Terminally differentiating epithelial tissues in primary explant culture: A model of growth and development

1990 ◽  
Vol 26 (6) ◽  
pp. 543-553 ◽  
Author(s):  
Donald J. Merchant
Keyword(s):  
Growth And Development ◽  
Explant Culture ◽  
Epithelial Tissues ◽  
Primary Explant

A sequential cell kinetic study of meningioma cells in primary explant culture using bromodeoxyuridine

1993 ◽  
Vol 16 (2) ◽  
pp. 117-123
Author(s):  
Kusum Kharbanda ◽  
Asis K. Karak ◽  
Chitra Sarkar ◽  
Amit K. Dinda ◽  
Meera Mathur ◽  
...  
Keyword(s):  
Kinetic Study ◽  
Explant Culture ◽  
Cell Kinetic ◽  
Primary Explant

Primary explant culture: An in vitro model of the human prostate

The Prostate ◽  
1983 ◽  
Vol 4 (5) ◽  
pp. 523-542 ◽  
Author(s):  
Donald J. Merchant ◽  
S. M. Clarke ◽  
K. Ives ◽  
S. Harris
Keyword(s):  
In Vitro Model ◽  
Explant Culture ◽  
Human Prostate ◽  
Primary Explant ◽  
Vitro Model

scholarly journals Primary explant culture and collagen I substrate enhances corneal endothelial cell morphology

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Judith Zavala ◽  
María-Dolores Montalvo-Parra ◽  
Guillermo-Isaac Guerrero-Ramírez ◽  
Carlos-Alberto Rodríguez-Barrientos ◽  
Victor Treviño ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Morphology ◽  
Explant Culture ◽  
Corneal Endothelial Cell ◽  
Collagen I ◽  
Primary Explant

Salivary epithelial cells in primary culture: characterization of their growth and functional properties

1976 ◽  
Vol 20 (1) ◽  
pp. 149-165 ◽  
Author(s):  
C.B. Wigley ◽  
L.M. Franks
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Initial Period ◽  
Explant Culture ◽  
Submandibular Salivary Gland ◽  
Salivary Gland Cells ◽  
Primary Explant

Mouse submandibular salivary gland cells were grown in primary explant culture. After an initial period of degeneration within the explant, surviving epithelial cells proliferated rapidly and duct-like structures recolonized the explant. Autoradiographic studies showed that a peak of DNA synthesis occurred after 4 days in vitro and that proliferation was enhanced by insulin and hydrocortisone. These cells retained specialized secretory function (protease activity) for at least 2 weeks in vitro. This enzyme is a differentiated product of granular tubule cells in vivo. Between 6 and 10 days, explants attached to the substrate. An outgrowth developed, consisting largely of ultrastructurally identifiable epithelial cells which formed pseudoglandular structures in the monolayer. Epithelium survived for over 6 months in primary culture but could not be serially transferred. Secondary cultures were rapidly overgrown by mesenchymal cells.

An ultrastructural study of vegetative incompatibility in plants

1978 ◽  
Vol 36 (2) ◽  
pp. 436-437
Author(s):  
Randy Moore
Keyword(s):  
Ultrastructural Study ◽  
Cell Walls ◽  
Growth And Development ◽  
Solanum Pennellii ◽  
Initial Product ◽  
Basic Aspect ◽  
Tissue Interactions ◽  
Graft Interface ◽  
Antigen Antibody ◽  
Standard Fixation

Cell and tissue interactions are a basic aspect of eukaryotic growth and development. While cell-to-cell interactions involving recognition and incompatibility have been studied extensively in animals, there is no known antigen-antibody reaction in plants and the recognition mechanisms operating in plant grafts have been virtually neglected.An ultrastructural study of the Sedum telephoides/Solanum pennellii graft was undertaken to define possible mechanisms of plant graft incompatibility. Grafts were surgically dissected from greenhouse grown plants at various times over 1-4 weeks and prepared for EM employing variations in the standard fixation and embedding procedure. Stock and scion adhere within 6 days after grafting. Following progressive cell senescence in both Sedum and Solanum, the graft interface appears as a band of 8-11 crushed cells after 2 weeks (Fig. 1, I). Trapped between the buckled cell walls are densely staining cytoplasmic remnants and residual starch grains, an initial product of wound reactions in plants.

Identification of calcium oxalate crystals in dried or fixed tobacco leaf

1984 ◽  
Vol 42 ◽  
pp. 288-289
Author(s):  
Vicki L. Baliga ◽  
Mary Ellen Counts
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Calcium Oxalate ◽  
Growth And Development ◽  
Polarized Light ◽  
Calcium Oxalate Crystals ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron ◽  
Scanning Electron

Calcium is an important element in the growth and development of plants and one form of calcium is calcium oxalate. Calcium oxalate has been found in leaf seed, stem material plant tissue culture, fungi and lichen using one or more of the following methods—polarized light microscopy (PLM), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and x-ray diffraction.Two methods are presented here for qualitatively estimating calcium oxalate in dried or fixed tobacco (Nicotiana) leaf from different stalk positions using PLM. SEM, coupled with energy dispersive x-ray spectrometry (EDS), and powder x-ray diffraction were used to verify that the crystals observed in the dried leaf with PLM were calcium oxalate.

SEM study of the morphological effects of kinetin and zeatin on the growth and development of the apical meristem in wheat

1995 ◽  
Vol 53 ◽  
pp. 978-979
Author(s):  
G. M. Hutchins ◽  
J. S. Gardner
Keyword(s):  
Growth And Development ◽  
Furfuryl Alcohol ◽  
Apical Meristem ◽  
Plant Cells ◽  
Triticum Aestivum L ◽  
Morphological Development ◽  
Naturally Occurring ◽  
Chinese Spring Wheat ◽  
Sem Study ◽  
Moist Environment

Cytokinins are plant hormones that play a large and incompletely understood role in the life-cycle of plants. The goal of this study was to determine what roles cytokinins play in the morphological development of wheat. To achieve any real success in altering the development and growth of wheat, the cytokinins must be applied directly to the apical meristem, or spike of the plant. It is in this region that the plant cells are actively undergoing mitosis. Kinetin and Zeatin were the two cytokinins chosen for this experiment. Kinetin is an artificial hormone that was originally extracted from old or heated DNA. Kinetin is easily made from the reaction of adenine and furfuryl alcohol. Zeatin is a naturally occurring hormone found in corn, wheat, and many other plants.Chinese Spring Wheat (Triticum aestivum L.) was used for this experiment. Prior to planting, the seeds were germinated in a moist environment for 72 hours.

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