Factors controlling embryonic heart cell proliferation in serum-free synthetic media

1985 ◽  
Vol 21 (10) ◽  
pp. 553-562 ◽  
Author(s):  
A. C. Nag ◽  
M. Ingland ◽  
M. Cheng
Keyword(s):  
Cell Proliferation ◽  
Embryonic Heart ◽  
Heart Cell ◽  
Serum Free ◽  
Synthetic Media

An embryonic heart cell line is susceptible to dengue virus infection

2015 ◽  
Vol 198 ◽  
pp. 53-58 ◽  
Author(s):  
Antonio H. Angel-Ambrocio ◽  
Rubén Soto-Acosta ◽  
Eshwar R. Tammineni ◽  
Elba D. Carrillo ◽  
Patricia Bautista-Carbajal ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Cell Line ◽  
Embryonic Heart ◽  
Heart Cell ◽  
Dengue Virus Infection

CARBOHYDRATE UTILIZATION BY CHICK EMBRYONIC HEART CULTURES

1960 ◽  
Vol 38 (1) ◽  
pp. 69-78 ◽  
Author(s):  
Joseph F. Morgan ◽  
Helen J. Morton
Keyword(s):  
Embryonic Heart ◽  
Serum Enzymes ◽  
Cell Multiplication ◽  
Complete Medium ◽  
Malignant Cells ◽  
Carbohydrate Utilization ◽  
Physiological Measurement ◽  
Normal Tissues ◽  
Heart Cultures ◽  
Synthetic Media

Freshly explanted chick embryonic heart fragments were cultivated in completely synthetic media. Survival of such cultures in the complete medium, containing glucose, was established at approximately 35 to 40 days, while in the absence of carbohydrate the cultures died within 3 to 5 days. Survival was considered to be a more physiological measurement than rapid cell multiplication for normal tissues and was adopted as the criterion for all experiments reported. Fifty-two compounds were tested for their ability to replace glucose, as the sole carbohydrate, in this system. Of these, seven (mannose, fructose, galactose, β-glucose, maltose, glucose-1-phosphate, and glucose-6-phosphate) replaced glucose completely. Five others (sorbitol, alpha-methyl-D-glucoside, turanose, dextrin, and fructose-6-phosphate) were partially active. The remainder were negative. Comparison is made of the present results with those obtained by other workers using malignant cells in the presence of serum–enzymes. The present results suggest that the ability to replace glucose decreases progressively as compounds down the Embden–Meyerhof pathway are tested.

scholarly journals Polyamine starvation causes accumulation of cadaverine and its derivatives in a polyamine-dependent strain of Chinese-hamster ovary cells

1983 ◽  
Vol 210 (3) ◽  
pp. 945-948 ◽  
Author(s):  
E Hölttä ◽  
P Pohjanpelto
Keyword(s):  
Cell Proliferation ◽  
Ornithine Decarboxylase ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Specific Inhibitor ◽  
Ovary Cells ◽  
Serum Free ◽  
Dependent Strain

Starvation of the polyamine-dependent Chinese-hamster ovary cells for ornithine or ornithine-derived polyamines in serum-free culture resulted in the formation of cadaverine and its aminopropyl derivatives, N-(3-aminopropyl)cadaverine and NN'-bis(3-aminopropyl)cadaverine. The synthesis of these unusual amines was inhibited by treatment of the cells with DL-2-difluoromethylornithine, a specific inhibitor of ornithine decarboxylase (EC 4.1.1.17). In the absence of ornithine (the normal substrate), ornithine decarboxylase thus appeared to catalyse the decarboxylation of lysine to cadaverine. Cell proliferation was markedly inhibited by ornithine deprivation of the cells, and further depressed by exposure of the cultures to difluoromethylornithine.

STUDIES ON THE SULPHUR METABOLISM OF TISSUES CULTIVATED IN VITRO: III. INTERRELATIONSHIPS BETWEEN CYSTEINE, METHIONINE, CYSTEAMINE, AND CYSTAMINE

1957 ◽  
Vol 35 (10) ◽  
pp. 785-794 ◽  
Author(s):  
Joseph F. Morgan ◽  
Helen J. Morton
Keyword(s):  
Amino Acid ◽  
Embryonic Heart ◽  
Tissue Cultures ◽  
Sulphur Metabolism ◽  
Amino Acid Requirement ◽  
Essential Requirement ◽  
Sulphur Amino Acid ◽  
Synthetic Media

Previous studies on the sulphur amino acid requirement of freshly-explanted chick embryonic heart tissues cultivated in vitro in completely synthetic media have been extended. The essential requirement for L-cystine has been found to be replaceable by L- but not by D-cysteine. In the presence of L-cysteine, the supplementary methionine requirement was found to be satisfied equally by the L- or D-isomer. Of eight derivatives and metabolites of cysteine studied, only cysteamine showed any significant ability to replace cysteine or cystine. The activity of cysteamine was exhibited only in the presence of methionine. High levels of cysteamine were found to be strongly toxic and the toxicity could be reversed specifically by L-cysteine. Significantly less reversal of the cysteamine toxicity was effected by L-cystine, while other sulphydryl and disulphide compounds were ineffective. Cystamine was also found to be toxic to the tissue cultures and its toxicity could be reversed completely by L-cysteine and incompletely by L-cystine.

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