Distribution of vimentin-type intermediate filaments in Sertoli cells of the human testis, normal and pathologic

1988 ◽  
Vol 178 (2) ◽  
pp. 129-136 ◽  
Author(s):  
Gerhard Aumüller ◽  
Manfred Steinbrück ◽  
Walter Krause ◽  
Hans-Joachim Wagner
Keyword(s):  
Intermediate Filaments ◽  
Sertoli Cells ◽  
Human Testis ◽  
Type Intermediate

Expression of fetal-type intermediate filaments by 17-day-old rat Sertoli cells cultured on reconstituted basement membrane

1990 ◽  
Vol 260 (2) ◽  
pp. 395-401 ◽  
Author(s):  
Florian Guillou ◽  
Catherine Monet-Kuntz ◽  
Isabelle Fontaine ◽  
Jacques E. Flechon
Keyword(s):  
Basement Membrane ◽  
Intermediate Filaments ◽  
Sertoli Cells ◽  
Cells Cultured ◽  
Type Intermediate

Intermediate filaments in the Sertoli cells of the ageing human testis

1997 ◽  
Vol 431 (2) ◽  
pp. 131-138 ◽  
Author(s):  
Mar�a P. de Miguel ◽  
Ferm�n R. Bethencourt ◽  
M. I. Arenas ◽  
Benito Fraile ◽  
R. Paniagua
Keyword(s):  
Intermediate Filaments ◽  
Sertoli Cells ◽  
Human Testis

An unusual familial cardiomyopathy characterized by aberrant accumulations of desmin-type intermediate filaments

1981 ◽  
Vol 393 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Marie-Elisabeth Stoeckel ◽  
Mary Osborn ◽  
Aim� Porte ◽  
Andr� Sacrez ◽  
Andr� Batzenschlager ◽  
...  
Keyword(s):  
Intermediate Filaments ◽  
Familial Cardiomyopathy ◽  
Type Intermediate

scholarly journals Exogenous Gonadotrophin Stimulation Induces Partial Maturation of Human Sertoli Cells in a Testicular Xenotransplantation Model for Fertility Preservation

2020 ◽  
Vol 9 (1) ◽  
pp. 266 ◽  
Author(s):  
Marsida Hutka ◽  
Lee B. Smith ◽  
Ellen Goossens ◽  
W. Hamish B. Wallace ◽  
Jan-Bernd Stukenborg ◽  
...  
Keyword(s):  
Sertoli Cell ◽  
Sertoli Cells ◽  
Connexin 43 ◽  
Xenograft Model ◽  
Cell Maturation ◽  
Human Testis ◽  
Testicular Development ◽  
Future Fertility ◽  
And Function ◽  
Testis Tissue

The future fertility of prepubertal boys with cancer may be irreversibly compromised by chemotherapy and/or radiotherapy. Successful spermatogenesis has not been achieved following the xenotransplantation of prepubertal human testis tissue, which is likely due to the failure of somatic cell maturation and function. We used a validated xenograft model to identify the factors required for Leydig and Sertoli cell development and function in immature human testis. Importantly, we compared the maturation status of Sertoli cells in xenografts with that of human testis tissues (n = 9, 1 year-adult). Human fetal testis (n = 6; 14–21 gestational weeks) tissue, which models many aspects of prepubertal testicular development, was transplanted subcutaneously into castrated immunocompromised mice for ~12 months. The mice received exogenous human chorionic gonadotropin (hCG; 20IU, 3×/week). In xenografts exposed continuously to hCG, we demonstrate the maintenance of Leydig cell steroidogenesis, the acquisition of features of Sertoli cell maturation (androgen receptor, lumen development), and the formation of the blood–testis barrier (connexin 43), none of which were present prior to the transplantation or in xenografts in which hCG was withdrawn after 7 months. These studies provide evidence that hCG plays a role in Sertoli cell maturation, which is relevant for future investigations, helping them generate functional gametes from immature testis tissue for clinical application.

scholarly journals Familial desminopathy: myopathy with accumulation of desmin-type intermediate filaments.

1993 ◽  
Vol 56 (6) ◽  
pp. 644-648 ◽  
Author(s):  
J Vajsar ◽  
L E Becker ◽  
R M Freedom ◽  
E G Murphy
Keyword(s):  
Intermediate Filaments ◽  
Type Intermediate

scholarly journals Ontogenetic Pattern of Thyroid Hormone Receptor Expression in the Human Testis

2000 ◽  
Vol 85 (9) ◽  
pp. 3453-3457 ◽  
Author(s):  
Emmanuele A. Jannini ◽  
Anna Crescenzi ◽  
Nadia Rucci ◽  
Emiliano Screponi ◽  
Eleonora Carosa ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Thyroid Hormone ◽  
Sertoli Cells ◽  
Pcr Amplification ◽  
Receptor Expression ◽  
Northern Analysis ◽  
Human Testis ◽  
Adult Testis ◽  
Maximal Expression

Abstract We studied the spatiotemporal distribution of thyroid hormone nuclear receptors (TRs) α1 and α2 and β messenger RNA (mRNA) levels in normal human testicular tissue during development and in adulthood. Nonpathological specimens from five aborted fetuses (17 and 23 weeks of gestation, three and two cases, respectively) and from four patients undergoing orchiectomy (18 months old and 38-, 42-, and 52-yr-old, respectively) were analyzed by Northern blot, semiquantitative RT-PCR amplification using DNA sequences or specifically designed primers for the TR isoforms, and in situ hybridization. By using PCR amplification, we found that TRα1 and TRα2 are both expressed at different levels in fetal and adult testis. At all ages TRα2 is found at higher levels. Northern analysis showed hybridization signals corresponding to the expression of TRα2 and TRα1 in a ratio that increased from 2.6 at 17 weeks of gestation to 12.0 in adulthood. In fact, the expression of TRα1 dramatically decreased throughout development, being faintly detectable in the adult testis. Expression of TRβ was not detected at any age studied. This finding was further confirmed by PCR, which did not amplify TRβ either in fetal or in adult testis mRNAs. In situ hybridization studies showed the absence of TRβ and that TRα1 and TRα2 colocalized in Sertoli cells of prepubertal testis, whereas germ and interstitial cells appeared devoid of TR mRNA signals. From these results it can be concluded that the human testis exclusively expresses TRα, which is localized in Sertoli cells, TRβ being always undetectable. Fetal and prepubertal ages represent the period of maximal expression of TRα1 and TRα2. Theα 2/α1 ratio rises dramatically after development. These results confirm a critical window for the action of thyroid hormone in human testis, in the period of maximal expression of T3 binding isoform TRα1, and may account for the macroorchidism without virilization occurring when hyposecretion of thyroid hormones occurs before puberty.

scholarly journals Bronchial carcinoid cells contain neural-type intermediate filaments

Cancer ◽  
1984 ◽  
Vol 54 (4) ◽  
pp. 624-628 ◽  
Author(s):  
V.-P. Lehto ◽  
M. Miettinen ◽  
D. Dahl ◽  
I. Virtanen
Keyword(s):  
Intermediate Filaments ◽  
Bronchial Carcinoid ◽  
Type Intermediate

Expression of Glial and Vimentin Type Intermediate Filaments in Cultures Derived from Human Glial Material

1981 ◽  
Vol 19 (1-3) ◽  
pp. 161-167 ◽  
Author(s):  
MARY OSBORN ◽  
MONIKA LUDWIG-FESTL ◽  
KLAUS WEBER ◽  
AMICO BIGNAMI ◽  
DORIS DAHL ◽  
...  
Keyword(s):  
Intermediate Filaments ◽  
Type Intermediate
Sign in / Sign up

Export Citation Format

Share Document