Large-scale preparation of human low- and high-density lipoproteins by density gradient centrifugation using iodixanol

2007 ◽  
Vol 367 (1) ◽  
pp. 137-139 ◽  
Author(s):  
David Billington ◽  
Emma Maxwell ◽  
John M. Graham ◽  
Paul Newland
Keyword(s):  
Density Gradient ◽  
Large Scale ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
High Density ◽  
High Density Lipoproteins ◽  
Large Scale Preparation ◽  
Scale Preparation

The large-scale preparation of purified sporozoites of Eimeria spp. by metrizamide density-gradient centrifugation

Parasitology ◽  
1984 ◽  
Vol 88 (3) ◽  
pp. 515-519 ◽  
Author(s):  
M. H. Wisher ◽  
M. E. Rose
Keyword(s):  
Density Gradient ◽  
Large Scale ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Density Gradients ◽  
Large Scale Preparation ◽  
Eimeria Spp ◽  
Scale Preparation ◽  
The Mean

SUMMARYA method is described for the reproducible purification of greater than 1·0 × 108 sporozoites of Eimeria spp. by centrifugation on metrizamide density-gradients. The mean contamination of the purified sporozoite fraction by sporocysts and sporocyst debris was less than 6% and the recovered sporozoites were fully viable, infective and ultrastructurally intact.

scholarly journals Preparation and characterization of muscarinic-acetylcholine-receptor-enriched membranes from pig atria

1982 ◽  
Vol 202 (2) ◽  
pp. 475-481 ◽  
Author(s):  
G L Peterson ◽  
M I Schimerlik
Keyword(s):  
Acetylcholine Receptor ◽  
Large Scale ◽  
Specific Activity ◽  
Gradient Centrifugation ◽  
Muscarinic Acetylcholine Receptor ◽  
Marker Enzyme ◽  
Muscarinic Acetylcholine ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Quinuclidinyl Benzilate

A procedure was developed for the large scale preparation of membranes from pig atria which are enriched 10-13 fold in the muscarinic acetylcholine receptor. The procedure involved differential centrifugation and sucrose-gradient centrifugation in solutions containing 150 mM-NaClO4 and 5 mM-EDTA to minimize membrane aggregation. The final membrane preparation bound about 1.1 pmol of L-quinuclidinyl benzilate/mg of protein. Comparable results were obtained with either fresh or frozen tissue. About the same yield (120 pmol of L-quinuclidinyl benzilate sites/100 g of tissue) and specific activity of membranes were obtained from different regions of the atria. The final preparation was stable at -80 degrees C in buffered sucrose solutions. The membranes appeared mostly as sheets or fragments and partly as closed vesicles in the electron microscope and were heterogeneous in isopycnic Percoll gradients. Marker enzyme studies showed that the receptor was enriched in parallel with the plasma membrane markers guanylate cyclase (particulate form) and (Na+ + K+)-activated ATPase. Some contamination by mitochondrial outer and endoplasmic reticulum membranes was evident from the distribution of monoamine oxidase and glucose-6-phosphatase activity, but the preparation was largely free of sarcoplasmic reticulum, mitochondrial inner, and lysosomal membranes.

Sodium metatungstate: a new heavy-mineral separation medium for the extraction of conodonts from insoluble residues

1988 ◽  
Vol 62 (2) ◽  
pp. 314-316 ◽  
Author(s):  
Stanley T. Krukowski
Keyword(s):  
Density Gradient ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
High Density ◽  
Mineral Separation ◽  
Neutral Ph ◽  
Low Viscosity ◽  
Inorganic Mineral ◽  
Sodium Polytungstate ◽  
Separation Medium

Sodium metatungstate (SMT), 3Na2WO4 · 9WO3 · H2O, is a nontoxic (Sax, 1979), high-density (1.00-3.10 g/cm3) separating compound which results in nearly neutral (pH 6) solutions of relatively low viscosity. It was presented as a medium for density gradient centrifugation by Plewinsky and Kamp (1984). Since then, investigators have used SMT (or sodium polytungstate) as a high-density medium for routine mineral separations, for recovery of conodonts from insoluble residues, for separation of various feldspar species from one another, and for segregation of inorganic mineral fractions (ash) from coal.

Uranyl Acetate and Rotary Shadowing to Increase the Contrast of Small Aggregated Virus Particles

1969 ◽  
Vol 27 ◽  
pp. 424-425
Author(s):  
Lee F. Ellis ◽  
Richard M. Van Frank ◽  
Walter J. Kleinschmidt
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Density Gradient ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Uranyl Acetate ◽  
Interferon Inducer ◽  
Virus Particles ◽  
Staining Methods ◽  
Rotary Shadowing

The extract from Penicillum stoliniferum, known as statolon, has been purified by density gradient centrifugation. These centrifuge fractions contained virus particles that are an interferon inducer in mice or in tissue culture. Highly purified preparations of these particles are difficult to enumerate by electron microscopy because of aggregation. Therefore a study of staining methods was undertaken.

Purification of the Antihemophilic Factor by Gel Filtration on Agarose

1969 ◽  
Vol 22 (03) ◽  
pp. 577-583 ◽  
Author(s):  
M.M.P Paulssen ◽  
A.C.M.G.B Wouterlood ◽  
H.L.M.A Scheffers
Keyword(s):  
Factor Viii ◽  
Plasma Proteins ◽  
Large Scale ◽  
Gel Filtration ◽  
Large Scale Preparation ◽  
Scale Preparation ◽  
Antihemophilic Factor

SummaryFactor VIII can be isolated from plasma proteins, including fibrinogen by chromatography on agarose. The best results were obtained with Sepharose 6B. Large scale preparation is also possible when cryoprecipitate is separated by chromatography. In most fractions containing factor VIII a turbidity is observed which may be due to the presence of chylomicrons.The purified factor VIII was active in vivo as well as in vitro.

The Combining Ability of Glycoproteins IIb, IIIa and Ca2+ in EDTA-Treated Nonaggregable Platelets

1983 ◽  
Vol 50 (04) ◽  
pp. 848-851 ◽  
Author(s):  
Marjorie B Zucker ◽  
David Varon ◽  
Nicholas C Masiello ◽  
Simon Karpatkin
Keyword(s):  
Density Gradient ◽  
Combining Ability ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Electrophoretic Pattern ◽  
Sucrose Density Gradient ◽  
Irreversible Loss ◽  
Sucrose Density Gradient Centrifugation ◽  
Crossed Immunoelectrophoresis ◽  
Triton X

SummaryPlatelets deprived of calcium and incubated at 37° C for 10 min lose their ability to bind fibrinogen or aggregate with ADP when adequate concentrations of calcium are restored. Since the calcium complex of glycoproteins (GP) IIb and IIIa is the presumed receptor for fibrinogen, it seemed appropriate to examine the behavior of these glycoproteins in incubated non-aggregable platelets. No differences were noted in the electrophoretic pattern of nonaggregable EDTA-treated and aggregable control CaEDTA-treated platelets when SDS gels of Triton X- 114 fractions were stained with silver. GP IIb and IIIa were extracted from either nonaggregable EDTA-treated platelets or aggregable control platelets with calcium-Tris-Triton buffer and subjected to sucrose density gradient centrifugation or crossed immunoelectrophoresis. With both types of platelets, these glycoproteins formed a complex in the presence of calcium. If the glycoproteins were extracted with EDTA-Tris-Triton buffer, or if Triton-solubilized platelet membranes were incubated with EGTA at 37° C for 30 min, GP IIb and IIIa were unable to form a complex in the presence of calcium. We conclude that inability of extracted GP IIb and IIIa to combine in the presence of calcium is not responsible for the irreversible loss of aggregability that occurs when whole platelets are incubated with EDTA at 37° C.

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