Effects of hexamethylene bisacetamide onα-fetoprotein, albumin, and transferrin production by two rat hepatoma cell lines

In Vitro ◽  
1982 ◽  
Vol 18 (2) ◽  
pp. 157-164 ◽  
Author(s):  
Everard H. Hughes ◽  
Herman A. J. Schut ◽  
Snorri S. Thorgeirsson
Keyword(s):  
Cell Lines ◽  
Hepatoma Cell ◽  
Hexamethylene Bisacetamide ◽  
Hepatoma Cell Lines ◽  
Rat Hepatoma

Cytotoxicity on Human Leukemic and Rat Hepatoma Cell Lines of Alkaloid Extracts ofPsychotria forsteriana

Planta Medica ◽  
1989 ◽  
Vol 55 (06) ◽  
pp. 567-568 ◽  
Author(s):  
Y. Adjibadé ◽  
B. Kuballa ◽  
P. Cabalion ◽  
M. Jung ◽  
J. Beck ◽  
...  
Keyword(s):  
Cell Lines ◽  
Hepatoma Cell ◽  
Hepatoma Cell Lines ◽  
Alkaloid Extracts ◽  
Rat Hepatoma

Molecular cloning of cDNA for rat argininosuccinate lyase and its expression in rat hepatoma cell lines

1986 ◽  
Vol 6 (5) ◽  
pp. 1722-1728
Author(s):  
M A Lambert ◽  
L R Simard ◽  
P N Ray ◽  
R R McInnes
Keyword(s):  
Cell Lines ◽  
Rat Liver ◽  
Transcriptional Activity ◽  
Hepatoma Cell ◽  
Cdna Clones ◽  
Argininosuccinate Lyase ◽  
Hepatoma Cell Lines ◽  
Liver Cdna Library ◽  
Nuclear Processing ◽  
Rat Hepatoma

Using antibody and plaque hybridization screening, we isolated rat argininosuccinate lyase (AS lyase) cDNA clones from a liver cDNA library prepared in the phage expression vector lambda gt11. Five overlapping cDNAs covering 1.7 kilobases of the estimated 2.0-kilobase AS lyase mRNA were characterized and confirmed as AS lyase sequences by hybrid selection. We examined the differential expression of AS lyase in rat liver and four rat hepatoma cell lines (7800C1, H4, HTC, and MH1C1). These cells exhibited a 60-fold range of AS lyase enzyme activity, with a direct correlation between activity, amount of AS lyase immunoreactive protein, and quantity of specific AS lyase mRNA. These observations suggest that the differences in AS lyase expression between rat liver and the hepatoma cell lines result from variations in AS lyase transcriptional activity or alterations in nuclear processing of AS lyase RNA.

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