A serum-free, chemically-defined medium for function and growth of primary neonatal rat heart cell cultures

In Vitro ◽  
1983 ◽  
Vol 19 (6) ◽  
pp. 471-478 ◽  
Author(s):  
S. N. W. Mohamed ◽  
R. Holmes ◽  
C. R. Hartzell
Keyword(s):  
Cell Cultures ◽  
Rat Heart ◽  
Defined Medium ◽  
Neonatal Rat ◽  
Heart Cell ◽  
Chemically Defined Medium ◽  
Serum Free ◽  
Neonatal Rat Heart

Lipid peroxidation in neonatal rat heart cell cultures: effects of cumene hydroperoxide

1986 ◽  
Vol 20 (1) ◽  
pp. 20-25 ◽  
Author(s):  
A. VAN DER LAARSE ◽  
R. H M JULICHER ◽  
L. STERRENBERG ◽  
C. H F B. VAN TRESLONG
Keyword(s):  
Lipid Peroxidation ◽  
Cell Cultures ◽  
Rat Heart ◽  
Cumene Hydroperoxide ◽  
Neonatal Rat ◽  
Heart Cell ◽  
Neonatal Rat Heart

Anoxia in neonatal rat heart cell cultures

1984 ◽  
pp. 137-142
Author(s):  
A. Van der Laarse ◽  
J. C. Altona ◽  
C. H. F. Bloys van Treslong
Keyword(s):  
Cell Cultures ◽  
Rat Heart ◽  
Neonatal Rat ◽  
Heart Cell ◽  
Neonatal Rat Heart

Release of compartment-specific enzymes from neonatal rat heart cell cultures during anoxia and reoxygenation

1984 ◽  
Vol 18 (2) ◽  
pp. 99-106 ◽  
Author(s):  
J C ALTONA ◽  
A VAN DER LAARSE ◽  
C H F B. VAN TRESLONG
Keyword(s):  
Cell Cultures ◽  
Rat Heart ◽  
Neonatal Rat ◽  
Heart Cell ◽  
Neonatal Rat Heart

scholarly journals Adenosine formation and release from neonatal-rat heart cells in culture

1985 ◽  
Vol 229 (3) ◽  
pp. 799-805 ◽  
Author(s):  
P Meghji ◽  
C A Holmquist ◽  
A C Newby
Keyword(s):  
Rat Heart ◽  
Neonatal Rat ◽  
Heart Cell ◽  
Heart Cells ◽  
Dependent Manner ◽  
Nucleoside Transporter ◽  
Concentration Dependent Manner ◽  
Cells In Culture ◽  
Neonatal Rat Heart ◽  
Rat Heart Cells

The incorporation of [3H]adenosine (10 microM) into neonatal-rat heart cell nucleotides was inhibited in a concentration-dependent manner, such that 50% inhibition was obtained with 0.75 microM-dipyridamole, 0.26 microM-hexobendine or 0.22 microM-dilazep. Adenosine formation was accelerated 2.5-fold to 2.1 +/- 0.3 nmol/10(7) cells in 10 min when cells were incubated with a combination of 30 mM-2-deoxyglucose and 2 micrograms of oligomycin/ml. Of the newly formed adenosine, 6 +/- 2% was in the cells. Dipyridamole, hexobendine or dilazep (10 microM) increased the amount of adenosine in the cells and decreased that in the medium such that 45-50% of the newly formed adenosine was in the cells. Antibodies which inhibited ecto-5'-nucleotidase by 98.7 +/- 0.3% did not alter the rate of adenosine formation or its distribution between cells and medium. We conclude that adenosine was formed in the cytoplasm during catabolism of cellular ATP and was released via the dipyridamole-sensitive symmetric nucleoside transporter.

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