An optimal time control problem for the administration of 2′,3′-dideoxycytidine by using red blood cells as bioreactors

1993 ◽  
Vol 55 (4) ◽  
pp. 715-730
Author(s):  
E. Beretta ◽  
F. Solimano
Keyword(s):  
Control Problem ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Optimal Time ◽  
Time Control

CYTOMORPHOLOGY VAGINAL SMEARS OF DOGS IN DIFFERENT PERIODS OF THE SEXUAL CYCLE

2016 ◽  
Vol 1 (4) ◽  
pp. 86-89
Author(s):  
Гришина ◽  
Darya Grishina ◽  
Минюк ◽  
Lyudmila Minyuk
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
White Blood Cells ◽  
Sexual Cycle ◽  
Optimal Time ◽  
Minor Amount ◽  
Vaginal Smear ◽  
Basal Cells ◽  
Unit Cells ◽  
Intermediate Cells

The purpose of work is improving the efficiency of fertilization dog breed French Bulldog. The results of cytological examination of vaginal mucus in dogs at different periods of the sexual cycle. This will determine the optimal time for insemination. As a result of vaginal smear cytology of dogs among the signs of days favorable for breeding are the days when cytological smears appear more than 60% of the surface of pycnotic cells, a small amount of red blood cells and white blood cells, superficial cells are arranged in groups of 4-6 cells or clusters in the form of tiles. Proestrus phase corresponds to the presence of only of basal cells in a smear at the beginning. Then trend towards a decrease of intermediate and superficial cell growth. In the smears are found red blood cells and white blood cells. The duration of the stage of 6-8 days. Estrus phase is characterized by the appearance in the smear of more than 60% of the surface of pycnotic cells, a minor amount of red blood cells and white blood cells. The duration of the stage of 10-11 days. It was found that in dogs the most optimal days for mating are 3-4 day estrus. A smear diestrus phase accompanied by the presence of abrupt changes in the cellular structure. The picture a smear vaginal mucus in diestrus are signs are reducing the number of superficial cells, and increasing the number of intermediate cells; changes in the nature and appearance leukocyte, smear of mucus becomes turbid, dusky background. The duration of stage about 60 days. In anestrus cytological picture of the mucous membrane is stable. There are small cell in smears, revealed basal epithelial cells, can occur intermediate unit cells and leukocytes. The duration of the stage about 123 days.

Hemoglobin crystals of the red blood cells in the human renal cortex: electron microscopic observations of the renal lithiasis

1978 ◽  
Vol 36 (2) ◽  
pp. 480-481
Author(s):  
Kosuke Ueda ◽  
Hiroto Washida ◽  
Nakazo Watari
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Renal Cortex ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Renal Lithiasis ◽  
Electron Microscopic ◽  
Hemoglobin C ◽  
First Case ◽  
Ultrathin Sections

IntroductionHemoglobin crystals in the red blood cells were electronmicroscopically reported by Fawcett in the cat myocardium. In the human, Lessin revealed crystal-containing cells in the periphral blood of hemoglobin C disease patients. We found the hemoglobin crystals and its agglutination in the erythrocytes in the renal cortex of the human renal lithiasis, and these patients had no hematological abnormalities or other diseases out of the renal lithiasis. Hemoglobin crystals in the human erythrocytes were confirmed to be the first case in the kidney.Material and MethodsTen cases of the human renal biopsies were performed on the operations of the seven pyelolithotomies and three ureterolithotomies. The each specimens were primarily fixed in cacodylate buffered 3. 0% glutaraldehyde and post fixed in osmic acid, dehydrated in graded concentrations of ethanol, and then embedded in Epon 812. Ultrathin sections, cut on LKB microtome, were doubly stained with uranyl acetate and lead citrate.

Densitometric Identification of Primitive Erythroblasts After Reaction With Diaminobenzidine

1973 ◽  
Vol 31 ◽  
pp. 328-329
Author(s):  
John A. Trotter
Keyword(s):  
Red Blood Cells ◽  
Analytical Method ◽  
Blood Cells ◽  
Guinea Pigs ◽  
Specific Protein ◽  
Visual Examination ◽  
Hemoglobin Synthesis ◽  
Peroxidatic Activity ◽  
Small Density

Hemoglobin is the specific protein of red blood cells. Those cells in which hemoglobin synthesis is initiated are the earliest cells that can presently be considered to be committed to erythropoiesis. In order to identify such early cells electron microscopically, we have made use of the peroxidatic activity of hemoglobin by reacting the marrow of erythropoietically stimulated guinea pigs with diaminobenzidine (DAB). The reaction product appeared as a diffuse and amorphous electron opacity throughout the cytoplasm of reactive cells. The detection of small density increases of such a diffuse nature required an analytical method more sensitive and reliable than the visual examination of micrographs. A procedure was therefore devised for the evaluation of micrographs (negatives) with a densitometer (Weston Photographic Analyzer).

Sign in / Sign up

Export Citation Format

Share Document