Growth and fine structure of monolayers derived from adult rat adenohypophyseal cell suspensions

In Vitro ◽  
1973 ◽  
Vol 8 (4) ◽  
pp. 301-306 ◽  
Author(s):  
G. Rappay ◽  
Angéla Gyévai ◽  
L. Kondics ◽  
E. Stark
1992 ◽  
Vol 14 (2) ◽  
pp. 130-143 ◽  
Author(s):  
Brigitte Onténiente ◽  
Fatiha Nothias ◽  
Michel Geffard ◽  
Marc Peschanski

1979 ◽  
Vol 57 (19) ◽  
pp. 2006-2020 ◽  
Author(s):  
David G. Davis ◽  
Aurelia Brezeanu

Cell suspensions from root-derived callus tissues of Triticum monococcum L. were examined during growth and senescence. The fine structure of control cultures was compared with the ultrastructural modifications in cultures treated with the compound, methyl-2-(4-(2′,4′-dichlorophenoxy)phenoxy)propanoate (Diclofop-methyl). The control cell suspensions were observed during the cell division phase, the log phase, and the early stationary phase of growth. Senescence is characterized by a loss of ribosomes, alteration of internal membranes of the plastids, vesiculation, formation of granular material in the cytoplasm, rupture of the tonoplast, and an increase in number of lipid bodies. Cells in advanced stages of senescence had few organelles, but rather the cytoplasm consisted almost exclusively of lipid bodies and vesicles. The fine structure of the nuclei and mitochondria was least affected during aging. The ultrastructural effects of herbicide treatment did not completely parallel those of senescing cells, although any distinctions were difficult to sort out. The intensity of herbicide damage depended on both concentration and time (and undoubtedly the physiological state of any particular cell at the time of treatment). Low concentrations (4 μM) at 12 h resulted in plastid damage (analogous to chloroplast damage in photosynthetic tissues treated with herbicides) and in the formation of some myelin figures. At 20 μM concentration for 12 h, very extensive formation of myelin figures was observed in all cells. By 70 h, cells treated with 4 μM herbicide ranged from those with little or no damage to cells with extensive vesicle formation. In contrast, all cells treated with 20 μM herbicide at 70 h were damaged greatly. Some nuclei were present, although the nuclear envelopes were altered. Most organelles were barely recognizable. Extensive vesiculation and lipid formation occurred. These effects were more pronounced in cells treated with 40 μM herbicide at 70 h than in cells treated with lesser concentrations for shorter times. In this case, some organelles were recognizable (plastids and mitochondria) but they were abnormal in that the contents were often granular with only remnants of membranes. Some cells appeared as though the physiological processes were stopped so rapidly that hydrolytic enzymes could not function totally.


1966 ◽  
Vol 71 (2) ◽  
pp. 189-206 ◽  
Author(s):  
Carmen Lemos ◽  
Joseph Pick

1962 ◽  
Vol 12 (2) ◽  
pp. 329-359 ◽  
Author(s):  
Jack Rosenbluth

Nerve cell bodies in the spiral and vestibular ganglia of the adult rat are surrounded by thin (about ten lamellae) myelin sheaths which differ in several respects from typical axonal myelin. In some instances lamellae surrounding perikarya appear as typical major dense lines, and in others as thin Schwann cell sheets in which cytoplasm persists. Discontinuities and irregularities appear in the structure of perikaryal myelin. Lamellae may terminate anywhere within the sheaths; they may bifurcate; they may reverse their direction; or they may merge with each other. The number of lamellae varies from one part of a sheath to another. In addition, the myelin of a single perikaryal sheath may receive contributions from more than one Schwann cell, which overlap and interleave with each other. The ganglion cells are of two types: those which are densely packed with the usual cytoplasmic organelles but have few neurofilaments (granular neurons), and those which exhibit large areas containing few organelles but have a high concentration of neurofilaments (filamented neurons). The latter cell type is ensheathed by myelin which is generally more compact that that surrounding the former. The formation and the physiologic significance of perikaryal myelin are discussed.


1980 ◽  
Vol 36 (2) ◽  
pp. 194-196 ◽  
Author(s):  
G. J. Pilkington ◽  
P. L. Lantos ◽  
J. P. Roscoe

Author(s):  
W. H. Zucker ◽  
R. G. Mason

Platelet adhesion initiates platelet aggregation and is an important component of the hemostatic process. Since the development of a new form of collagen as a topical hemostatic agent is of both basic and clinical interest, an ultrastructural and hematologic study of the interaction of platelets with the microcrystalline collagen preparation was undertaken.In this study, whole blood anticoagulated with EDTA was used in order to inhibit aggregation and permit study of platelet adhesion to collagen as an isolated event. The microcrystalline collagen was prepared from bovine dermal corium; milling was with sharp blades. The preparation consists of partial hydrochloric acid amine collagen salts and retains much of the fibrillar morphology of native collagen.


Author(s):  
E. Horvath ◽  
K. Kovacs ◽  
G. Penz ◽  
C. Ezrin

Follicular structures, in the rat pituitary, composed of cells joined by junctional complexes and possessing few organelles and few, if any, secretory granules, were first described by Farquhar in 1957. Cells of the same description have since been observed in several species including man. The importance of these cells, however, remains obscure. While studying human pituitary glands, we have observed wide variations in the fine structure of follicular cells which may lead to a better understanding of their morphogenesis and significance.


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