Kinetics of uptake and metabolism by endothelial cell from indicator dilution data

1987 ◽  
Vol 15 (2) ◽  
pp. 201-215 ◽  
Author(s):  
John H. Linehan ◽  
Thomas A. Bronikowski ◽  
Christopher A. Dawson
Keyword(s):  
Endothelial Cell ◽  
Indicator Dilution ◽  
Kinetics Of Uptake ◽  
Kinetics Of ◽  
Uptake And Metabolism

Kinetics of Uptake and Metabolism of Atrazine in Model Plant Systems

1997 ◽  
Vol 49 (2) ◽  
pp. 157-163 ◽  
Author(s):  
Muriel Raveton ◽  
Patrick Ravanel ◽  
Anne Marie Serre ◽  
Françoise Nurit ◽  
Michel Tissut
Keyword(s):  
Model Plant ◽  
Kinetics Of Uptake ◽  
Kinetics Of ◽  
Uptake And Metabolism

scholarly journals Uptake and metabolism of β-apo-8′-carotenal, β-apo-10′-carotenal, and β-apo-13-carotenone in Caco-2 cells

2019 ◽  
Vol 60 (6) ◽  
pp. 1121-1135 ◽  
Author(s):  
Boluwatiwi O. Durojaye ◽  
Kenneth M. Riedl ◽  
Robert W. Curley ◽  
Earl H. Harrison
Keyword(s):  
Cell Monolayer ◽  
Intestinal Cells ◽  
Limited Information ◽  
Differentiated Cells ◽  
Minor Metabolite ◽  
Tween 40 ◽  
Kinetics Of Uptake ◽  
A Minor ◽  
Kinetics Of ◽  
Uptake And Metabolism

β-Apocarotenoids are eccentric cleavage products of carotenoids formed by chemical and enzymatic oxidations. They occur in foods containing carotenoids and thus might be directly absorbed from the diet. However, there is limited information about their intestinal absorption. The present research examined the kinetics of uptake and metabolism of β-apocarotenoids. Caco-2 cells were grown on 6-well plastic plates until a differentiated cell monolayer was achieved. β-Apocarotenoids were prepared in Tween 40 micelles, delivered to differentiated cells in serum-free medium, and incubated at 37°C for up to 8 h. There was rapid uptake of β-apo-8′-carotenal into cells, and β-apo-8′-carotenal was largely converted to β-apo-8′-carotenoic acid and a minor metabolite that we identified as 5,6-epoxy-β-apo-8′-carotenol. There was also rapid uptake of β-apo-10′-carotenal into cells, and β-apo-10′-carotenal was converted into a major metabolite identified as 5,6-epoxy-β-apo-10′-carotenol and a minor metabolite that is likely a dihydro-β-apo-10′-carotenol. Finally, there was rapid cellular uptake of β-apo-13-carotenone, and this compound was extensively degraded. These results suggest that dietary β-apocarotenals are extensively metabolized in intestinal cells via pathways similar to the metabolism of retinal. Thus, they are likely not absorbed directly from the diet.

scholarly journals Influence of fluorophore and linker length on the localization and trafficking of fluorescent sterol probes

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jarmila Králová ◽  
Michal Jurášek ◽  
Lucie Mikšátková ◽  
Anna Marešová ◽  
Jan Fähnrich ◽  
...  
Keyword(s):  
Genetic Mutation ◽  
Cell Permeability ◽  
Specific Cell ◽  
Cell Compartments ◽  
Intracellular Compartments ◽  
Resolution Imaging ◽  
Intracellular Labelling ◽  
Kinetics Of Uptake ◽  
Kinetics Of ◽  
Derivatives Of

AbstractFluorescent sterol probes, comprising a fluorophore connected to a sterol backbone by means of a linker, are promising tools for enabling high-resolution imaging of intracellular cholesterol. In this study, we evaluated how the size of the linker, site of its attachment and nature of the fluorophore, affect the localization and trafficking properties of fluorescent sterol probes. Varying lengths of linker using the same fluorophore affected cell penetration and retention in specific cell compartments. A C-4 linker was confirmed as optimal. Derivatives of heterocyclic sterol precursors attached with identical C-4 linker to different fluorophores at diverse positions also showed significant differences in their binding properties to various intracellular compartments and kinetics of trafficking. Two novel red-emitting probes with good cell permeability, fast intracellular labelling and slightly different distribution displayed very promising characteristics for sterol probes. These probes also strongly labelled endo/lysosomal compartment in cells with pharmacologically disrupted cholesterol transport, or with a genetic mutation of cholesterol transporting protein NPC1, that overlapped with filipin staining of cholesterol. Overall, the present study demonstrates that the physicochemical properties of the fluorophore/linker pairing determine the kinetics of uptake and distribution and subsequently influence the applicability of final probes.

scholarly journals Kinetics of L-Theanine Uptake and Metabolism in Healthy Participants Are Comparable after Ingestion of L-Theanine via Capsules and Green Tea

2012 ◽  
Vol 142 (12) ◽  
pp. 2091-2096 ◽  
Author(s):  
Lisa Scheid ◽  
Sabine Ellinger ◽  
Birgit Alteheld ◽  
Hannes Herholz ◽  
Jörg Ellinger ◽  
...  
Keyword(s):  
Green Tea ◽  
Kinetics Of ◽  
Uptake And Metabolism ◽  
Healthy Participants

Kinetics of uptake and deacetylation of N-acetylcysteine by human erythrocytes

2007 ◽  
Vol 39 (9) ◽  
pp. 1698-1706 ◽  
Author(s):  
Julia E. Raftos ◽  
Stephney Whillier ◽  
Bogdan E. Chapman ◽  
Philip W. Kuchel
Keyword(s):  
Human Erythrocytes ◽  
Kinetics Of Uptake ◽  
Kinetics Of

A note on the kinetics of uptake of d-glucose by the food yeast, Candida utilis

1976 ◽  
Vol 111 (1-2) ◽  
pp. 193-194 ◽  
Author(s):  
James A. Barnett ◽  
Anthony P. Sims
Keyword(s):  
Candida Utilis ◽  
Food Yeast ◽  
Kinetics Of Uptake ◽  
Kinetics Of

Kinetics of endothelial cell seeding

1985 ◽  
Vol 2 (6) ◽  
pp. 778-784 ◽  
Author(s):  
J. E. Rosenman ◽  
R. F. Kempczinski ◽  
W. H. Pearce ◽  
Edward B. Silberstein
Keyword(s):  
Endothelial Cell ◽  
Cell Seeding ◽  
Endothelial Cell Seeding ◽  
Kinetics Of
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