Aluminum Accelerates Osteoblastic Differentiation But is Cytotoxic in Long-Term Rat Calvaria Cell Cultures

1999 ◽  
Vol 65 (1) ◽  
pp. 59-65 ◽  
Author(s):  
C. G. Bellows ◽  
J. N. M. Heersche ◽  
J. E. Aubin
Keyword(s):  
Cell Cultures ◽  
Osteoblastic Differentiation ◽  
Rat Calvaria ◽  
Calvaria Cell

scholarly journals EP2 and EP4 receptors differentially mediate MAPK pathways underlying anabolic actions of prostaglandin E2 on bone formation in rat calvaria cell cultures

Bone ◽  
2009 ◽  
Vol 44 (6) ◽  
pp. 1177-1185 ◽  
Author(s):  
Tomoko Minamizaki ◽  
Yuji Yoshiko ◽  
Katsuyuki Kozai ◽  
Jane E. Aubin ◽  
Norihiko Maeda
Keyword(s):  
Bone Formation ◽  
Prostaglandin E2 ◽  
Cell Cultures ◽  
Mapk Pathways ◽  
Rat Calvaria ◽  
Calvaria Cell

scholarly journals LIF, but Not IL-6, Regulates Osteoprogenitor Differentiation in Rat Calvaria Cell Cultures: Modulation by Dexamethasone

1998 ◽  
Vol 13 (2) ◽  
pp. 175-184 ◽  
Author(s):  
Luc Malaval ◽  
Ashwani K. Gupta ◽  
Fina Liu ◽  
Pierre D. Delmas ◽  
Jane E. Aubin
Keyword(s):  
Cell Cultures ◽  
Rat Calvaria ◽  
Calvaria Cell

scholarly journals The EP4-ERK-dependent pathway stimulates osteo-adipogenic progenitor proliferation resulting in increased adipogenesis in fetal rat calvaria cell cultures

2012 ◽  
Vol 97 (3-4) ◽  
pp. 97-102 ◽  
Author(s):  
Tomoko Minamizaki ◽  
Yuji Yoshiko ◽  
Hirotaka Yoshioka ◽  
Katsuyuki Kozai ◽  
Jane E. Aubin ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Rat Calvaria ◽  
Fetal Rat ◽  
Dependent Pathway ◽  
Calvaria Cell

scholarly journals Stimulation by defined parathyroid hormone fragments of cell proliferation in skeletal-derived cell cultures

1990 ◽  
Vol 272 (3) ◽  
pp. 781-785 ◽  
Author(s):  
D Sömjen ◽  
I Binderman ◽  
K D Schlüter ◽  
E Wingender ◽  
H Mayer ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Cyclic Amp ◽  
Dna Synthesis ◽  
Cell Cultures ◽  
Thymidine Incorporation ◽  
Bone Cells ◽  
Functional Domain ◽  
Independent Manner ◽  
Rat Calvaria ◽  
Calvaria Cell

We have reported previously that parathyroid hormone (PTH) acts on cultured bone cells to stimulate creatine kinase (CK) activity and [3H]thymidine incorporation into DNA via phosphoinositide turnover, in addition to its other actions via increased cyclic AMP production. We also found that mid-region fragments of PTH stimulate [3H]thymidine incorporation into avian chondrocytes. In the present study of mammalian systems, we demonstrate differential effects of defined synthetic PTH fragments on CK activity and DNA synthesis, as compared with cyclic AMP production, in osteoblast-enriched embryonic rat calvaria cell cultures, in an osteoblast-like clone of rat osteosarcoma cells (ROS 17/2.8) and in chondroblasts from rat epiphysial cartilage cell cultures. Unlike full-length bovine (b)PTH-(1-84) or the fully effective shorter fragment human (h)PTH-(1-34), fragments lacking the N-terminal region of the hormone did not increase cyclic AMP formation, whereas they did stimulate increases in both DNA synthesis and CK activity. Moreover, the PTH fragment hPTH-(28-48) at 10 microM inhibited the increase in cyclic AMP caused by 10 nM-bPTH-(1-84). The increase of CK activity in ROS 17/2.8 cells caused by bPTH-(1-84) or hPTH-(28-48) was completely inhibited by either cycloheximide or actinomycin D, as was shown previously for rat calvaria cell cultures. These results indicated the presence of a functional domain of PTH in the central part of the molecule which exerts its mitogenic-related effects on osteoblast- and chondroblast-like cells in a cyclic AMP-independent manner. Since cyclic AMP formation by PTH leads to bone resorption, specific mid-region fragments of PTH might prove suitable for use in vivo to induce bone formation without concomitant resorption.

scholarly journals Stanniocalcin 1 Stimulates Osteoblast Differentiation in Rat Calvaria Cell Cultures

Endocrinology ◽  
2003 ◽  
Vol 144 (9) ◽  
pp. 4134-4143 ◽  
Author(s):  
Yuji Yoshiko ◽  
Norihiko Maeda ◽  
Jane E. Aubin
Keyword(s):  
Cell Cultures ◽  
Osteoblast Differentiation ◽  
Rat Calvaria ◽  
Calvaria Cell

Functional neutrophils from long-term murine bone marrow cell cultures

1987 ◽  
Vol 100 (1-2) ◽  
pp. 223-233 ◽  
Author(s):  
P.H. Hart ◽  
L.K. Spencer ◽  
P.A. Kenny ◽  
A.F. Lopez ◽  
P.J. McDonald ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cell ◽  
Cell Cultures ◽  
Marrow Cell ◽  
Murine Bone Marrow ◽  
Murine Bone Marrow Cell
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