Hemopoietic microenvironment-transferring units and inducible hemopoietic stromal precursors in the bone marrow of thymectomized mice

1999 ◽  
Vol 127 (2) ◽  
pp. 169-171
Author(s):  
T. V. Todriya
Keyword(s):  
Bone Marrow ◽  
Stromal Precursors ◽  
Hemopoietic Microenvironment ◽  
Thymectomized Mice

Effects of adrenal glands on bone marrow hemopoietic microenvironment

1999 ◽  
Vol 128 (5) ◽  
pp. 1169-1173
Author(s):  
I. A. Khlusov ◽  
T. Yu. Raskovalova ◽  
E. V. Kirienkova ◽  
A. M. Dygai
Keyword(s):  
Bone Marrow ◽  
Adrenal Glands ◽  
Hemopoietic Microenvironment

scholarly journals CD34 expression by stromal precursors in normal human adult bone marrow

Blood ◽  
1991 ◽  
Vol 78 (11) ◽  
pp. 2848-2853 ◽  
Author(s):  
PJ Simmons ◽  
B Torok-Storb
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Complex Mixture ◽  
Antigen Expression ◽  
Cell Types ◽  
Stromal Precursors ◽  
Cd34 Antigen ◽  
Distinct Cell ◽  
Marrow Cells

Normal bone marrow cells were isolated by fluorescence-activated cell sorting (FACS) on the basis of CD34 antigen expression and then assayed in vitro for colonies of fibroblastic cells (fibroblast colony-forming units [CFU-F]). Greater than 95% of detectable CFU-F were recovered in the CD34+ population, while their numbers were markedly depleted in the CD34- population. Additional experiments showed that the majority of CFU-F exhibited high forward and perpendicular light scatter and low- density CD34 antigen. Growth of sorted cells in medium optimized for long-term marrow culture (LTMC) produced a complex mixture of adherent stromal elements including fibroblasts, adipocytes, smooth muscle cells, and macrophages. Monoclonal antibody STRO-1, which identifies bone marrow stromal cells, reacted with approximately 5% of CD34+ cells, which included all CFU-F and stromal precursors in LTMC. Experiments using soybean agglutinin (SBA) further showed that these stromal elements were restricted to a population of bone marrow cells with the phenotype CD34+/SBA+. These properties of stromal precursors are quite distinct from those of primitive hematopoietic progenitors, showing that although the precursors of the hematopoietic and stromal systems share expression of CD34, they are otherwise phenotypically distinct cell types.

scholarly journals The Effects of Allografts of Thymic Epithelial Reticular Cells on the Lymphoid Tissues of Neonatally Thymectomized Mice

Blood ◽  
1967 ◽  
Vol 29 (1) ◽  
pp. 29-40 ◽  
Author(s):  
ESTHER FINCHER HAYS
Keyword(s):  
Bone Marrow ◽  
Diffusion Chamber ◽  
Lymphoid Tissues ◽  
Lymphoma Cells ◽  
Cortical Cells ◽  
Normal Manner ◽  
C3h Mice ◽  
Reticular Cells ◽  
Thymectomized Mice

Abstract Neonatal thymus placed in a Millipore diffusion chamber for one week loses its cortical cells, while the epithelial reticular cells remain viable. Grafts of these remnants in neonatally thymectomized mice are completely reconstituted from cells of the thymectomized host. Evidence is presented which suggests that the bone marrow may be a source of cells which reform the thymus cortex. These remnant grafts result in lymphoid reconstitution of neonatally thymectomized mice. The grafted animals also reject allografts of skin and lymphoma cells in a normal manner. However, these C3H mice, neonatally thymectomized and reconstituted with allografts of AKR thymic epithelial reticular cells, are tolerant to grafts of AKR lymphoma cells.

scholarly journals Studies of the Hemopoietic Microenvironment. I. Changes in the Microvascular System and Stroma During Erythropoietic Regeneration and Suppression in the Spleens of CF1 Mice

Blood ◽  
1972 ◽  
Vol 39 (5) ◽  
pp. 697-712 ◽  
Author(s):  
Robert S. McCuskey ◽  
Howard A. Meineke ◽  
Samuel F. Townsend
Keyword(s):  
Bone Marrow ◽  
Blood Flow ◽  
Linear Velocity ◽  
Proliferating Cells ◽  
Hemopoietic Microenvironment ◽  
Murine Spleen ◽  
Flow Through ◽  
Tissue Compartments ◽  
Red Pulp

Abstract Specific alterations in the microvascular and connective tissue compartments of the hemopoietic microenvironment have been examined during erythropoietic regeneration and suppression in the murine spleen and bone marrow using in vivo microscopic and histochemical methods. The results have confirmed the concept of specific hemopoietic microenvironments and have demonstrated specific alterations in the microenvironment during erythropoietic stimulation and repression. Elevated erythropoiesis in the splenic red pulp is accompanied by an elevation in blood flow through the microvascular system. Both the linear velocity of flow and the number of sinusoids with blood flow in them increased significantly. In contrast, erythropoietic repression was accompanied by a decreased linear velocity of blood flow, as well as a marked increase in the amount of blood being stored in the splenic sinusoids. This also was the picture when diffuse granulopoiesis was present in the red pulp, or when granuloid or undifferentiated colonies were present. The chemical composition of the stroma in the spleen and bone marrow also varied during states of hemopoietic activity and, in addition, there were differences in the composition of the stroma between these two organs. In both organs, foci of early proliferating cells were enveloped by a coating of sulfated acid mucopolysaccharide. This coat persisted on cells in later stages of granulopoiesis but not on cells in the later stages of erythropoiesis. The latter were enveloped with a coating of neutral mucopolysaccharide. A tentative hypothesis to explain the mechanisms involved in producing these changes is discussed.

scholarly journals Differential sensitivity to cryopreservation of clonogenic progenitor cells and stromal precursors from leukemic and normal bone marrow

Stem Cells ◽  
1994 ◽  
Vol 12 (2) ◽  
pp. 180-186 ◽  
Author(s):  
H. A. Zaheer ◽  
F. M. Gibson ◽  
M. Bagnara ◽  
E. C. Gordon-Smith ◽  
T. R. Rutherford
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Differential Sensitivity ◽  
Normal Bone Marrow ◽  
Stromal Precursors ◽  
Normal Bone

scholarly journals CD34 expression by stromal precursors in normal human adult bone marrow

Blood ◽  
1991 ◽  
Vol 78 (11) ◽  
pp. 2848-2853 ◽  
Author(s):  
PJ Simmons ◽  
B Torok-Storb
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Complex Mixture ◽  
Antigen Expression ◽  
Cell Types ◽  
Stromal Precursors ◽  
Cd34 Antigen ◽  
Distinct Cell ◽  
Marrow Cells

Abstract Normal bone marrow cells were isolated by fluorescence-activated cell sorting (FACS) on the basis of CD34 antigen expression and then assayed in vitro for colonies of fibroblastic cells (fibroblast colony-forming units [CFU-F]). Greater than 95% of detectable CFU-F were recovered in the CD34+ population, while their numbers were markedly depleted in the CD34- population. Additional experiments showed that the majority of CFU-F exhibited high forward and perpendicular light scatter and low- density CD34 antigen. Growth of sorted cells in medium optimized for long-term marrow culture (LTMC) produced a complex mixture of adherent stromal elements including fibroblasts, adipocytes, smooth muscle cells, and macrophages. Monoclonal antibody STRO-1, which identifies bone marrow stromal cells, reacted with approximately 5% of CD34+ cells, which included all CFU-F and stromal precursors in LTMC. Experiments using soybean agglutinin (SBA) further showed that these stromal elements were restricted to a population of bone marrow cells with the phenotype CD34+/SBA+. These properties of stromal precursors are quite distinct from those of primitive hematopoietic progenitors, showing that although the precursors of the hematopoietic and stromal systems share expression of CD34, they are otherwise phenotypically distinct cell types.

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