Whole-genome analysis of human astrocytic tumors by comparative genomic hybridization

2000 ◽  
Vol 17 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Motohiko Maruno ◽  
Hirotomo Ninomiya ◽  
A. K. M. Ghulam Muhammad ◽  
Masayuki Hirata ◽  
Amami Kato ◽  
...  
Keyword(s):  
Comparative Genomic Hybridization ◽  
Genome Analysis ◽  
Comparative Genomic ◽  
Whole Genome ◽  
Astrocytic Tumors ◽  
Genomic Hybridization ◽  
Whole Genome Analysis

scholarly journals Comparison of Whole Genome Amplification Methods for Analysis of DNA Extracted from Microdissected Early Breast Lesions in Formalin-Fixed Paraffin-Embedded Tissue

ISRN Oncology ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Nona Arneson ◽  
Juan Moreno ◽  
Vladimir Iakovlev ◽  
Arezou Ghazani ◽  
Keisha Warren ◽  
...  
Keyword(s):  
Comparative Genomic Hybridization ◽  
Whole Genome Amplification ◽  
Comparative Genomic ◽  
Whole Genome ◽  
Genomic Hybridization ◽  
Genome Amplification ◽  
Formalin Fixed Paraffin ◽  
Ffpe Samples ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

To understand cancer progression, it is desirable to study the earliest stages of its development, which are often microscopic lesions. Array comparative genomic hybridization (aCGH) is a valuable high-throughput molecular approach for discovering DNA copy number changes; however, it requires a relatively large amount of DNA, which is difficult to obtain from microdissected lesions. Whole genome amplification (WGA) methods were developed to increase DNA quantity; however their reproducibility, fidelity, and suitability for formalin-fixed paraffin-embedded (FFPE) samples are questioned. Using aCGH analysis, we compared two widely used approaches for WGA: single cell comparative genomic hybridization protocol (SCOMP) and degenerate oligonucleotide primed PCR (DOP-PCR). Cancer cell line and microdissected FFPE breast cancer DNA samples were amplified by the two WGA methods and subjected to aCGH. The genomic profiles of amplified DNA were compared with those of non-amplified controls by four analytic methods and validated by quantitative PCR (Q-PCR). We found that SCOMP-amplified samples had close similarity to non-amplified controls with concordance rates close to those of reference tests, while DOP-amplified samples had a statistically significant amount of changes. SCOMP is able to amplify small amounts of DNA extracted from FFPE samples and provides quality of aCGH data similar to non-amplified samples.

Clinical evidence of distinct subgroups of astrocytic tumors defined by comparative genomic hybridization

2000 ◽  
Vol 31 (5) ◽  
pp. 608-614 ◽  
Author(s):  
Takafumi Nishizaki ◽  
Hisashi Kubota ◽  
Kei Harada ◽  
Katsumi Harada ◽  
Haruhide Ito ◽  
...  
Keyword(s):  
Comparative Genomic Hybridization ◽  
Clinical Evidence ◽  
Comparative Genomic ◽  
Astrocytic Tumors ◽  
Genomic Hybridization

Recurrent gain of chromosome arm 7q in low-grade astrocytic tumors studied by comparative genomic hybridization

1996 ◽  
Vol 15 (4) ◽  
pp. 199-205 ◽  
Author(s):  
Evelin Schröck ◽  
Collin Blume ◽  
Marie-Christine Meffert ◽  
Stanislas du Manoir ◽  
Wolf Bersch ◽  
...  
Keyword(s):  
Comparative Genomic Hybridization ◽  
Comparative Genomic ◽  
Low Grade ◽  
Astrocytic Tumors ◽  
Genomic Hybridization ◽  
Chromosome Arm

Chromosomal losses and gains in meningiomas: Comparative genomic hybridization (CGH) study of the whole genome

1998 ◽  
Vol 20 (7) ◽  
pp. 612-616 ◽  
Author(s):  
Motohiko Maruno ◽  
Toshiki Yoshimine ◽  
Ghulam Muhammad ◽  
Hirotomo Ninomiya ◽  
Toru Hayakawa
Keyword(s):  
Comparative Genomic Hybridization ◽  
Comparative Genomic ◽  
Whole Genome ◽  
Genomic Hybridization
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