Development of sertoli cell junctions in vitro—A freeze-fracture study

In Vitro ◽  
1978 ◽  
Vol 14 (11) ◽  
pp. 916-923 ◽  
Author(s):  
Rita Meyer ◽  
Zoltan Posalaky ◽  
Dennis McGinley
Keyword(s):  
Sertoli Cell ◽  
Freeze Fracture ◽  
Cell Junctions ◽  
Fracture Study

scholarly journals Perturbation of microRNA signalling by doxorubicin in spermatogonial, Leydig and Sertoli cell lines in vitro

2018 ◽  
Vol 7 (5) ◽  
pp. 760-770 ◽  
Author(s):  
Oluwajoba O. Akinjo ◽  
Timothy W. Gant ◽  
Emma L. Marczylo
Keyword(s):  
Sertoli Cell ◽  
Cell Lines ◽  
Cell Types ◽  
Cell Junctions ◽  
Testicular Toxicity ◽  
Testicular Cell

Doxorubicin-induced testicular toxicity involves perturbation of microRNAs within all three of the main testicular cell types, particularly those involved in germ–Sertoli and Sertoli–Sertoli cell junctions.

Freeze-fracture study of mammalian skin: melanocyte-keratinocyte interactions

1978 ◽  
Vol 36 (2) ◽  
pp. 652-653
Author(s):  
Raul I. Garcia ◽  
Evelyn A. Flynn ◽  
George Szabo
Keyword(s):  
Skin Color ◽  
Freeze Fracture ◽  
Culture Studies ◽  
Mammalian Skin ◽  
Lanthanum Tracer ◽  
Fracture Study ◽  
Thin Section Analysis ◽  
Section Analysis

The interactions of epidermal melanocytes(M) and keratinocytes(K) involve the unique process of melanosome transfer, from the melanin-synthesizing cell to the recipient, desquamative K, and are responsible for the skin color of man and other mammals. M-K interactions have been well studied in mammalian skin using thin section analysis, and there have been several freeze-fracture studies of skin. This study represents the first application of freeze-fracture to the study of M-K interactions and melanosome transfer. Several mechanisms of melanosome transfer have been proposed to explain the varied observations made in vivo and in vitro: melanosomes migrate from the M perikaryon peripherally into M dendrites, followed by cytophagocytosis by the K of the M dendrite tip containing melanosomes, formation of intercellular communicating channels between M and K through localized fusion of cell membranes with melanosome entry into K through this passage, release of melanosomes from M into the extracellular space(ECS) by exocytosis with subsequent phagocytosis by the K. Our own EM work using lanthanum tracer in skin in vivo has produced evidence supporting the second mechanism while cell culture studies suggest that a different mechanism may operate in vitro.

scholarly journals FSH regulates the formation of adherens junctions and ectoplasmic specialisations between rat Sertoli cells in vitro and in vivo

2006 ◽  
Vol 189 (2) ◽  
pp. 381-395 ◽  
Author(s):  
P Sluka ◽  
L O’Donnell ◽  
J R Bartles ◽  
P G Stanton
Keyword(s):  
Sertoli Cell ◽  
Sertoli Cells ◽  
Adherens Junctions ◽  
Adherens Junction ◽  
Cell Junctions ◽  
Junctional Complex ◽  
Intermediate Filament Protein ◽  
Adult Rat

Spermatogenesis is dependent on the ability of Sertoli cells to form mature junctions that maintain a unique environment within the seminiferous epithelium. Adjacent Sertoli cells form a junctional complex that includes classical adherens junctions and testis-specific ectoplasmic specialisations (ES). The regulation of inter-Sertoli cell junctions by the two main endocrine regulators of spermatogenesis, FSH and testosterone, is unclear. This study aimed to investigate the effects of FSH and testosterone on inter-Sertoli cell adherens junctions (as determined by immunolocalisation of cadherin, catenin and actin) and ES junctions (as determined by immunolocalisation of espin, actin and vinculin) in cultured immature Sertoli cells and GnRH-immunised adult rat testes given FSH or testosterone replacement in vivo. When hormones were absent in vitro, adherens junctions formed as discrete puncta between interdigitating, finger-like projections of Sertoli cells, but ES junctions were not present. The adherens junction puncta included actin filaments that were oriented perpendicularly to the Sertoli cell plasma membrane, but were not associated with the intermediate filament protein vimentin. When FSH was added in vitro, ES junctions formed, and adjacent adherens junction puncta fused into extensive adherens junction belts. After hormone suppression in vivo, ES junctions were absent, while FSH replacement restored ES junctions, as confirmed by electron microscopy and confocal analysis of ES-associated proteins. Testosterone alone did not affect adherens junctions or ES in vitro or in vivo. We conclude that FSH can regulate the formation of ES junctions and stimulate the organisation and orientation of extensive adherens junctions in Sertoli cells.

Cell junctions in amphioxus (Cephalochordata): A thin section and freeze-fracture study

1987 ◽  
Vol 19 (3) ◽  
pp. 399-411 ◽  
Author(s):  
N.J. Lane ◽  
R. Dallai ◽  
G.B. Martinucci ◽  
P. Burighel
Keyword(s):  
Thin Section ◽  
Freeze Fracture ◽  
Cell Junctions ◽  
Fracture Study
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