Establishment and morphologic characterization of normal human endometrium in vitro

In Vitro ◽  
1984 ◽  
Vol 20 (6) ◽  
pp. 451-462 ◽  
Author(s):  
G. M. Centola ◽  
M. Cisar ◽  
D. R. Knab
Keyword(s):  
Human Endometrium ◽  
Normal Human

scholarly journals Construction and Characterization of a Pseudomonas aeruginosa Mucoid Exopolysaccharide-Alginate Conjugate Vaccine

2003 ◽  
Vol 71 (7) ◽  
pp. 3875-3884 ◽  
Author(s):  
Christian Theilacker ◽  
Fadie T. Coleman ◽  
Simone Mueschenborn ◽  
Nicolas Llosa ◽  
Martha Grout ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Conjugate Vaccine ◽  
Significant Proportion ◽  
Vaccine Trial ◽  
Keyhole Limpet Hemocyanin ◽  
Opsonic Activity ◽  
Human Sera ◽  
Normal Human

ABSTRACT Deterioration of lung function in patients with cystic fibrosis (CF) is closely associated with chronic pulmonary infection with mucoid Pseudomonas aeruginosa. The mucoid exopolysaccharide (MEP) from P. aeruginosa has been shown to induce opsonic antibodies in mice that are protective against this chronic infection. MEP-specific opsonic antibodies are also commonly found in the sera of older CF patients lacking detectable P. aeruginosa infection. When used in a human vaccine trial, however, MEP only minimally induced opsonic antibodies. To evaluate whether conjugation of MEP to a carrier protein could improve its immunogenicity, we bound thiolated MEP to keyhole limpet hemocyanin (KLH) by using succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC) as a linker. In contrast to the native MEP polymer, the MEP-KLH conjugate vaccine induced high titers of MEP-specific immunoglobulin G (IgG) in C3H-HeN mice and in a rabbit. Sera from mice immunized with MEP-KLH conjugate, but not from animals immunized with comparable doses of native MEP, demonstrated opsonic killing activity. Vaccination with MEP-KLH conjugate induced opsonic antibodies broadly cross-reactive to heterologous mucoid strains of P. aeruginosa. Preexisting nonopsonic antibodies to MEP are found in normal human sera, including young CF patients, and their presence impedes the induction of opsonic antibodies. Induction of nonopsonic antibodies by either intraperitoneal injection of MEP or injection or feeding of the cross-reactive antigen, seaweed alginate, reduced the level of overall IgG elicited by follow-up immunization with the MEP-KLH conjugate. However, the opsonic activity was lower only in the sera of MEP-KLH conjugate-immunized mice with preexisting antibodies induced by MEP but not with antibodies induced by seaweed alginate. Immunization with MEP-KLH elicited a significant proportion of antibodies specific to epitopes involving O-acetate residues, and this subpopulation of antibodies mediated opsonic killing of mucoid P. aeruginosa in vitro. These results indicate that conjugation of MEP to KLH significantly enhances its immunogenicity and the elicitation of opsonic antibodies in mice and rabbits, that the conjugate induces opsonic antibodies in the presence of preexisting nonopsonic antibodies, and that opsonic antibodies to MEP are directed at epitopes that include acetate residues on the uronic acid polymer.

Epithelial cells from normal human endometrium express a tumor-associated glycoprotein (TAG-72) epitope in vitro

1991 ◽  
Vol 35 (2) ◽  
pp. 195-195
Author(s):  
KG Osteen ◽  
TL Anderson ◽  
JT Hargrove ◽  
GA Hill ◽  
F Gorstein
Keyword(s):  
Epithelial Cells ◽  
Human Endometrium ◽  
Normal Human

Characterization of the Nuclear Progesterone Receptor in Normal Human Endometrium after Progesterone Injection

1987 ◽  
Vol 23 (1) ◽  
pp. 48-53
Author(s):  
I.G. Gorodeski ◽  
C.M. Bahary ◽  
R. Beery ◽  
B. Lunenfeld ◽  
A. Geier
Keyword(s):  
Progesterone Receptor ◽  
Human Endometrium ◽  
Normal Human ◽  
Nuclear Progesterone Receptor

Comparison of raloxifene effect on the growth and angiogenesis of human endometrium of healthy and endometriosis subjects: An in vitro three-dimensional tissue culture model

2021 ◽  
pp. 228402652110183
Author(s):  
Parviz Ranjbarvan ◽  
Fatemeh Khazaei ◽  
Farzaneh Chobsaz ◽  
Mozafar Khazaei
Keyword(s):  
Tissue Culture ◽  
Three Dimensional ◽  
Tissue Growth ◽  
Human Endometrium ◽  
Selective Oestrogen Receptor Modulators ◽  
Culture Model ◽  
Tissue Culture Model ◽  
Normal Human ◽  
Growth Scores

Introduction: Raloxifene (Ral) is the oldest SERM (selective oestrogen receptor modulators) for treatment of breast cancer and osteoporosis. Its oestrogen-modulating effects have been shown in breast and uterus. Since there is little available data on direct Ral effect on the human endometrium, the aim of present study was to investigate the Ral effect on the growth and angiogenesis of the human endometrium of healthy and endometriosis subjects in an in vitro three-dimensional (3D) tissue culture model. Material and methods: Endometrial biopsies from healthy ( n = 9) and endometriosis ( n = 7) patients (endometriotic) were taken and were cut into 1 × 1 mm fragments and implanted between two layers of fibrin jell made by fibrinogen solution (3 mg/ml in medium 199+thrombin). Tissue cultures were performed in 24-wel culture plates. Each biopsy was divided into control wells which received M199 supplemented with FBS (5%) and experimental wells which received same media containing one of raloxifene doses (0.1, 1 and 10 μM). Endometrial tissues were photographed at the beginning and the end of the study period (21 days). Tissue growth and angiogenesis were determined by a scoring system. Results: In control (0), 0.1, 1 and 10 μM Ral, the growth score of normal human endometrial tissues were 1.99, 1.72, 1.53 and 1.12 ( p = 0.02) and angiogenesis percent were 29.6%, 31.28%, 33% and 11.5%. The Growth scores of the endometriotic endometrium were 1.92, 1.82, 1.92 and 1.1 ( p = 0.008) and angiogenesis percent were 36.6%, 16.6%, 44% and 12.5% respectively. Conclusion: Raloxifene showed a different dose dependent effect on endometrial and endometriotic tissue.

scholarly journals Purification and characterization of a novel monocyte chemotactic and activating factor produced by a human myelomonocytic cell line.

1989 ◽  
Vol 169 (4) ◽  
pp. 1485-1490 ◽  
Author(s):  
K Matsushima ◽  
C G Larsen ◽  
G C DuBois ◽  
J J Oppenheim
Keyword(s):  
Cell Line ◽  
Human Tumor ◽  
Heparin Binding ◽  
Purification And Characterization ◽  
Human Tumor Cell Lines ◽  
Growth Inhibitory ◽  
Sds Page ◽  
Normal Human

A novel basic heparin-binding monocyte chemotactic factor (MCF) was purified to homogeneity from the conditioned media of human myelomonocytic cell line THP-1 based on its in vitro monocyte chemotactic activity. The purified MCF was homogenous and estimated to be 15 kD on SDS-PAGE. Purified MCF stimulated normal human monocytes to be growth inhibitory in vitro at 2-3 d for several human tumor cell lines. This represents the first report of the identification and purification of a chemoattractant cytokine that also activates monocytes but is distinct from interferons and other known cytokines.

Epithelial cells from normal human endometrium express a tumor-associated glycoprotein (TAG-72) epitope in vitro

1990 ◽  
Vol 163 (2) ◽  
pp. 479-484 ◽  
Author(s):  
Kevin G. Osteen ◽  
Ted L. Anderson ◽  
Joel T. Hargrove ◽  
George A. Hill ◽  
Fred Gorstein
Keyword(s):  
Epithelial Cells ◽  
Human Endometrium ◽  
Normal Human

Characterization of Procoagulants Produced in Cell Cultures by Tests of Coagulation

1971 ◽  
Vol 26 (03) ◽  
pp. 493-502 ◽  
Author(s):  
L. R Zacharski ◽  
O. R Mcintyre
Keyword(s):  
Tissue Factor ◽  
Cell Cultures ◽  
Human Fibroblasts ◽  
Factor Vii ◽  
Coagulation Tests ◽  
Normal Human

SummaryA potent procoagulant is synthesized by normal human fibroblasts cultivated in vitro. Although evidence is presented indicating that phospholipid (capable of substituting for platelets) and tissue factor (capable of complexing factor VII) are present in homogenates of saline-washed fibroblast monolayers, these substances do not appear to account entirely for the rise in procoagulant levels observed. The behavior of this procoagulant as assessed by a variety of coagulation tests, suggests that the procoagulant has physiologic significance.

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