Uptake of collagenolytic enzymes by bone cells during isolation from embryonic rat calvaria

1981 ◽  
Vol 33 (1) ◽  
pp. 255-260 ◽  
Author(s):  
Jaro Sodek ◽  
Johan N. M. Heersche
Keyword(s):  
Bone Cells ◽  
Rat Calvaria

scholarly journals Effect of various media on mineralization and alkaline phosphatase activity in bone cells from embryonic rat calvaria

1995 ◽  
Vol 67 ◽  
pp. 241
Author(s):  
Yoshitaka Yoshimura ◽  
Yoh Hisada ◽  
Kuniaki Suzuki ◽  
Yosbiaki Deyama ◽  
Akira Matsumoto
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Bone Cells ◽  
Rat Calvaria

Membrane potential of rat calvaria bone cells: Dependence on temperature

1990 ◽  
Vol 144 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Ruth Massas ◽  
Rafi Korenstein ◽  
Dieter Bincmann ◽  
Peter Tetsch
Keyword(s):  
Membrane Potential ◽  
Bone Cells ◽  
Rat Calvaria

Cell behaviour of rat calvaria bone cells on surfaces with random nanometric features

2003 ◽  
Vol 23 (3) ◽  
pp. 337-340 ◽  
Author(s):  
M.O. Riehle ◽  
M.J. Dalby ◽  
H. Johnstone ◽  
A. MacIntosh ◽  
S. Affrossman
Keyword(s):  
Bone Cells ◽  
Cell Behaviour ◽  
Rat Calvaria

Production of Calcification Inhibitors by Rat Calvaria and Bone Cells in Culture

1988 ◽  
Vol &NA; (232) ◽  
pp. 119???126
Author(s):  
KEIICHI OHYA ◽  
JOHN L. MEYER ◽  
ROLF FELIX ◽  
HERBERT FLEISCH
Keyword(s):  
Bone Cells ◽  
Cells In Culture ◽  
Rat Calvaria ◽  
Calcification Inhibitors

Aerobic glycolysis in bone: lactic acid production by rat calvaria cells in culture

1978 ◽  
Vol 234 (1) ◽  
pp. C51-C55 ◽  
Author(s):  
R. Felix ◽  
W. F. Neuman ◽  
H. Fleisch
Keyword(s):  
Parathyroid Hormone ◽  
Bone Cells ◽  
Aerobic Glycolysis ◽  
Lactic Acid Production ◽  
Lactate Production ◽  
Isolated Cells ◽  
Rat Calvaria ◽  
Intact Bone ◽  
Log Linear ◽  
Higher Sensitivity

Considerable data have been accumulated on aerobic glycolysis of intact bone preparations. To test whether aerobic glycolysis is a feature of the bone cells themselves or of the localized conditions within an intact bone, cells isolated from rat calvaria were cultured and the effect of several factors on lactate production was determined. These cells drastically decreased lactate production when the pH in the culture medium was lowered, changing from 100 to 20 percent for a pH shift from 7.4 to 6.75. L-lactate inhibited its own formation by 40 percent at 20 mM. Parathyroid hormone (PTH) (820 U/mg) at a concentration ranging from 0.2 to 5.0 U/ml stimulated slightly the lactate production in a log-linear response, the ratio treated over control changing from 1.1 to 1.3. The maximal stimulation is observed at pH 7.0. Isolated cells respond qualitatively the same as did intact calvaria. Quantitatively, there were significant differences: notably, a smaller response to parathyroid hormone and a higher sensitivity to lowered pH.

The incidence and size of gap junctions between the bone cells in rat calvaria

1993 ◽  
Vol 187 (4) ◽  
Author(s):  
S.J. Jones ◽  
C. Gray ◽  
H. Sakamaki ◽  
M. Arora ◽  
A. Boyde ◽  
...  
Keyword(s):  
Gap Junctions ◽  
Bone Cells ◽  
Rat Calvaria

The Healing of Bone Defects by Cell-Free and Stem Cell-Seeded 3D Printed PLA Tissue Engineered Scaffolds

2021 ◽  
Author(s):  
Marjan Bahraminasab ◽  
Athar Talebi ◽  
Nesa Doostmohammadi ◽  
Samaneh Arab ◽  
Ali Ghanbari ◽  
...  
Keyword(s):  
Stem Cell ◽  
Bone Cells ◽  
Osteogenic Potential ◽  
Bone Maturation ◽  
Sem Images ◽  
Cell Scaffolds ◽  
Rat Calvaria ◽  
Bone Defect Healing ◽  
Defect Sites ◽  
3D Printed

Abstract One of the main issues in bone tissue engineering is to realize the response of the host to the engineered scaffolds. In this paper, the in-vivo healing of critical-sized bony defects by cell-free and stem cell-seeded 3D printed PLA scaffolds was studied in rat calvaria bone. First, the scaffolds were 3D printed based on a designed computer model and half of them were seeded by with bone marrow-derived mesenchymal stem cells (BMSCs). The SEM images of the surfaces of PLA and PLA+Cell scaffolds were taken for morphological analysis. All the scaffolds were implanted in the defect sites of rat calvaria bones and histological analysis was conducted after 8 and 12 weeks. The results showed that both cell-free and stem cell-seeded scaffolds exhibited superb healing compared with the empty defect controls. The histological observation revealed the formation of both new bone and connective tissues in the healing site after 8 and 12 weeks, postoperatively. The bone cells including osteoblasts and osteocytes with lacuna were also observed. The higher filled area and the higher bone formation and bone maturation were observed after 12 weeks and in particular for PLA+Cell scaffolds. Furthermore, the systemic toxicity evaluation of the scaffolds using ALT and AST tests reject any toxicity for both cell-free and stem cell-seeded scaffolds. It can be concluded that the 3D printed PLA scaffold with BMSCs seeding has well osteogenic potential to be used for bone defect healing.

Further studies on Percoll-separated bone cells from fetal rat calvaria

1983 ◽  
Vol 5 (3) ◽  
pp. 154
Author(s):  
I.P. Braidman ◽  
N. Virani ◽  
D.C. Anderson
Keyword(s):  
Bone Cells ◽  
Rat Calvaria ◽  
Fetal Rat

scholarly journals Stimulation by defined parathyroid hormone fragments of cell proliferation in skeletal-derived cell cultures

1990 ◽  
Vol 272 (3) ◽  
pp. 781-785 ◽  
Author(s):  
D Sömjen ◽  
I Binderman ◽  
K D Schlüter ◽  
E Wingender ◽  
H Mayer ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Cyclic Amp ◽  
Dna Synthesis ◽  
Cell Cultures ◽  
Thymidine Incorporation ◽  
Bone Cells ◽  
Functional Domain ◽  
Independent Manner ◽  
Rat Calvaria ◽  
Calvaria Cell

We have reported previously that parathyroid hormone (PTH) acts on cultured bone cells to stimulate creatine kinase (CK) activity and [3H]thymidine incorporation into DNA via phosphoinositide turnover, in addition to its other actions via increased cyclic AMP production. We also found that mid-region fragments of PTH stimulate [3H]thymidine incorporation into avian chondrocytes. In the present study of mammalian systems, we demonstrate differential effects of defined synthetic PTH fragments on CK activity and DNA synthesis, as compared with cyclic AMP production, in osteoblast-enriched embryonic rat calvaria cell cultures, in an osteoblast-like clone of rat osteosarcoma cells (ROS 17/2.8) and in chondroblasts from rat epiphysial cartilage cell cultures. Unlike full-length bovine (b)PTH-(1-84) or the fully effective shorter fragment human (h)PTH-(1-34), fragments lacking the N-terminal region of the hormone did not increase cyclic AMP formation, whereas they did stimulate increases in both DNA synthesis and CK activity. Moreover, the PTH fragment hPTH-(28-48) at 10 microM inhibited the increase in cyclic AMP caused by 10 nM-bPTH-(1-84). The increase of CK activity in ROS 17/2.8 cells caused by bPTH-(1-84) or hPTH-(28-48) was completely inhibited by either cycloheximide or actinomycin D, as was shown previously for rat calvaria cell cultures. These results indicated the presence of a functional domain of PTH in the central part of the molecule which exerts its mitogenic-related effects on osteoblast- and chondroblast-like cells in a cyclic AMP-independent manner. Since cyclic AMP formation by PTH leads to bone resorption, specific mid-region fragments of PTH might prove suitable for use in vivo to induce bone formation without concomitant resorption.

Pathology of Bone Cells in Pigs with Experimental Turbinate Osteoporosis (Atrophic Rhinitis)

1971 ◽  
Vol 29 ◽  
pp. 304-305
Author(s):  
A. W. Fetter ◽  
C. C. Capen
Keyword(s):  
Mucous Membrane ◽  
Bone Cells ◽  
Atrophic Rhinitis ◽  
Infectious Agents ◽  
Metabolic Disturbances ◽  
Progressive Reduction ◽  
Naturally Occurring ◽  
Fundamental Cause ◽  
Osteocytic Osteolysis ◽  
Uncertain Etiology

Atrophic rhinitis in swine is a disease of uncertain etiology in which infectious agents, hereditary predisposition, and metabolic disturbances have been reported to be of primary etiologic importance. It shares many similarities, both clinically and pathologically, with ozena in man. The disease is characterized by deformity and reduction in volume of the nasal turbinates. The fundamental cause for the localized lesion of bone in the nasal turbinates has not been established. Reduced osteogenesis, increased resorption related to inflammation of the nasal mucous membrane, and excessive resorption due to osteocytic osteolysis stimulated by hyperparathyroidism have been suggested as possible pathogenetic mechanisms.The objectives of this investigation were to evaluate ultrastructurally bone cells in the nasal turbinates of pigs with experimentally induced atrophic rhinitis, and to compare these findings to those in control pigs of the same age and pigs with the naturally occurring disease, in order to define the fundamental lesion responsible for the progressive reduction in volume of the osseous core.

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