Comparative analysis of population characteristics of the brown planthopper,Nilaparvata lugens stål, between wet and dry rice cropping seasons in West Java, Indonesia

1993 ◽  
Vol 35 (1) ◽  
pp. 113-137 ◽  
Author(s):  
Hiroichi Sawada ◽  
Ayi Kusmayadi ◽  
S. W. Gaib Subroto ◽  
Edi Suwardiwijaya ◽  
Mustaghfirin
2016 ◽  
Vol 35 (2) ◽  
pp. 99 ◽  
Author(s):  
Baehaki S. E. ◽  
E. H. Iswanto ◽  
D. Munawar

Research on brown planthopper (BPH), (Nilaparvata lugens (Stal.) resistant to insecticide using dipping method was carried out in the wet season (WS) of 2011/2012 at Indonesian Center for Rice Research, Sukamandi. BPH field population from Sukamandi, West Java, known as Sukamandi BPH population and Juwiring, Central Java, as Juwiring BPH population, were measured their degree of resistance to insecticides to be compared with the BPH biotype 1 of screen house population. The insecticides used were imidacloprid, ethiprole, thiamethoxam, fipronil, BPMC, MIPC, buprofezin, cypermethrin and cyhalothrin. The resistance ratios (RR) were measured by LC50 of BPH from fields/LC50 of BPH from screen house. Results showed that Sukamandi BPH population was moderately resistance to imidacloprid and cypermethrin, but was low resistance to buprofezin, and was decreasing into susceptibility to fipronil, thiamethoxam and cyhalothrin, where as to ethiprole, BPMC dan MIPC the Sukamandi BPH was still susceptible. The Juwiring BPH population was low resistance to imidacloprid, buprofezin, cypermethrin and cyhalothrin, but the Juwiring BPH was decreasing into susceptibility to BPMC, ethiprole and fipronil. The population was still susceptible to thiamethoxam and MIPC.


2018 ◽  
Vol 11 (2) ◽  
pp. 122-132
Author(s):  
Yadi Suryadi ◽  
Wartono Wartono ◽  
Dwi Ningsih Susilowati ◽  
Puji Lestari ◽  
Cyntia Nirmalasari ◽  
...  

AbstrakBeauveria spp. merupakan salah satu jamur entomopatogen yang dapat digunakan sebagai agen biokontrol terhadap berbagai serangga hama. Tujuan penelitian ini adalah untuk menguji patogenitas Beauveria spp. terhadap serangga wereng coklat (Nilaparvata lugens Stål). Isolat-isolat Beauveria spp. diisolasi dari serangga walang sangit dari Situ Gede, Bogor, Jawa Barat. Identifikasi jamur dilakukan berdasarkan analisis data sekuen dari daerah Internal Transcribed Spacer (ITS) rDNA. Hasil uji menunjukkan bahwa patogenisitas isolat Stgd 5(14)2 dan Stgd 7(14)2 menghasilkan tingkat mortalitas 100% terhadap N. lugens. Isolat Stgd 5(14)2 dan Stgd 7(14)2 memiliki nilai LT50 yang rendah, danmenyebabkan kematian yang cepat terhadap wereng coklat. Hasil analisis filogenetik menunjukkan bahwa sekuen Stgd 5(14)2 dan Stgd 7(14)2 termasuk ke dalam spesies Beauveria bassiana s.str. Hasil studi ini merupakan kajian awal terhadap patogenisitas jamur entomopatogen B. bassiana terhadap wereng coklat, dan distribusinya di pertanaman padi Situ Gede, Jawa Barat.Abstract Beauveria spp. is one of the entomopathogenic fungi that can be used as biocontrol agents against various insect pests, including brown planthopper (Nilaparvata lugens Stal). This study aimed to test the pathogenicity of Beauveria spp. against N. lugens. Beauveria spp. were isolated from the rice stink bug insects, collected from Situ Gede, Bogor, West Java. Fungal identification was carried out based on the internal transcribed spacer (ITS) rDNA analysis. The pathogenicity assay revealed that Stgd 5(14)1 and Stgd 7(14)2 isolates were virulent against N. lugens, with a mortality of 100%. The LT50 (median lethal time) determination, indicated that Stgd 5(14)2  and Stgd 7(14)2 isolates had low value indicating a faster mortality in brown planthopper insects. Phylogenetic analysis showed that the sequences of Stgd 5(14)2 and Stgd 7(14)2 belong to Beauveria bassiana s.str. This is the preliminary pathogenicity trial of entomopathogenic fungi B. bassiana against brown planthopper and their distribution in rice-growing, Situ Gede area in West Java.


PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47413 ◽  
Author(s):  
Jiranan Piyaphongkul ◽  
Jeremy Pritchard ◽  
Jeff Bale

2015 ◽  
Vol 105 (4) ◽  
pp. 390-398 ◽  
Author(s):  
P.-J. Wan ◽  
L. Yang ◽  
S.-Y. Yuan ◽  
Y.-H. Tang ◽  
Q. Fu ◽  
...  

AbstractThe brown planthopper Nilaparvata lugens is a serious phloem-feeding pest of rice in China. The current study focuses on a saccharopine dehydrogenase (SDH) that catalyzes the penultimate reaction in biosynthesis of the amino acid lysine (Lys), which plays a role in insect growth and carnitine production (as a substrate). The protein, provisionally designated as NlylsSDH [a SDH derived from yeast-like symbiont (YLS) in N. lugens], had a higher transcript level in abdomens, compared with heads, wings, legs and thoraces, which agrees with YLS distribution in N. lugens. Ingestion of Nlylssdh targeted double-stranded RNA (dsNlylssdh) for 5, 10 and 15 days decreased the mRNA abundance in the hoppers by 47, 70 and 31%, respectively, comparing with those ingesting normal or dsegfp diets. Nlylssdh knockdown slightly decreased the body weights, significantly delayed the development of females, and killed approximately 30% of the nymphs. Moreover, some surviving adults showed two apparent phenotypic defects: wing deformation and nymphal cuticles remained on tips of the legs and abdomens. The brachypterours/macropterours and sex ratios (female/male) of the adults on the dsRNA diet were lowered compared with the adults on diets without dsRNA. These results suggest that Nlylssdh encodes a functional SDH protein. The adverse effect of Nlylssdh knockdown on N. lugens implies the importance of Lys in hopper development. This study provides a proof of concept example that Nlylssdh could serve as a possible dsRNA-based pesticide for planthopper control.


Agrologia ◽  
2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Martua Suhunan Sianipar

Brown Planthopper (Nilaparvata lugens Stal.) is a main pest of paddy. The purpose of this research is to study the population of Brown Planthopper and the natural enemy. The method of this research using survey. Survey was conducted in three experimental plots at JatisariSubdistrict, Karawang District, West Javaand in the greenhouse of the Department of Plant Pests and Deseases, Faculty of Agriculture,  Universitas Padjadjaran. The sample were collected by systematically diagonal using yellow stiky trap and manually or visual counting. The result showed population fluctuation BPH below the economy line where is temperature, humidity and rainfall did not significantly effect to the BPH population. It was indicated by the regression analysis of each temperature Y= (- 24.225 + 1.008 X1 ; R2 =0,020; P/Sign = 0.660),  Humidity  (Y=- 32.337 + 0.424 X2; R2  = 0,124; P/ Sign  = 0.261) and Rainfall (Y=1.707 + 0.090 X3 ; R2  = 0.050; P/ Sign = 0.487). There is a relation between temperature, humidity and rainfall to BPH (Y = - 123 + 2.685 T + 0.649 RH - 0.14 CH; R2 = 0.236; P/ Sign = 0.516) dit not significantly because as the value of significance (P = Sign)  > 0.05.8. The diversity index was moderate 1.88


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