Expression of the human chondrocyte phenotype in vitro

1989 ◽  
Vol 25 (7) ◽  
pp. 659-668 ◽  
Author(s):  
Amy Lynn Aulthouse ◽  
Michael Beck ◽  
Edward Griffey ◽  
Julie Sanford ◽  
Karen Arden ◽  
...  
Keyword(s):  
Chondrocyte Phenotype ◽  
Human Chondrocyte

Nanog maintains human chondrocyte phenotype and function in vitro

2009 ◽  
Vol 28 (4) ◽  
pp. 516-521 ◽  
Author(s):  
Hongjun Zheng ◽  
Francoise Gourronc ◽  
Joseph A. Buckwalter ◽  
James A. Martin
Keyword(s):  
Chondrocyte Phenotype ◽  
Human Chondrocyte ◽  
And Function

ChemInform Abstract: Synthesis and in vitro Evaluation of 5-Arylidene-3-hydroxyalkyl-2-phenylimino-4-thiazolidinones (V) with Antidegenerative Activity on Human Chondrocyte Cultures.

ChemInform ◽  
2008 ◽  
Vol 39 (11) ◽  
Author(s):  
Rosaria Ottana ◽  
Rosanna Maccari ◽  
Rosella Ciurleo ◽  
Maria Gabriella Vigorita ◽  
Anna Maria Panico ◽  
...  
Keyword(s):  
In Vitro Evaluation ◽  
Human Chondrocyte ◽  
Chondrocyte Cultures

scholarly journals A Human Chondrocyte-Derived In Vitro Model of Alcohol-Induced and Steroid-Induced Femoral Head Necrosis

2018 ◽  
Vol 24 ◽  
pp. 539-547 ◽  
Author(s):  
Xiong Qin ◽  
Pan Jin ◽  
Tongmeng Jiang ◽  
Muyan Li ◽  
Jiachang Tan ◽  
...  
Keyword(s):  
Femoral Head ◽  
In Vitro Model ◽  
Femoral Head Necrosis ◽  
Human Chondrocyte ◽  
Vitro Model

scholarly journals Cycloastragenol as an Exogenous Enhancer of Chondrogenic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells. A Morphological Study

Cells ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 347 ◽  
Author(s):  
Marta Anna Szychlinska ◽  
Giovanna Calabrese ◽  
Silvia Ravalli ◽  
Nunziatina Laura Parrinello ◽  
Stefano Forte ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
Chondrogenic Differentiation ◽  
Hydrolysis Product ◽  
Cartilage Regeneration ◽  
Triterpenoid Saponin ◽  
Type I ◽  
Chondrocyte Phenotype

Stem cell therapy and tissue engineering represent a promising approach for cartilage regeneration. However, they present limits in terms of mechanical properties and premature de-differentiation of engineered cartilage. Cycloastragenol (CAG), a triterpenoid saponin compound and a hydrolysis product of the main ingredient in Astragalus membranaceous, has been explored for cartilage regeneration. The aim of this study was to investigate CAG’s ability to promote cell proliferation, maintain cells in their stable active phenotype, and support the production of cartilaginous extracellular matrix (ECM) in human adipose-derived mesenchymal stem cells (hAMSCs) in up to 28 days of three-dimensional (3D) chondrogenic culture. The hAMSC pellets were cultured in chondrogenic medium (CM) and in CM supplemented with CAG (CAG–CM) for 7, 14, 21, and 28 days. At each time-point, the pellets were harvested for histological (hematoxylin and eosin (H&E)), histochemical (Alcian-Blue) and immunohistochemical analysis (Type I, II, and X collagen, aggrecan, SOX9, lubricin). After excluding CAG’s cytotoxicity (MTT Assay), improved cell condensation, higher glycosaminoglycans (sGAG) content, and increased cell proliferation have been detected in CAG–CM pellets until 28 days of culture. Overall, CAG improved the chondrogenic differentiation of hAMSCs, maintaining stable the active chondrocyte phenotype in up to 28 days of 3D in vitro chondrogenic culture. It is proposed that CAG might have a beneficial impact on cartilage regeneration approaches.

Effects of intermittent hydrostatic pressure and BMP-2 on osteoarthritic human chondrocyte metabolism in vitro

2010 ◽  
Vol 29 (3) ◽  
pp. 361-368 ◽  
Author(s):  
R.L. Smith ◽  
D.P. Lindsey ◽  
L. Dhulipala ◽  
A.H.S. Harris ◽  
S.B. Goodman ◽  
...  
Keyword(s):  
Hydrostatic Pressure ◽  
Human Chondrocyte ◽  
Chondrocyte Metabolism

scholarly journals 269 POTENTIAL INVOLVEMENT OF GALECTIN-3 ON THE OSTEOARTHRITIC HUMAN CHONDROCYTE PHENOTYPE

2010 ◽  
Vol 18 ◽  
pp. S121
Author(s):  
P. Reboul ◽  
F. Cailotto ◽  
P. Netter ◽  
A. Bianchi ◽  
J.-Y. Jouzeau
Keyword(s):  
Chondrocyte Phenotype ◽  
Human Chondrocyte ◽  
Galectin 3

Synthesis and in vitro evaluation of 5-arylidene-3-hydroxyalkyl-2-phenylimino-4-thiazolidinones with antidegenerative activity on human chondrocyte cultures

2007 ◽  
Vol 15 (24) ◽  
pp. 7618-7625 ◽  
Author(s):  
Rosaria Ottanà ◽  
Rosanna Maccari ◽  
Rosella Ciurleo ◽  
Maria Gabriella Vigorita ◽  
Anna Maria Panico ◽  
...  
Keyword(s):  
In Vitro Evaluation ◽  
Human Chondrocyte ◽  
Chondrocyte Cultures

scholarly journals Macroscopical, Histological, and In Vitro Characterization of Nonosteoarthritic Versus Osteoarthritic Hip Joint Cartilage

2016 ◽  
Vol 9 ◽  
pp. CMAMD.S29844
Author(s):  
Jessica Badendick ◽  
Owen Godkin ◽  
Benjamin Kohl ◽  
Carola Meier ◽  
Michal Jagielski ◽  
...  
Keyword(s):  
Complement Regulatory Proteins ◽  
Chondrocyte Phenotype ◽  
Chondrocyte Culture ◽  
In Vitro Characterization ◽  
Femoral Head Cartilage ◽  
Culture Characteristics ◽  
Histological Results ◽  
Cultured Chondrocytes

Osteoarthritis (OA) might affect chondrocyte culture characteristics and complement expression. Therefore, this study addressed the interrelation between macroscopical and microscopical structure, complement expression, and chondrocyte culture characteristics in non-OA and OA cartilage. Femoral head cartilage samples harvested from patients with femoral neck fractures (FNFs) and OA were analyzed for macroscopical alterations using an in-house scoring system, graded histologically (Mankin score), and immunolabeled for complement regulatory proteins (CRPs) and receptors. Morphology of monolayer cultured chondrocytes isolated from a subset of samples was assessed. The macroscopical score distinguished the FNF and OA cartilage samples and correlated significantly with the histological results. Chondrocyte phenotype from FNF or OA cartilage differed. Complement receptor C5aR, CRPs CD55 and CD59, and weakly receptor C3AR were detected in the investigated FNF and OA cartilage, except for CD46, which was detected in only two of the five investigated donors. The in-house score also allows inexperienced observers to distinguish non-OA and OA cartilage for experimental purposes.

Sign in / Sign up

Export Citation Format

Share Document