Characteristics and aldehyde dehydrogenase activity of four rat hepatoma cell lines produced by diethylnitrosamine-phenobarbital treatment

1986 ◽  
Vol 22 (5) ◽  
pp. 263-272 ◽  
Author(s):  
Kwang-Huei Lin ◽  
Mark F. Leach ◽  
Alvin L. Winters ◽  
Ronald Lindahl
Keyword(s):  
Dehydrogenase Activity ◽  
Cell Lines ◽  
Aldehyde Dehydrogenase ◽  
Hepatoma Cell ◽  
Hepatoma Cell Lines ◽  
Rat Hepatoma ◽  
Aldehyde Dehydrogenase Activity

Increase in class 2 aldehyde dehydrogenase expression by arachidonic acid in rat hepatoma cells

2001 ◽  
Vol 357 (3) ◽  
pp. 811-818 ◽  
Author(s):  
Rosa A. CANUTO ◽  
Margherita FERRO ◽  
Raffaella A. SALVO ◽  
Anna M. BASSI ◽  
Antonella TROMBETTA ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Arachidonic Acid ◽  
Cell Growth ◽  
Cell Lines ◽  
Aldehyde Dehydrogenase ◽  
Hepatoma Cell ◽  
Hepatoma Cells ◽  
Hepatoma Cell Lines ◽  
Rat Hepatoma ◽  
Rat Hepatoma Cells

Aldehyde dehydrogenase (ALDH) is a family of several isoenzymes important in cell defence against both exogenous and endogenous aldehydes. Compared with normal hepatocytes, in rat hepatoma cells the following changes in the expression of ALDH occur: cytosolic class 3 ALDH expression appears and mitochondrial class 2 ALDH decreases. In parallel with these changes, a decrease in the polyunsaturated fatty acid content in membrane phospholipids occurs. In the present study we demonstrated that restoring the levels of arachidonic acid in 7777 and JM2 rat hepatoma cell lines to those seen in hepatocytes decreases hepatoma cell growth, and increases class 2 ALDH activity. This latter effect appears to be due to an increased gene transcription of class 2 ALDH. To account for this increase, we examined whether peroxisome-proliferator-activated receptors (PPARs) or lipid peroxidation were involved. We demonstrated a stimulation of PPAR expression, which is different in the two hepatoma cell lines: in the 7777 cell line, there was an increase in PPARα expression, whereas PPARγ expression increased in JM2 cells. We also found increased lipid peroxidation, but this increase became evident at a later stage when class 2 ALDH expression had already increased. In conclusion, arachidonic acid added to the culture medium of hepatoma cell lines is able to partially restore the normal phenotype of class 2 ALDH, in addition to a decrease in cell growth.

Effects of hexamethylene bisacetamide onα-fetoprotein, albumin, and transferrin production by two rat hepatoma cell lines

In Vitro ◽  
1982 ◽  
Vol 18 (2) ◽  
pp. 157-164 ◽  
Author(s):  
Everard H. Hughes ◽  
Herman A. J. Schut ◽  
Snorri S. Thorgeirsson
Keyword(s):  
Cell Lines ◽  
Hepatoma Cell ◽  
Hexamethylene Bisacetamide ◽  
Hepatoma Cell Lines ◽  
Rat Hepatoma

Cytotoxicity on Human Leukemic and Rat Hepatoma Cell Lines of Alkaloid Extracts ofPsychotria forsteriana

Planta Medica ◽  
1989 ◽  
Vol 55 (06) ◽  
pp. 567-568 ◽  
Author(s):  
Y. Adjibadé ◽  
B. Kuballa ◽  
P. Cabalion ◽  
M. Jung ◽  
J. Beck ◽  
...  
Keyword(s):  
Cell Lines ◽  
Hepatoma Cell ◽  
Hepatoma Cell Lines ◽  
Alkaloid Extracts ◽  
Rat Hepatoma
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