Mineralized bone nodules formedin vitro from enzymatically released rat calvaria cell populations

1986 ◽  
Vol 38 (3) ◽  
pp. 143-154 ◽  
Author(s):  
C. G. Bellows ◽  
J. E. Aubin ◽  
J. N. M. Heersche ◽  
M. E. Antosz
Keyword(s):  
Cell Populations ◽  
Rat Calvaria ◽  
Calvaria Cell

A versatile chondrogenic rat calvaria cell line R-tTA-24 that permits tetracycline-regulated gene expression

2000 ◽  
Vol 113 (2) ◽  
pp. 145-150 ◽  
Author(s):  
C. Bergwitz ◽  
T. Wendlandt ◽  
E. Pötter ◽  
I. Glomb ◽  
K. Gras ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Rat Calvaria ◽  
Regulated Gene Expression ◽  
Calvaria Cell

scholarly journals EP2 and EP4 receptors differentially mediate MAPK pathways underlying anabolic actions of prostaglandin E2 on bone formation in rat calvaria cell cultures

Bone ◽  
2009 ◽  
Vol 44 (6) ◽  
pp. 1177-1185 ◽  
Author(s):  
Tomoko Minamizaki ◽  
Yuji Yoshiko ◽  
Katsuyuki Kozai ◽  
Jane E. Aubin ◽  
Norihiko Maeda
Keyword(s):  
Bone Formation ◽  
Prostaglandin E2 ◽  
Cell Cultures ◽  
Mapk Pathways ◽  
Rat Calvaria ◽  
Calvaria Cell

scholarly journals Pleiotropic effects of the steroid hormone 1,25-dihydroxyvitamin D3 on the recruitment of mesenchymal lineage progenitors in fetal rat calvaria cell populations

2006 ◽  
Vol 36 (3) ◽  
pp. 425-433 ◽  
Author(s):  
Shulin Zhang ◽  
Melinda Chan ◽  
Jane E Aubin
Keyword(s):  
Single Cell ◽  
Steroid Hormone ◽  
Cell Populations ◽  
Control Group ◽  
Dihydroxyvitamin D3 ◽  
Mesenchymal Progenitors ◽  
1,25 Dihydroxyvitamin D3 ◽  
Rat Calvaria ◽  
Neural Markers

The steroid hormone 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) inhibits osteogenesis while stimulating adipogenesis in vitro. We hypothesized that 1,25(OH)2D3 redirects the fate of osteoblast/adipocyte bipotential progenitors and other potential progenitors towards adipogenesis, a process possibly underlying the pathogenesis of osteopenic diseases such as osteoporosis. We therefore tested the global effects of 1,25(OH)2D3 on the recruitment of mesenchymal progenitors including osteogenic, chondrogenic, adipogenic and myogenic lineages (colony forming cell (CFC)-osteoblast (CFC-O), CFC-chondrocyte (CFC-C), CFC-adipocyte (CFC-A), and CFC-myoblast (CFC-M) respectively) in rat calvaria (RC) cell populations using gene expression profiling of single cell-derived colonies. Based on expression of lineage specific transcripts, 86% of single cell-derived colonies in untreated cultures simultaneously co-expressed transcripts of two, three, or four of the mesenchymal lineages tested. The distribution of mesenchymal progenitors in 1,25(OH)2D3-treated cultures was significantly changed compared with the control group, i.e. CFC-O were reduced (from 6 to 0%) and CFC-O/A bipotential (0 to 8.2%), CFC-C (4 to 10.2%) and CFC-Fibroblast (CFC-F) (4 to 16%) were increased. 1,25(OH)2D3 did not affect the frequency of tri- or tetra-lineage colonies. Single lineage CFC-A colonies were not detected in either the control or 1,25(OH)2D3 treatment group under the conditions tested.Since the parietal bones used for cell isolation derive from neuroectoderm, we also analyzed for expression of the neural markers nestin and β3 tubulin in these colonies. Surprisingly, 90% (45 of 50) of the colonies in the control group expressed neural markers, a frequency not changed by 1,25(OH)2D3 treatment. The current studies demonstrate the global and developmental stage-specific effects of 1,25(OH)2D3 on mesenchymal lineage progenitors, and suggest that the effects of 1,25(OH)2D3 on osteogenesis and adipogenesis in RC populations are mediated, at least in part, by increased recruitment of CFC-O/A, but not CFC-A type precursors.

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