A high-density culture of hepatocytes using porous substrate for use as a bioartificial liver

1999 ◽  
Vol 2 (2) ◽  
pp. 124-128 ◽  
Author(s):  
Kennichi Yanagi ◽  
Hirotoshi Miyoshi ◽  
Norio Ohshima
Keyword(s):  
Bioartificial Liver ◽  
High Density ◽  
Porous Substrate ◽  
High Density Culture ◽  
Culture Of Hepatocytes

Large‐scale high‐density culture of hepatocytes in a liver microsystem with mimicked sinusoid blood flow

2018 ◽  
Vol 12 (12) ◽  
pp. 2266-2276
Author(s):  
Jing Liu ◽  
Chengpan Li ◽  
Shaohui Cheng ◽  
Shengnan Ya ◽  
Dayong Gao ◽  
...  
Keyword(s):  
Blood Flow ◽  
Large Scale ◽  
High Density ◽  
High Density Culture ◽  
Culture Of Hepatocytes

A packed-bed reactor utilizing porous resin enables high density culture of hepatocytes

1992 ◽  
Vol 37 (3) ◽  
pp. 316-320 ◽  
Author(s):  
Kennichi Yanagi ◽  
Hirotoshi Miyoshi ◽  
Hideki Fukuda ◽  
Norio Ohshima
Keyword(s):  
Packed Bed ◽  
High Density ◽  
Packed Bed Reactor ◽  
High Density Culture ◽  
Porous Resin ◽  
Culture Of Hepatocytes

scholarly journals YAP Promotes Cell Proliferation and Stemness Maintenance of Porcine Muscle Stem Cells under High-Density Condition

Cells ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 3069
Author(s):  
Zheng Liu ◽  
Ling Lin ◽  
Haozhe Zhu ◽  
Zhongyuan Wu ◽  
Xi Ding ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Large Scale ◽  
High Density ◽  
Meat Production ◽  
Density Condition ◽  
High Density Culture ◽  
Differentiation Potential ◽  
Muscle Stem Cells ◽  
Cultured Meat

Muscle stem cells (MuSCs) isolated ex vivo are essential original cells to produce cultured meat. Currently, one of the main obstacles for cultured meat production derives from the limited capacity of large-scale amplification of MuSCs, especially under high-density culture condition. Here, we show that at higher cell densities, proliferation and differentiation capacities of porcine MuSCs are impaired. We investigate the roles of Hippo-YAP signaling, which is important regulators in response to cell contact inhibition. Interestingly, abundant but not functional YAP proteins are accumulated in MuSCs seeded at high density. When treated with lysophosphatidic acid (LPA), the activator of YAP, porcine MuSCs exhibit increased proliferation and elevated differentiation potential compared with control cells. Moreover, constitutively active YAP with deactivated phosphorylation sites, but not intact YAP, promotes cell proliferation and stemness maintenance of MuSCs. Together, we reveal a potential molecular target that enables massive MuSCs expansion for large-scale cultured meat production under high-density condition.

scholarly journals Hyper-intensive farming white shrimp Litopenaeus vannamei (Decapoda: Penaeidae) in a seawater tank under semi-controlled conditions

2013 ◽  
Vol 5 (1) ◽  
pp. 69-74
Author(s):  
A. A. Ortega-Salas ◽  
L. A. Rendón M.
Keyword(s):  
Litopenaeus Vannamei ◽  
Maximum Likelihood Method ◽  
High Density ◽  
White Shrimp ◽  
Likelihood Method ◽  
Food Conversion ◽  
High Density Culture ◽  
Intensive Farming ◽  
Controlled Conditions ◽  
Commercial Size

Shrimp development to a commercial size in high density culture saves food and avoids predators and disease. Our study was conducted to calculate the growth of white shrimp Litopenaeus vannamei by hyper-intensive cultivation under semi-controlled conditions. We seeded at a density of 550 shrimp per m3 during the first cycle and 400 shrimp per m3 in the second cycle in an outdoor tank of 6m3or 6m2 covered with mesh, constant aeration. The shrimp were fed Artemia franciscana during the first two weeks and camaronina pellets (35% protein) as required, in food baskets, aftterwards. The temperature ranged from 22,3 to 31,3°C, pH 7,5-8,7, oxygen 4,26±1,43. The tanks are siphoned of debris every other day, and water was replaced according to a program. The food conversion ratio (FCR) was 1:1,3. The shrimp were measured weekly to calculate growth with the Bertalanffy model. Survival in the first cycle was 95,8 ( 97,9% for the second cycle). Population parameters (maximum likelihood method) for the first cycle were k=0,0301, L∞ =322,16 and t0 =-0,8852; second cycle: k=0,0203, L∞ =294,42 and t0 =-5,3771. There was rapid growth during the first 10 weeks. Biomass was 27kg for the first cycle (second: 16kg). KEY WORDSGrowth, high density, survival, biomass, semi-controlled conditions. 

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