Endogenous growth regulator content in suspension cells of different male and female genotypes of asparagus

1993 ◽  
Vol 33 (3) ◽  
pp. 281-285
Author(s):  
Bruno Campion ◽  
Gabriella de Filippo ◽  
Elisabetta Caporali ◽  
Gianfranca Rossi ◽  
Giovanna Marziani
Keyword(s):  
Endogenous Growth ◽  
Growth Regulator ◽  
Suspension Cells ◽  
Male And Female ◽  
Endogenous Growth Regulator

scholarly journals Influence of low temperature on germination and endogenous growth regulator contents in Scots Pine (Pinus silvestris L.) seeds

2015 ◽  
Vol 42 (2) ◽  
pp. 233-240 ◽  
Author(s):  
J. Kopcewicz ◽  
Z. Poraziński
Keyword(s):  
Low Temperature ◽  
Endogenous Growth ◽  
Scots Pine ◽  
Growth Regulator ◽  
White Light ◽  
Simultaneous Increase ◽  
Pinus Silvestris ◽  
Endogenous Growth Regulator

Twenty days-long stratification of Scots pine seeds results in the seeds germinating in similar high percentage in darkness as in light. The period of stratification enough to overcome the "barrier" of light is connected with simultaneous increase of gibberellin contents, especially with the appearance of the new groups of gibberellins qualitatively similar to those occurring in pine tissues under the influence of red and white light. It was also found that seedlings grown from stratificated pine seeds produce the increased amounts of gibberellins.

Transient β-glucuronidase expression in Scots pine tissues derived from mature trees

1994 ◽  
Vol 24 (10) ◽  
pp. 2006-2011 ◽  
Author(s):  
Tuija Aronen ◽  
Hely Häggman ◽  
Anja Hohtola
Keyword(s):  
Transient Expression ◽  
Scots Pine ◽  
Growth Regulator ◽  
Genetic Regulation ◽  
Gus Expression ◽  
Expression Level ◽  
Suspension Cells ◽  
Mature Trees ◽  
Gene Construct ◽  
Bud Growth

Vegetative buds and bud-derived calli and suspension cells from 5- to 50-year-old Scots pines (Pinussylvestris L.) were used as targets for biolistic transformation. The gene construct used in the experiments was 35S CaMV–β-glucuronidase (GUS). The highest average level of transient GUS expression was found in suspension cells: 1229 ± 359 (mean ± SE) expressing cells per million. Transient expression was found in 35 of 44 (79%) tree genotypes studied. The expression level in buds and in calli was low: one or two spots per expressing bud. Growth-regulator pretreatment (BAP and 2,4-D) increased the number of GUS-expressing buds significantly. The high transient expression level in suspensions and the high percentage of GUS-expressing genotypes suggest that mature Scots pine tissues have potential for further transformation and genetic regulation studies.

scholarly journals Endogenous growth regulators in embryonic shoots of Scots pine at the time of male and female flower primordia initiation

2015 ◽  
Vol 46 (1) ◽  
pp. 119-128
Author(s):  
J. Kopcewicz ◽  
Z. Zatorska ◽  
H. Kulikowska ◽  
T. Szcześniak
Keyword(s):  
Abscisic Acid ◽  
Endogenous Growth ◽  
Scots Pine ◽  
Growth Regulators ◽  
Male Flower ◽  
Female Flower ◽  
Flower Primordia ◽  
Male And Female ◽  
Low Level

The initiation of Scots pine male flower primordia is connected with a high content of gibberellins and a low level of auxins, whereas the initiation of female flower primordia is correlated with a high content of auxins and a low level of gibberellins. There is lack of direct correlation between the content of cytokinins or abscisic acid and flower sex differentation.

Roles of endogenous and exogenous growth regulators in dormancy of Utricularia vulgaris

1979 ◽  
Vol 57 (24) ◽  
pp. 2750-2759 ◽  
Author(s):  
Robert D. Winston ◽  
Paul R. Gorham
Keyword(s):  
Abscisic Acid ◽  
Growth Regulators ◽  
Growth Regulator ◽  
Environmental Conditions ◽  
Vegetative Growth ◽  
Developmental Stages ◽  
Soybean Callus ◽  
Exogenous Growth ◽  
Endogenous Growth Regulator ◽  
Utricularia Vulgaris

The roles of endogenous and exogenous growth regulators on dormancy control of Utricularia vulgaris were investigated by extraction and bioassay of endogenous levels of four classes at various developmental stages and by measuring the effects of synthetic regulators on these stages. The bioassays used were oat coleoptile for auxins, abscisic acid and other inhibitors; lettuce hypocotyl for gibberellins; and soybean callus for cytokinins. Two weeks before turion formation and during innate dormancy abscisic acidlike and bound gibberellinlike levels were high while free gibberellinlike and auxinlike levels were low. Abscisic acid exogenously applied to actively growing plants rapidly induced turion formation even under noninductive environmental conditions. Kinetin rapidly and completely caused sprouting in innately dormant turions but would not promote continued vegetative growth. These plants formed new turions within 2 weeks of sprouting. During imposed dormancy, when turions rapidly sprouted, there was a low level of abscisic acidlike inhibitor and high levels of gibberellinlike and auxinlike compounds. Turions collected during this state could be maintained in a dormant condition by incubation with abscisic acid even at high temperatures which normally induce sprouting. During sprouting the endogenous growth regulator pattern was similar to that described for imposed dormancy. A model of the environmental and growth regulator factors which control dormancy is presented.

The Clinical, Roentgenological and Morphological Effects of an Acute Exposure of Phosgene on the Lungs of Dogs

1971 ◽  
Vol 29 ◽  
pp. 236-237
Author(s):  
R. F. Bils ◽  
W. F. Diller ◽  
F. Huth
Keyword(s):  
Latent Period ◽  
Chemical Industry ◽  
Toxic Substance ◽  
Lung Edema ◽  
X Rays ◽  
Beagle Dogs ◽  
Male And Female ◽  
Morphological Alterations ◽  
Before And After ◽  
Electron Microscopes

Phosgene still plays an important role as a toxic substance in the chemical industry. Thiess (1968) recently reported observations on numerous cases of phosgene poisoning. A serious difficulty in the clinical handling of phosgene poisoning cases is a relatively long latent period, up to 12 hours, with no obvious signs of severity. At about 12 hours heavy lung edema appears suddenly, however changes can be seen in routine X-rays taken after only a few hours' exposure (Diller et al., 1969). This study was undertaken to correlate these early changes seen by the roengenologist with morphological alterations in the lungs seen in the'light and electron microscopes.Forty-two adult male and female Beagle dogs were selected for these exposure experiments. Treated animals were exposed to 94.5-107-5 ppm phosgene for 10 min. in a 15 m3 chamber. Roentgenograms were made of the thorax of each animal before and after exposure, up to 24 hrs.

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