A note on the kinetics of uptake of d-glucose by the food yeast, Candida utilis

1976 ◽  
Vol 111 (1-2) ◽  
pp. 193-194 ◽  
Author(s):  
James A. Barnett ◽  
Anthony P. Sims
Keyword(s):  
Candida Utilis ◽  
Food Yeast ◽  
Kinetics Of Uptake ◽  
Kinetics Of

scholarly journals Influence of fluorophore and linker length on the localization and trafficking of fluorescent sterol probes

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jarmila Králová ◽  
Michal Jurášek ◽  
Lucie Mikšátková ◽  
Anna Marešová ◽  
Jan Fähnrich ◽  
...  
Keyword(s):  
Genetic Mutation ◽  
Cell Permeability ◽  
Specific Cell ◽  
Cell Compartments ◽  
Intracellular Compartments ◽  
Resolution Imaging ◽  
Intracellular Labelling ◽  
Kinetics Of Uptake ◽  
Kinetics Of ◽  
Derivatives Of

AbstractFluorescent sterol probes, comprising a fluorophore connected to a sterol backbone by means of a linker, are promising tools for enabling high-resolution imaging of intracellular cholesterol. In this study, we evaluated how the size of the linker, site of its attachment and nature of the fluorophore, affect the localization and trafficking properties of fluorescent sterol probes. Varying lengths of linker using the same fluorophore affected cell penetration and retention in specific cell compartments. A C-4 linker was confirmed as optimal. Derivatives of heterocyclic sterol precursors attached with identical C-4 linker to different fluorophores at diverse positions also showed significant differences in their binding properties to various intracellular compartments and kinetics of trafficking. Two novel red-emitting probes with good cell permeability, fast intracellular labelling and slightly different distribution displayed very promising characteristics for sterol probes. These probes also strongly labelled endo/lysosomal compartment in cells with pharmacologically disrupted cholesterol transport, or with a genetic mutation of cholesterol transporting protein NPC1, that overlapped with filipin staining of cholesterol. Overall, the present study demonstrates that the physicochemical properties of the fluorophore/linker pairing determine the kinetics of uptake and distribution and subsequently influence the applicability of final probes.

Kinetics of uptake and deacetylation of N-acetylcysteine by human erythrocytes

2007 ◽  
Vol 39 (9) ◽  
pp. 1698-1706 ◽  
Author(s):  
Julia E. Raftos ◽  
Stephney Whillier ◽  
Bogdan E. Chapman ◽  
Philip W. Kuchel
Keyword(s):  
Human Erythrocytes ◽  
Kinetics Of Uptake ◽  
Kinetics Of

scholarly journals Genome and Transcriptome Analysis of the Food-Yeast Candida utilis

PLoS ONE ◽  
2012 ◽  
Vol 7 (5) ◽  
pp. e37226 ◽  
Author(s):  
Yasuyuki Tomita ◽  
Kazuho Ikeo ◽  
Hideyuki Tamakawa ◽  
Takashi Gojobori ◽  
Shigehito Ikushima
Keyword(s):  
Transcriptome Analysis ◽  
Candida Utilis ◽  
Food Yeast

Sodium Exchange in the Frog Heart Ventricle

1957 ◽  
Vol 191 (3) ◽  
pp. 487-492 ◽  
Author(s):  
John A. Johnson
Keyword(s):  
Extracellular Space ◽  
Cell Membranes ◽  
Frog Heart ◽  
Heart Ventricle ◽  
Radioactive Sodium ◽  
Kinetics Of Uptake ◽  
Kinetics Of ◽  
Uptake And Release ◽  
Sodium Exchange

The isolated frog heart ventricle was used to study the kinetics of uptake and release of radioactive sodium. Sucrose was used as an extracellular space indicator. A flux of sodium across the ventricle cell membranes of 15 x 10–12 m/cm2 sec. was calculated from the data.

Production of lycopene by the food yeast,Candida utilis that does not naturally synthesize carotenoid

1998 ◽  
Vol 58 (2-3) ◽  
pp. 306-308 ◽  
Author(s):  
Yutaka Miura ◽  
Keiji Kondo ◽  
Hiroshi Shimada ◽  
Toshiko Saito ◽  
Katsumi Nakamura ◽  
...  
Keyword(s):  
Candida Utilis ◽  
Food Yeast
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