Further evidence for entry of infectious bovine rhinotracheitis virus into bovine kidney cells

1978 ◽  
Vol 1 (3) ◽  
pp. 145-150
Author(s):  
Luciano Tiangco Talens ◽  
Yuan Chung Zee
Keyword(s):  
Kidney Cells ◽  
Infectious Bovine Rhinotracheitis ◽  
Bovine Kidney ◽  
Infectious Bovine Rhinotracheitis Virus

STUDIES OF INFECTIOUS BOVINE RHINOTRACHEITIS VIRUS: 1. PLAQUE ASSAY AND SOME CHARACTERISTICS IN BOVINE KIDNEY CELLS

1963 ◽  
Vol 9 (4) ◽  
pp. 567-576 ◽  
Author(s):  
Leslie R. Sabina ◽  
Raymond C. Parker
Keyword(s):  
Infectious Virus ◽  
Plaque Assay ◽  
Growth Curves ◽  
Kidney Cells ◽  
Infectious Bovine Rhinotracheitis ◽  
High Input ◽  
Bovine Kidney ◽  
One Step ◽  
Step Growth ◽  
Infectious Bovine Rhinotracheitis Virus

A reproducible plaquing procedure for infectious bovine rhinotracheitis virus (IBRV) in an established bovine kidney cell line is reported. The validity of this system for quantitative analysis has been established by conventional methods.After infection at different multiplicities, one-step growth curves have shown that the eclipse period for IBRV lasts approximately 4 hours and that the infectious virus increases at a logarithmic rate for 12 to 14 hours. The virus yield with the low and high input is 30 PFU and 210 PFU per cell, respectively. Only 1 to 9% of the total virus is released at 24 hours postinfection. The data presented indicate the half-life of IBRV at 37 °C and 42 °C to be 16 and 3.5 hours, respectively. A comparison of hyperimmune bovine and rabbit sera has shown that 92% of the infective particles are neutralized within 30 minutes.

AN ELECTRON MICROSCOPIC COMPARISON OF BOVINE INFECTIOUS PUSTULAR VULVOVAGINITIS VIRUS AND INFECTIOUS BOVINE RHINOTRACHEITIS VIRUS IN TISSUE CULTURE

1962 ◽  
Vol 8 (3) ◽  
pp. 373-378 ◽  
Author(s):  
I. Grinyer ◽  
M. Savan ◽  
M. J. Studdert
Keyword(s):  
Tissue Culture ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Phase Contrast ◽  
Kidney Cells ◽  
Infectious Bovine Rhinotracheitis ◽  
Electron Microscopic ◽  
Foetal Kidney ◽  
Electron Microscopes ◽  
Infectious Bovine Rhinotracheitis Virus

An electron microscopic study of IPV and IBR viruses isolated from cattle in Canada is presented. A technique is described with which it is possible to observe the cytopathic changes occurring in the same area of a monolayer, of bovine foetal kidney cells, infected with IPV or IBR virus, with both the phase contrast and electron microscopes. The morphology of these viruses was found to be identical and no difference was observed in their intracellular development.

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