Synergistic community responses of plants and arbuscular mycorrhizal fungi to extreme droughts in a cold-temperate grassland

2020 ◽  
Author(s):  
Wei Fu ◽  
Baodong Chen ◽  
Matthias Rillig ◽  
Wang Ma ◽  
Chong Xu ◽  
...  
Keyword(s):  
Fungal Community ◽  
Mycorrhizal Fungi ◽  
Community Dynamics ◽  
Environmental Changes ◽  
Environmental Filtering ◽  
Climate Extremes ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Temperate Grassland ◽  
Community Responses

Mutualistic associations between plants and arbuscular mycorrhizal (AM) fungi may have profound influences on their response to climate changes. Existing theories evaluate the effects of interdependency and environmental filtering on plant-AM fungal community dynamics separately; however, abrupt environmental changes such as climate extremes can provoke duo-impacts on the metacommunity simultaneously. Here, we experimentally tested the relevance of plant and AM fungal community responses to extreme drought (chronic or intense) in a cold temperate grassland. Irrespective of drought intensities, plant species richness and productivity responses were significantly and positively correlated with AM fungal richness and also served as best predictors of AM fungal community shifts. Notably, the robustness of this community synergism increased with drought intensity, likely reflecting increased community interdependence. Network analysis showed a key role of Glomerales in AM fungal interaction with plants, suggesting specific plant-AM fungal pairing. Thus, community interdependence may underpin climate change impact on plant-AM fungal diversity patterns in grasslands.

scholarly journals The Synergy of Arbuscular Mycorrhizal Fungi and Exogenous Abscisic Acid Benefits Robinia pseudoacacia L. Growth through Altering the Distribution of Zn and Endogenous Abscisic Acid

2021 ◽  
Vol 7 (8) ◽  
pp. 671
Author(s):  
Xiao Lou ◽  
Xiangyu Zhang ◽  
Yu Zhang ◽  
Ming Tang
Keyword(s):  
Abscisic Acid ◽  
Mycorrhizal Fungi ◽  
Black Locust ◽  
Robinia Pseudoacacia ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Am Symbiosis ◽  
Antioxidant Defense Systems ◽  
Robinia Pseudoacacia L ◽  
Zn Stress

The simultaneous effects of arbuscular mycorrhizal (AM) fungi and abscisic acid (ABA) on the tolerance of plants to heavy metal (HM) remain unclear. A pot experiment was carried out to clarify the effects of simultaneous applications of AM fungi and ABA on plant growth, Zn accumulation, endogenous ABA contents, proline metabolism, and the oxidative injury of black locust (Robinia pseudoacacia L.) exposed to excess Zn stress. The results suggested that exogenously applied ABA positively enhanced AM colonization, and that the growth of plants only with AM fungi was improved by ABA application. Under Zn stress, AM inoculation and ABA application increased the ABA content in the root/leaf (increased by 48–172% and 92%, respectively) and Zn content in the root/shoot (increased by 63–152% and 61%, respectively) in AM plants, but no similar trends were observed in NM plants. Additionally, exogenous ABA addition increased the proline contents of NM roots concomitantly with the activities of the related synthases, whereas it reduced the proline contents and the activity of Δ1-pyrroline-5-carboxylate synthetase in AM roots. Under Zn stress, AM inoculation and ABA application decreased H2O2 contents and the production rate of O2, to varying degrees. Furthermore, in the roots exposed to Zn stress, AM inoculation augmented the activities of SOD, CAT, POD and APX, and exogenously applied ABA increased the activities of SOD and POD. Overall, AM inoculation combined with ABA application might be beneficial to the survival of black locust under Zn stress by improving AM symbiosis, inhibiting the transport of Zn from the roots to the shoots, increasing the distribution of ABA in roots, and stimulating antioxidant defense systems.

scholarly journals Biocontrol of Sclerotium rolfsii in Groundnut by Using Microbial Inoculants

2017 ◽  
Vol 9 (1) ◽  
pp. 124-130 ◽  
Author(s):  
Khirood DOLEY ◽  
Mayura DUDHANE ◽  
Mahesh BORDE
Keyword(s):  
Mycorrhizal Fungi ◽  
Antioxidant Activities ◽  
Sclerotium Rolfsii ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Stem Rot ◽  
Mycorrhizal Dependency ◽  
Trichoderma Species ◽  
Arachis Hypogaea L ◽  
Microbial Strains

Sclerotium rolfsii (Sacc.) is the causal agent of stem-rot in groundnut (Arachis hypogaea L.)crop. With the increase in demand for the groundnut, control of stem-rot efficiently by microbial strains is fast becoming inevitable as the conventional system of chemicals is degrading our ecosystem. This investigation here emphasizes on inoculation of arbuscular mycorrhizal fungi (AMF) and Trichoderma species for growth achievement and disease control. The present investigation showed that these microbial strains were found to be worth applying as they stimulated growth and decreased harmful effects of S. rolfsii (cv. ‘Western-51’). The increased biochemical parameters and antioxidant activities also indicated their defence related activities in groundnut plants. In spite of positive attributes meted out by these microbial strains towards groundnut crop, the interaction among AM fungi and Trichoderma species seemed to be less co-operative between each other which were noted when mycorrhizal dependency and percent root colonization were observed. However, in summary more practical application of low-input AM fungi along with Trichoderma species may be needed for the advancement of modern agricultural systems.

Sign in / Sign up

Export Citation Format

Share Document