Visualization of primate high density lipoproteins isolated by density gradient ultracentrifugation

Lipids ◽  
1978 ◽  
Vol 13 (1) ◽  
pp. 88-91 ◽  
Author(s):  
Jerome L. Hojnacki ◽  
Robert J. Nicolosi ◽  
Norma Llansa ◽  
K. C. Hayes
Keyword(s):  
Density Gradient ◽  
High Density ◽  
High Density Lipoproteins ◽  
Density Gradient Ultracentrifugation

Incorporation of radioactive phospholipid into subclasses of high-density lipoproteins

1983 ◽  
Vol 244 (5) ◽  
pp. E513-E516 ◽  
Author(s):  
A. R. Tall ◽  
C. B. Blum ◽  
S. M. Grundy
Keyword(s):  
Density Gradient ◽  
Specific Activity ◽  
High Density ◽  
High Density Lipoproteins ◽  
Equilibrium Density ◽  
Density Gradient Ultracentrifugation ◽  
Hdl Subfractions ◽  
Time Points ◽  
Plasma High Density

The incorporation of orally administered phospholipid into plasma high-density lipoproteins (HDL) was studied in three subjects. Plasma was analyzed by equilibrium density gradient ultracentrifugation, 5, 6, and 8 h after ingestion of 1.1 g [3H-choline, 14C-dilinoleoyl]phosphatidylcholine. At all time points in all subjects, there was a peak of phosphatidylcholine specific activity in fractions of density approximately 1.10-1.13 g/ml, corresponding to the subclass previously designated HDL2a. There was also a more variable, smaller peak of specific activity of phospholipids in HDL2b (1.063-1.100 g/ml) and in fractions of density approximately 1.19 g/ml. In the 1.10-1.13 fraction, 97 and 71%, respectively, of the 3H and 14C radioactivity were in phospholipids. The 3H/14C ratio was similar in phospholipids of HDL subfractions, the d less than 1.07 fraction, and in the administered phospholipid. The results show preferential transfer or exchange or absorbed phosphatidylcholine into specific subclasses of HDL.

Precipitation with polyethylene glycol and density-gradient ultracentrifugation compared for determining high-density lipoprotein subclasses HDL2 and HDL3

1991 ◽  
Vol 37 (2) ◽  
pp. 238-240 ◽  
Author(s):  
Kurt Widhalm ◽  
Renate Pakosta
Keyword(s):  
Polyethylene Glycol ◽  
High Density Lipoprotein ◽  
Density Gradient ◽  
Density Lipoprotein ◽  
Correlation Coefficients ◽  
High Density ◽  
Precipitation Method ◽  
Density Gradient Ultracentrifugation ◽  
Serum Samples ◽  
Lipoprotein Subfractions

Abstract The purpose of this study was to compare quantification of cholesterol in high-density lipoprotein subfractions HDL2 and HDL3 by precipitation with polyethylene glycol (PEG) with that by density-gradient ultracentrifugation. Fresh serum samples from 32 fasting, obese children were analyzed with precipitation reagent "Quantolip" (Immuno AG), and then fractionated with a Beckman TL 100 ultracentrifuge with a swinging-bucket rotor. After centrifugation we carefully removed the supernate with a syringe and measured the cholesterol from each fraction enzymatically with CHOD-PAP reagent (Boehringer Mannheim). The low-density lipoprotein (LDL-), HDL2-, and HDL3-cholesterol values measured by ultracentrifugation did not differ significantly from those obtained by precipitation; the correlation coefficients (r) between the two methods were 0.96 for LDL, 0.75 for HDL2, and 0.96 for HDL3. The relatively simple PEG precipitation method used in this study measures total HDL and its major subclasses HDL2 and HDL3 with accuracy and precision comparable with those of the well-established ultracentrifugation method.

scholarly journals Layer-by-Layer Sorting of Rhenium Disulfide via High-Density Isopycnic Density Gradient Ultracentrifugation

Nano Letters ◽  
2016 ◽  
Vol 16 (11) ◽  
pp. 7216-7223 ◽  
Author(s):  
Joohoon Kang ◽  
Vinod K. Sangwan ◽  
Joshua D. Wood ◽  
Xiaolong Liu ◽  
Itamar Balla ◽  
...  
Keyword(s):  
Density Gradient ◽  
High Density ◽  
Density Gradient Ultracentrifugation ◽  
Layer By Layer
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