Removal of gossypol from cottonseed by solvent extraction procedures

1952 ◽  
Vol 29 (8) ◽  
pp. 339-341 ◽  
Author(s):  
J. M. Dechary ◽  
R. P. Kupperman ◽  
F. H. Thurber ◽  
A. M. Altschul
Radiocarbon ◽  
2005 ◽  
Vol 47 (3) ◽  
pp. 425-432 ◽  
Author(s):  
Naohiko Ohkouchi ◽  
Timothy I Eglinton ◽  
Konrad A Hughen ◽  
Ellen Roosen ◽  
Lloyd D Keigwin

As a result of the growing use of multiple geochemical proxies to reconstruct ocean and climate changes in the past, there is an increasing need to establish temporal relationships between proxies derived from the same marine sediment record and ideally from the same core sections. Coupled proxy records of surface ocean properties, such as those based on lipid biomarkers (e.g. alkenone-derived sea surface temperature) and planktonic foraminiferal carbonate (oxygen isotopes), are a key example. Here, we assess whether 2 different solvent extraction procedures used for isolation of molecular biomarkers influence the radiocarbon contents of planktonic foraminiferal carbonate recovered from the corresponding residues of Bermuda Rise and Cariaco Basin sediments. Although minor Δ14C differences were observed between solvent-extracted and unextracted samples, no substantial or systematic offsets were evident. Overall, these data suggest that, in a practical sense, foraminiferal shells from a solvent-extracted residue can be reliably used for 14C dating to determine the age of sediment deposition and to examine age relationships with other sedimentary constituents (e.g. alkenones).


1972 ◽  
Vol 18 (5) ◽  
pp. 462-470 ◽  
Author(s):  
C Rimington ◽  
W G Sears ◽  
L Eales

Abstract The urinary porphyrin excretion of the patient (Part I) has been examined. Methods for determination of the various porphyrin fractions have also been compared and critically assessed. A thin-layer chromatographic technique was found to be rapid, discriminating, and precise. Of solvent-extraction procedures, that of Rimington and Benson for the determination of urinary "X" porphyrins gives results that compare well with those obtained by the quantitative chromatographic procedure. The patient excreted relatively large amounts of uroporphyrin, heptacarboxylic porphyrin, and "X" porphyrins. In addition, the hydroxycoproporphyrins 1 and 2 of Elder were present in the ether-soluble fraction of her urine in relatively large quantities during the period when the patient was severely ill. Her fecal porphyrins were not markedly increased. The porphyria exhibited by this case of Felty’s syndrome resembled the characteristic pattern of symptomatic porphyria, usually associated with alcoholism, but in this case other factors must be considered because this patient was a life-long abstainer from alcohol.


2019 ◽  
Vol 33 (9) ◽  
pp. 8145-8150
Author(s):  
Shaoqing Guo ◽  
Linchao Zhang ◽  
Yi Dong ◽  
Libing Gao ◽  
Yanzhi Cao ◽  
...  

1978 ◽  
Vol 176 (3) ◽  
pp. 919-926 ◽  
Author(s):  
R J Burgess ◽  
J H Walker ◽  
R J Mayer

1. The double-isotope concept [Arias, Doyle & Schimke (1969) J. Biol. Chem. 224, 3303–3315] for the measurement of protein turnover was used to estimate the turnover of proteins in subcellular and submitochondrial fractions prepared from rat liver. 2. Double-isotope experiments with [3H]leucine as first precursor and [14C]leucine as second precursor were used to measure the turnover rates of proteins in subcellular and submitochondrial fractions. Solvent extraction procedures designed to remove lipids and nucleic acids from trichloroacetic acid precipitates only changed the isotope ratio of the microsomal fraction. It was not possible to measure turnover of proteins in mitochondrial and submitochondrial fractions with these precursors. 3. Double-isotope experiments were designed to minimize first-precursor reutilization by employing NaH14CO3. [3H]Arginine was used as second precursor. The turnover rates of protein in subcellular and submitochondrial fractions was measured. Solvent extraction procedures designed to remove lipids and nucleic acids showed changes in the isotope ratio for all subcellular fractions, especially in microsomal and detergent-soluble mitochondrial fractions. Isotope ratios of precipitates after solvent extraction indicate that, whereas considerable heterogeneity exists for the average rates of protein turnover in subcellular fractions, little heterogeneity is observed in the average rates of protein turnover in submitochondrial fractions.


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