Growth and characterization of normal human urothelium in vitro

In Vitro ◽  
1983 ◽  
Vol 19 (4) ◽  
pp. 326-343 ◽  
Author(s):  
Catherine A. Reznikoff ◽  
Mark D. Johnson ◽  
Diane H. Norback ◽  
George T. Bryan
Keyword(s):  
Normal Human ◽  
Normal Human Urothelium

scholarly journals Construction and Characterization of a Pseudomonas aeruginosa Mucoid Exopolysaccharide-Alginate Conjugate Vaccine

2003 ◽  
Vol 71 (7) ◽  
pp. 3875-3884 ◽  
Author(s):  
Christian Theilacker ◽  
Fadie T. Coleman ◽  
Simone Mueschenborn ◽  
Nicolas Llosa ◽  
Martha Grout ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Conjugate Vaccine ◽  
Significant Proportion ◽  
Vaccine Trial ◽  
Keyhole Limpet Hemocyanin ◽  
Opsonic Activity ◽  
Human Sera ◽  
Normal Human

ABSTRACT Deterioration of lung function in patients with cystic fibrosis (CF) is closely associated with chronic pulmonary infection with mucoid Pseudomonas aeruginosa. The mucoid exopolysaccharide (MEP) from P. aeruginosa has been shown to induce opsonic antibodies in mice that are protective against this chronic infection. MEP-specific opsonic antibodies are also commonly found in the sera of older CF patients lacking detectable P. aeruginosa infection. When used in a human vaccine trial, however, MEP only minimally induced opsonic antibodies. To evaluate whether conjugation of MEP to a carrier protein could improve its immunogenicity, we bound thiolated MEP to keyhole limpet hemocyanin (KLH) by using succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC) as a linker. In contrast to the native MEP polymer, the MEP-KLH conjugate vaccine induced high titers of MEP-specific immunoglobulin G (IgG) in C3H-HeN mice and in a rabbit. Sera from mice immunized with MEP-KLH conjugate, but not from animals immunized with comparable doses of native MEP, demonstrated opsonic killing activity. Vaccination with MEP-KLH conjugate induced opsonic antibodies broadly cross-reactive to heterologous mucoid strains of P. aeruginosa. Preexisting nonopsonic antibodies to MEP are found in normal human sera, including young CF patients, and their presence impedes the induction of opsonic antibodies. Induction of nonopsonic antibodies by either intraperitoneal injection of MEP or injection or feeding of the cross-reactive antigen, seaweed alginate, reduced the level of overall IgG elicited by follow-up immunization with the MEP-KLH conjugate. However, the opsonic activity was lower only in the sera of MEP-KLH conjugate-immunized mice with preexisting antibodies induced by MEP but not with antibodies induced by seaweed alginate. Immunization with MEP-KLH elicited a significant proportion of antibodies specific to epitopes involving O-acetate residues, and this subpopulation of antibodies mediated opsonic killing of mucoid P. aeruginosa in vitro. These results indicate that conjugation of MEP to KLH significantly enhances its immunogenicity and the elicitation of opsonic antibodies in mice and rabbits, that the conjugate induces opsonic antibodies in the presence of preexisting nonopsonic antibodies, and that opsonic antibodies to MEP are directed at epitopes that include acetate residues on the uronic acid polymer.

A biomimetic tissue from cultured normal human urothelial cells: analysis of physiological function

2005 ◽  
Vol 289 (2) ◽  
pp. F459-F468 ◽  
Author(s):  
W. R. Cross ◽  
I. Eardley ◽  
H. J. Leese ◽  
J. Southgate
Keyword(s):  
Urothelial Cells ◽  
Functional Relationships ◽  
Basal Expression ◽  
Physiological Investigation ◽  
Cell Culture Systems ◽  
Diffusive Permeability ◽  
Normal Human ◽  
Sodium Ion Channels ◽  
Normal Human Urothelium

The urinary bladder and associated tract is lined by the urothelium. Once considered as just an impermeable epithelium, it is becoming evident that the urothelium not only functions as a volume-accommodating urinary barrier but has additional roles, including sensory signaling. Lack of access to normal human urothelium has hampered physiological investigation, and although cell culture systems have been developed, there has been a failure to demonstrate that normal human urothelial (NHU) cells grown in vitro retain the capacity to form a functional differentiated urothelium. The aim of this study was to develop a biomimetic human urothelium from NHU cell cultures. Urothelial cells isolated from normal human urothelium and serially propagated as monolayers in serum-free culture were homogeneous and adopted a proliferative, nondifferentiated phenotype. In the presence of serum and physiological concentrations of calcium, these cells could be reproducibly induced to form stratified urothelia consisting of basal, intermediate, and superficial cells, with differential expression of cytokeratins and superficial tight junctions. Functionally, the neotissues showed characteristics of native urothelium, including high transepithelial electrical resistance of >3,000 Ω·cm2, apical membrane-restricted amiloride-sensitive sodium ion channels, basal expression of Na+-K+-ATPase, and low diffusive permeability to urea, water, and dextran. This model represents major progress in developing a biomimetic human urothelial culture model to explore molecular and functional relationships in normal and dysfunctional bladder physiology.

scholarly journals Purification and characterization of a novel monocyte chemotactic and activating factor produced by a human myelomonocytic cell line.

1989 ◽  
Vol 169 (4) ◽  
pp. 1485-1490 ◽  
Author(s):  
K Matsushima ◽  
C G Larsen ◽  
G C DuBois ◽  
J J Oppenheim
Keyword(s):  
Cell Line ◽  
Human Tumor ◽  
Heparin Binding ◽  
Purification And Characterization ◽  
Human Tumor Cell Lines ◽  
Growth Inhibitory ◽  
Sds Page ◽  
Normal Human

A novel basic heparin-binding monocyte chemotactic factor (MCF) was purified to homogeneity from the conditioned media of human myelomonocytic cell line THP-1 based on its in vitro monocyte chemotactic activity. The purified MCF was homogenous and estimated to be 15 kD on SDS-PAGE. Purified MCF stimulated normal human monocytes to be growth inhibitory in vitro at 2-3 d for several human tumor cell lines. This represents the first report of the identification and purification of a chemoattractant cytokine that also activates monocytes but is distinct from interferons and other known cytokines.

Characterization of Procoagulants Produced in Cell Cultures by Tests of Coagulation

1971 ◽  
Vol 26 (03) ◽  
pp. 493-502 ◽  
Author(s):  
L. R Zacharski ◽  
O. R Mcintyre
Keyword(s):  
Tissue Factor ◽  
Cell Cultures ◽  
Human Fibroblasts ◽  
Factor Vii ◽  
Coagulation Tests ◽  
Normal Human

SummaryA potent procoagulant is synthesized by normal human fibroblasts cultivated in vitro. Although evidence is presented indicating that phospholipid (capable of substituting for platelets) and tissue factor (capable of complexing factor VII) are present in homogenates of saline-washed fibroblast monolayers, these substances do not appear to account entirely for the rise in procoagulant levels observed. The behavior of this procoagulant as assessed by a variety of coagulation tests, suggests that the procoagulant has physiologic significance.

The barrier properties of in vitro generated normal human urothelium

2002 ◽  
Vol 30 (1) ◽  
pp. A44-A44
Author(s):  
W.R. Cross ◽  
I. Eardley ◽  
H.J. Leese ◽  
J. Southgate
Keyword(s):  
Barrier Properties ◽  
Normal Human ◽  
Normal Human Urothelium

scholarly journals Cytotoxic Activity of Zinc Oxide Nanoparticles Using Zingiber officinale Rhizome Extracts Against Leukemia (K562) Cell Line

2021 ◽  
Vol 71 (1) ◽  
Author(s):  
Sayantani Pal ◽  
Tapas Kumar Pal ◽  
Shila Elizabeth Besra
Keyword(s):  
Zinc Oxide ◽  
Cytotoxic Activity ◽  
Zingiber Officinale ◽  
Medical Application ◽  
Significant Inhibition ◽  
Myelogenous Leukemia ◽  
Zno Nanoparticle ◽  
Normal Human

Zinc Oxide (ZnO) nanoparticle was synthesized using rhizome extracts of Zingiber officinale for in-vitro anti-cancer study. The characterization of nanoparticle was confirmed using ultraviolet spectroscopy and Fourier Transfer Infrared Spectroscopy (FTIR). The ZGNPE characterization also used the UV-Vis Spectrophotometer spectrum technique to measure the structural characterization of nanoparticles by determine the absorbance measurement. Cytotoxic activity of myelogenous leukemia cells (K562) and normal human embryonic Kidney (HEK293T) cells were performed by MTT assay. Treated with ZnO nanoparticle with Zingiber officinale extracts (ZGNPE) showed significant inhibition on the cancer cells whereas in normal cell showed insignificant toxicity. Thus, ZGNPE may be used as chemotherapy drug and an enormous medical application with less toxicity in future. Further mechanistic studies are in progress.

Establishment and morphologic characterization of normal human endometrium in vitro

In Vitro ◽  
1984 ◽  
Vol 20 (6) ◽  
pp. 451-462 ◽  
Author(s):  
G. M. Centola ◽  
M. Cisar ◽  
D. R. Knab
Keyword(s):  
Human Endometrium ◽  
Normal Human
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