Expression of fetal-type intermediate filaments by 17-day-old rat Sertoli cells cultured on reconstituted basement membrane

1990 ◽  
Vol 260 (2) ◽  
pp. 395-401 ◽  
Author(s):  
Florian Guillou ◽  
Catherine Monet-Kuntz ◽  
Isabelle Fontaine ◽  
Jacques E. Flechon
Keyword(s):  
Basement Membrane ◽  
Intermediate Filaments ◽  
Sertoli Cells ◽  
Cells Cultured ◽  
Type Intermediate

Distribution of vimentin-type intermediate filaments in Sertoli cells of the human testis, normal and pathologic

1988 ◽  
Vol 178 (2) ◽  
pp. 129-136 ◽  
Author(s):  
Gerhard Aumüller ◽  
Manfred Steinbrück ◽  
Walter Krause ◽  
Hans-Joachim Wagner
Keyword(s):  
Intermediate Filaments ◽  
Sertoli Cells ◽  
Human Testis ◽  
Type Intermediate

An unusual familial cardiomyopathy characterized by aberrant accumulations of desmin-type intermediate filaments

1981 ◽  
Vol 393 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Marie-Elisabeth Stoeckel ◽  
Mary Osborn ◽  
Aim� Porte ◽  
Andr� Sacrez ◽  
Andr� Batzenschlager ◽  
...  
Keyword(s):  
Intermediate Filaments ◽  
Familial Cardiomyopathy ◽  
Type Intermediate

Matrigel induces thymosin beta 4 gene in differentiating endothelial cells

1995 ◽  
Vol 108 (12) ◽  
pp. 3685-3694 ◽  
Author(s):  
D.S. Grant ◽  
J.L. Kinsella ◽  
M.C. Kibbey ◽  
S. LaFlamme ◽  
P.D. Burbelo ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Basement Membrane ◽  
Morphological Differentiation ◽  
Tube Formation ◽  
Vessel Formation ◽  
Thymosin Beta 4 ◽  
Cdna Hybridization ◽  
Matrix Components ◽  
Cells Cultured

We performed differential cDNA hybridization using RNA from endothelial cells cultured for 4 hours on either plastic or basement membrane matrix (Matrigel), and identified early genes induced during the morphological differentiation into capillary-like tubes. The mRNA for one clone, thymosin beta 4, was increased 5-fold. Immunostaining localized thymosin beta 4 in vivo in both growing and mature vessels as well as in other tissues. Endothelial cells transfected with thymosin beta 4 showed an increased rate of attachment and spreading on matrix components, and an accelerated rate of tube formation on Matrigel. An antisense oligo to thymosin beta 4 inhibited tube formation on Matrigel. The results suggest that thymosin beta 4 is induced and likely involved in differentiating endothelial cells. Thymosin beta 4 may play a role in vessel formation in vivo.

scholarly journals Culture patterns and sorting of rat Sertoli cell secretory proteins

1988 ◽  
Vol 89 (2) ◽  
pp. 175-188
Author(s):  
H. Ueda ◽  
L.L. Tres ◽  
A.L. Kierszenbaum
Keyword(s):  
Epithelial Cells ◽  
Sertoli Cell ◽  
Sertoli Cells ◽  
Secretory Proteins ◽  
Spermatogenic Cells ◽  
Squamous Cells ◽  
Nylon Mesh ◽  
Continuous Sheet ◽  
Peritubular Cells ◽  
Cells Cultured

A cocultivation chamber and two types of permeable substrates have been used to study: (1) the culture patterns of rat Sertoli and peritubular cells, and Sertoli cells cocultured with spermatogenic cells or peritubular cells; and (2) the polarized secretion of Sertoli cell-specific proteins transferrin, S70 and S45-S35 heterodimeric protein. Substrates included a nylon mesh (with openings of 100 micron) coated with extracellular matrix (ECM) material and an uncoated microporous filter (with pores of 0.45 micron). Sertoli cells cultured on ECM-coated nylon mesh organized a continuous sheet of multilayered epithelial cells essentially devoid of spermatogenic cells while peritubular cells formed a layer of squamous cells. Sertoli cells cultured on uncoated microporous substrate formed a continuous sheet of cuboidal epithelial cells with numerous basal cytoplasmic processes projecting into the substrate and abundant apically located spermatogenic cells, while peritubular cells organized one or two layers of loose squamous cells. [35S]methionine-labelled secretory proteins resolved by two-dimensional polyacrylamide gel electrophoresis and autoradiography displayed cell-specific patterns that were slightly influenced by the type of substrate. Sertoli cells cocultured with peritubular cells on uncoated microporous substrate under conditions that enabled separation of apical and basal surfaces, secreted proteins in a polarized fashion. While transferrin was released bidirectionally, S45-S35 heterodimeric protein was released apically. S70 was detected in both apical and basal compartments. We conclude from these studies that: (1) the number of spermatogenic cells decreases when Sertoli-spermatogenic cell cocultures are prepared on ECM-coated nylon substrate; and (2) Sertoli cells in coculture with spermatogenic or peritubular cells on uncoated microporous substrate, organize continuous sheets displaying polarized protein secretion.

Exposure to Pb and Cd alters MCT4/CD147 expression and MCT4/CD147-dependent lactate transport in mice Sertoli cells cultured in vitro

2019 ◽  
Vol 56 ◽  
pp. 30-40 ◽  
Author(s):  
Jun Yu ◽  
Jiantao Sun ◽  
Yongsheng Fan ◽  
Jianmei Su ◽  
Jie Xie ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Lactate Transport ◽  
Cd147 Expression ◽  
Cells Cultured
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