DNA ploidy level and S-phase fraction as prognostic factors in breast cancer

1990 ◽  
Vol 20 (5) ◽  
pp. 491-497 ◽  
Author(s):  
Takao Yokoe ◽  
Masaru Izuo ◽  
Isunehiro Ishida ◽  
Yuichi Iino ◽  
Tadakazu Kawai
Keyword(s):  
Breast Cancer ◽  
Prognostic Factors ◽  
Ploidy Level ◽  
Dna Ploidy ◽  
S Phase ◽  
Phase Fraction

scholarly journals Evaluation of Prognostic Factors Following Flow-Cytometric DNA Analysis after Cytokeratin Labelling: I. Breast Cancer

2002 ◽  
Vol 24 (4-5) ◽  
pp. 135-145 ◽  
Author(s):  
Pauline Wimberger ◽  
Peter Hillemanns ◽  
Thomas Kapsner ◽  
Hermann Hepp ◽  
Rainer Kimmig
Keyword(s):  
Breast Cancer ◽  
Prognostic Factors ◽  
Tumor Cells ◽  
Dna Ploidy ◽  
Gynecologic Oncology ◽  
Univariate Analysis ◽  
Prognostic Significance ◽  
S Phase ◽  
Phase Fraction ◽  
Flow Cytometric

In gynecologic oncology valid prognostic factors are necessary to estimate the course of disease and to define biologically similar subgroups for analysis of therapeutic efficacy. The presented study is a prospective study concerning prognostic significance of DNA ploidy and S‐phase fraction in breast cancer following enrichment of tumor cells by cytokeratin labelling. Epithelial cells were labeled by FITC‐conjugated cytokeratin antibody (CK 5, 6, 8, and CK 17) prior to flow cytometric cell cycle analysis in 327 fresh specimens of primary breast cancer. Univariate analysis in breast cancer detected the prognostic significance of DNA‐ploidy, S‐phase fraction and CV (coefficient of variation) of G0G1‐peak of tumor cells for clinical outcome, especially for nodal‐negative patients. Multivariate analysis could not confirm prognostic evidence of DNA‐ploidy and S‐phase fraction. In conclusion, in breast cancer no clinical significance for determination of DNA‐parameters was found.

scholarly journals Lack of prognostic significance of DNA ploidy and S phase fraction in breast cancer

1992 ◽  
Vol 66 (5) ◽  
pp. 925-929 ◽  
Author(s):  
PD Stanton ◽  
TG Cooke ◽  
SJ Oakes ◽  
J Winstanley ◽  
S Holt ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Dna Ploidy ◽  
Prognostic Significance ◽  
S Phase ◽  
Phase Fraction

Linell Classification of Breast Cancer Morphology Compared to Histologic Grading, S-Phase Fraction and DNA-Ploidy

1997 ◽  
Vol 193 (7) ◽  
pp. 485-490
Author(s):  
Jan Söderström ◽  
Conny Arnerlöv ◽  
Stefan O. Emdin ◽  
Bengt Lundgren ◽  
Göran Roos ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Dna Ploidy ◽  
S Phase ◽  
Phase Fraction ◽  
Histologic Grading

S Phase Fractions and DNA Ploidy as Prognostic Factors in Breast Cancer

1989 ◽  
pp. 43-47 ◽  
Author(s):  
G. E. Feichter ◽  
D. von Fournier ◽  
M. Kaufmann
Keyword(s):  
Breast Cancer ◽  
Prognostic Factors ◽  
Dna Ploidy ◽  
S Phase

scholarly journals DNA ploidy and S-phase fraction as prognostic factors in patients with uveal melanomas

1995 ◽  
Vol 71 (1) ◽  
pp. 177-181 ◽  
Author(s):  
M Karlsson ◽  
B Boeryd ◽  
J Carstensen ◽  
B Kågedal ◽  
S Wingren
Keyword(s):  
Prognostic Factors ◽  
Dna Ploidy ◽  
S Phase ◽  
Phase Fraction ◽  
Uveal Melanomas

Relationship of DNA Ploidy and S-Phase Fraction to Survival After First Recurrence of Breast Cancer

1994 ◽  
Vol 33 (4) ◽  
pp. 423-429 ◽  
Author(s):  
Olle Stal ◽  
John M. Carstensen ◽  
Sten Wingren ◽  
Lars Erik Rutqvist ◽  
Lambert Skoog ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Dna Ploidy ◽  
S Phase ◽  
Phase Fraction ◽  
First Recurrence ◽  
Relationship Of

scholarly journals Evaluation of Prognostic Factors Following Flow-Cytometric DNA Analysis after Cytokeratin Labelling: II. Cervical and Endometrial Cancer

2002 ◽  
Vol 24 (4-5) ◽  
pp. 147-158 ◽  
Author(s):  
Pauline Wimberger ◽  
Peter Hillemanns ◽  
Thomas Kapsner ◽  
Hermann Hepp ◽  
Rainer Kimmig
Keyword(s):  
Cervical Cancer ◽  
Endometrial Cancer ◽  
Prognostic Factors ◽  
Endometrial Carcinoma ◽  
Dna Ploidy ◽  
S Phase ◽  
Phase Fraction ◽  
Dna Aneuploidy ◽  
Flow Cytometric

In gynecologic oncology valid prognostic factors are necessary to define biologically similar subgroups for analysis of therapeutic efficacy. This study is the first published prospective study concerning prognostic significance of DNA ploidy and S‐phase fraction in cervical and endometrial cancer following enrichment of tumor cells by cytokeratin labelling. Epithelial cells were labeled by FITC‐conjugated cytokeratin antibody (CK 5, 6, 8, and CK 17) prior to flow cytometric cell cycle analysis in 91 specimens of cervical cancer and 73 samples of endometrial cancer. In cervical cancer neither DNA‐ploidy nor S‐phase fraction were relevant prognostic parameters. But CV of the G0G1‐peak showed prognostic relevance in cervical cancer cells, even in multivariate analysis. This interesting observation, however, seems to have no therapeutic consequence due to the small discrimination capacity of CV. In endometrial carcinoma, gross DNA‐aneuploidy (DNA‐index > 1.3) and a high percentage of proliferating cells (>75th percentile) were univariate and multivariate highly significant prognostic factors for recurrence‐free survival. Especially DNA‐aneuploidy (DI>1.3) is one of the most important independent molecular biological prognostic factors. While diagnostic curettage we could identify risk patients even preoperatively by determination of the prognostic factors like histologic tumor type, grading, cervical involvement and DNA‐ploidy. Thereby these patients could be treated primarily in an oncologic center. In conclusion, our investigations showed that the determination of DNA‐ploidy should be done in endometrial carcinoma. In cervical cancer no clinical significance for determination of DNA‐parameters was found.

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