Growth and ultrastructural characterization of proliferating human keratinocytes in vitro without added extrinsic factors

In Vitro ◽  
1980 ◽  
Vol 16 (1) ◽  
pp. 20-30 ◽  
Author(s):  
George E. Milo ◽  
G. Adolph Ackerman ◽  
Ingeborg Noyes
Keyword(s):  
Human Keratinocytes ◽  
Extrinsic Factors ◽  
Ultrastructural Characterization

scholarly journals Ultrastructural Characterization of Porcine Growing and In Vitro Matured Oocytes

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 664
Author(s):  
Michel Kere ◽  
Pan-Chen Liu ◽  
Yuh-Kun Chen ◽  
Pei-Chi Chao ◽  
Li-Kuang Tsai ◽  
...  
Keyword(s):  
Single Layer ◽  
Ultrastructural Changes ◽  
Cortical Granules ◽  
Oocyte Growth ◽  
Future Studies ◽  
Morphological Alterations ◽  
Ultrastructural Characterization ◽  
Subcellular Level

This study aimed to investigate ultrastructural changes of growing porcine oocytes and in vitro maturated oocytes. Light microscopy was used to characterize and localize the primordial, primary, secondary, and tertiary follicles. During oocyte growth and maturation, the morphology of mitochondria was roundish or ovoid in shape depending on the differentiation state, whereas their mean diameters oscillated between 0.5 and 0.7 µm, respectively, from primary and secondary follicles. Hooded mitochondria were found in the growing oocytes of the tertiary follicles. In addition to the pleomorphism of mitochondria, changes in the appearance of lipid droplets were also observed, along with the alignment of a single layer of cortical granules beneath the oolemma. In conclusion, our study is apparently the first report to portray morphological alterations of mitochondria that possess the hooded structure during the growth phase of porcine oocytes. The spatiotemporal and intrinsic changes during oogenesis/folliculogenesis are phenomena at the ultrastructural or subcellular level of porcine oocytes, highlighting an in-depth understanding of oocyte biology and impetus for future studies on practical mitochondrion replacement therapies for oocytes.

Ultrastructural characterization of murine limb buds after in vitro exposure to grepafloxacin and other fluoroquinolones

2001 ◽  
Vol 75 (11-12) ◽  
pp. 725-733 ◽  
Author(s):  
Mehdi Shakibaei ◽  
Irmela Baumann-Wilschke ◽  
Marcus Rücker ◽  
Ralf Stahlmann
Keyword(s):  
Limb Buds ◽  
Ultrastructural Characterization ◽  
In Vitro Exposure

An Ultrastructural Characterization of in Vitro-Assembled hnRNP C Protein-RNA Complexes

1995 ◽  
Vol 114 (2) ◽  
pp. 84-92 ◽  
Author(s):  
J.E. Rech ◽  
M.H. Huang ◽  
W.M. LeStourgeon ◽  
P.F. Flicker
Keyword(s):  
C Protein ◽  
Ultrastructural Characterization ◽  
Hnrnp C ◽  
Rna Complexes

scholarly journals Morphobiological, morphometric and ultrastructural characterization of sylvatic Trypanosoma cruzi isolates from Rio de Janeiro state, Brazil

2019 ◽  
Vol 79 (2) ◽  
pp. 294-303 ◽  
Author(s):  
C. Santos da Silva ◽  
A. L. Carbajal-de-la-Fuente ◽  
C. E. Almeida ◽  
T. C. M. Gonçalves ◽  
J. Reis dos Santos-Mallet
Keyword(s):  
Trypanosoma Cruzi ◽  
Rio De Janeiro ◽  
Natural Infection ◽  
Growth Curves ◽  
Maximum Growth ◽  
Ultrastructural Characterization ◽  
High Prevalence ◽  
Janeiro State

Abstract Triatoma vitticeps is a triatomine with geographic distribution restrict to Brazil, which exhibits high prevalence of Trypanosoma cruzi natural infection. Of special epidemiologic concern, this species often invades households in the states of Rio de Janeiro, Minas Gerais and Espírito Santo. The objective of this study was to evaluate morphological and ultrastructural parameters on three T. cruzi isolates obtained from wild T. vitticeps specimens. The growth and cell differentiation of the parasite was evaluated through epimastigote and trypomastigote forms obtained in the growth curves for three distinct isolates. The maximum growth showed differences at the 20th day of the curve. Our in vitro results show a heterogeneity, regarding these features for samples cultivated under the same conditions. Morphometric analyzes based on the shape of epimastigotes and trypomastigotes corroborated such differentiation. These results highlight the need of better understanding the meaning of this diversity under an eco-epidemiological perspective.

Ultrastructural characterization of the extravillous trophoblast (EVT) cells differentiated in vitro

Placenta ◽  
1992 ◽  
Vol 13 (4) ◽  
pp. A12
Author(s):  
Karen deMesy Jensen ◽  
Olga Genbacev ◽  
P.A. di Sant Agnese ◽  
Richard K. Miller
Keyword(s):  
Extravillous Trophoblast ◽  
Ultrastructural Characterization

scholarly journals Histochemical and ultrastructural characterization of subendothelial glycoprotein microfibrils interacting with platelets.

1982 ◽  
Vol 30 (1) ◽  
pp. 75-80 ◽  
Author(s):  
P Birembaut ◽  
Y J Legrand ◽  
J Bariety ◽  
R Bretton ◽  
F Fauvel ◽  
...  
Keyword(s):  
Concanavalin A ◽  
Ricinus Communis ◽  
Blood Platelets ◽  
Ruthenium Red ◽  
Fibrillar Collagen ◽  
Bacterial Collagenase ◽  
Ultrastructural Characterization ◽  
Human Blood Platelets

The interaction of human blood platelets with collagenase-treated rabbit subendothelium was studied by histochemical ultrastructural methods and by morphometric semi-quantitative analysis. Aortas were deendothelialized and incubated: 1) with a highly purified bacterial collagenase whose specificity was controlled; and 2) with the same collagenase followed by chymotrypsin. For histochemical studies, tannic acid, ruthenium red, and peroxidase-labeled Ricinus communis and concanavalin A were used. Electron microscopy showed that after digestion of fibrillar collagen by collagenase, adherent and aggregated platelets were observed on Ricinus communis-, concanavalin A-, and ruthenium red-positive glycoprotein microfibrils. After successive incubation with collagenase and chymotrypsin, the microfibrils disappeared. No platelets were observed on the remnant amorphous elastin. Morphometric analysis confirmed the interaction of platelets with collagenase-treated subendothelium. In addition, glycoproteins were extracted from collagenase-treated rabbit aortas using 5 M guanidine. Using an in vitro quantitative test, significant platelet adhesion to these glycoproteins was observed. Our results show an interaction between platelets and noncollagenic glycoprotein microfibrils.

scholarly journals In VitroandIn SituCharacterization of Fish Thymic Nurse Cells

1996 ◽  
Vol 5 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Emilio Flaño ◽  
Francisco Álvarez ◽  
Pilar López-Fierro ◽  
Blanca E. Razquin ◽  
Alberto J. Villena ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Indirect Evidence ◽  
Nurse Cells ◽  
Thymic Nurse Cells ◽  
Mhc Ii ◽  
Ultrastructural Characterization ◽  
Acidic Compartments

We present an enzyme- and immuno-cytochemical, and ultrastructural characterization of trout thymic nurse cells (TNCs). Our data suggest that isolated trout thymic multicellular complexes are epithelial cells with acidic compartments that may be involved in the processing of antigens and in the generation of the MHC-II proteins that these cell express, and also that isolated TNCs are theIn Vitroequivalent of the pale and intermediate electronlucent epithelial cells located in the inner zone of the trout thymus, constituting indirect evidence of the phylogenetical relationships of the inner zone of the teleost thymus with the thymic cortex of higher vertebrates.

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