Adaptation to physical exercise increases expression of Ca-ATPase gene in myocardial sarcoplasmic reticulum

1999 ◽  
Vol 128 (1) ◽  
pp. 677-680 ◽  
Author(s):  
M. G. Pshennikova ◽  
G. L. Khaspekov ◽  
A. O. Tatarenko ◽  
I. Yu. Malyshev ◽  
R. Sh. Bibilashvili
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Physical Exercise ◽  
Adaptation To Physical Exercise ◽  
Atpase Gene

Skeletal Muscle Sarcoplasmic Reticulum Ca 2+ -ATPase Gene Expression in Congestive Heart Failure

1997 ◽  
Vol 81 (5) ◽  
pp. 703-710 ◽  
Author(s):  
David G. Peters ◽  
Heather L. Mitchell ◽  
Sylvia A. McCune ◽  
Sonhee Park ◽  
Jay H. Williams ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heart Failure ◽  
Skeletal Muscle ◽  
Congestive Heart Failure ◽  
Sarcoplasmic Reticulum ◽  
Atpase Gene

Phorbol 12-myristate 13-acetate alters SR Ca(2+)-ATPase gene expression in cultured neonatal rat heart cells

1996 ◽  
Vol 271 (3) ◽  
pp. H1031-H1039 ◽  
Author(s):  
M. Qi ◽  
J. W. Bassani ◽  
D. M. Bers ◽  
A. M. Samarel
Keyword(s):  
Gene Expression ◽  
Sarcoplasmic Reticulum ◽  
Mrna Stability ◽  
Primary Cultures ◽  
Neonatal Rat ◽  
Response To Treatment ◽  
Similar Degree ◽  
Heart Cells ◽  
Mrna Levels ◽  
Atpase Gene

Primary cultures of neonatal rat ventricular myocytes were used to examine how the cardiac myocyte cytoplasmic Ca2+ ([Ca2+]i) transient and sarcoplasmic reticulum Ca(2+)-ATPase (SERCA2) gene expression change in response to treatment with the protein kinase C activator phorbol 12-myristate 13-acetate (PMA). Exposure of neonatal myocytes to PMA (200 nM, 48-72 h) produced myocyte growth and a 70% prolongation of the half-time for [Ca2+]i decline induced by potassium depolarization in the absence of extracellular Na+ (in which the sarcoplasmic reticulum Ca2+ pump is the main mechanism responsible for [Ca2+]i decline). The reduced rate of [Ca2+]i transient decline corresponded to a 53% reduction in SERCA2 protein levels and a 43% reduction in SERCA2 mRNA levels as compared with control myocytes. Exposure to PMA for as little as 30 min or for as long as 48 h produced a similar degree of SERCA2 mRNA downregulation over time. PMA-induced downregulation of SERCA2 mRNA levels was blocked by either 10 nM staurosporine or 4 microM chelerythrine, whereas treatment with either agent alone increased SERCA2 mRNA levels as compared with control cells. Actinomycin D mRNA stability assays revealed that PMA treatment appeared to markedly destabilize the relatively long-lived SERCA2 mRNA transcript. Taken together, these results indicate that downregulation of SERCA2 gene by PMA in cultured neonatal myocytes occurs at least in part by alterations in mRNA stability and results in functional alterations in [Ca2+]i decline that are similar to that observed in the hypertrophied and failing adult myocardium.

Enhanced expression of sarcoplasmic reticulum Ca2+-ATPase gene plays a role in protective effects of nitric oxide

1999 ◽  
Vol 128 (4) ◽  
pp. 981-984
Author(s):  
N. P. Aimasheva ◽  
E. B. Malenyuk ◽  
E. B. Manukhina ◽  
G. L. Khaspekov ◽  
V. D. Mikoyan ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Sarcoplasmic Reticulum ◽  
Protective Effects ◽  
Atpase Gene ◽  
Enhanced Expression

scholarly journals Electrical stimulation of C2C12 myotubes induces contractions and represses thyroid-hormone-dependent transcription of the fast-type sarcoplasmic-reticulum Ca2+-ATPase gene

1997 ◽  
Vol 321 (3) ◽  
pp. 845-848 ◽  
Author(s):  
Marc H. M. THELEN ◽  
Warner S. SIMONIDES ◽  
Cornelis van HARDEVELD
Keyword(s):  
Electrical Stimulation ◽  
Thyroid Hormone ◽  
Sarcoplasmic Reticulum ◽  
Contractile Activity ◽  
Motor Nerve ◽  
C2c12 Myotubes ◽  
Transcriptional Level ◽  
Regulation Of Expression ◽  
Atpase Gene ◽  
Stimulation Of

Chronic low-frequency contraction of skeletal muscle, either induced by a slow motor nerve or through direct electrical stimulation, generally induces expression of proteins associated with the slow phenotype, while repressing the corresponding fast isoforms. Contractions thereby counteract the primarily transcriptional effect of thyroid hormone (T3), which results in the selective induction and stimulation of expression of fast isoforms. We studied the regulation of expression of the fast-type sarcoplasmic-reticulum Ca2+-ATPase (SERCA1), a characteristic component of the fast phenotype. Previous work suggested that reduction of SERCA1 expression by contractile activity might result from interference with the T3-dependent transcriptional stimulation of the SERCA1 gene. The present study was set up to test this unexpected mode of action of contractile activity. We show that electrical stimulation of C2C12 mouse myotubes, which results in synchronous contractions at the imposed frequency, reduces basal but virtually abolishes T3-dependent SERCA1 expression. T3-dependent expression of a reporter gene driven by the SERCA1 promoter was similarly affected by electrical stimulation. This is the first demonstration that the counteracting effects on muscle gene expression of electrically induced contractions and T3 may interact at the transcriptional level.

Plasma-coagulation hemostasis in physically active subjects during adaptation to physical exercise

2007 ◽  
Vol 33 (6) ◽  
pp. 736-741 ◽  
Author(s):  
S. V. Tikhomirova ◽  
A. D. Vikulov ◽  
A. A. Baranov ◽  
I. A. Osetrov
Keyword(s):  
Physical Exercise ◽  
Physically Active ◽  
Plasma Coagulation ◽  
Adaptation To Physical Exercise
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