scholarly journals Effects of fungicides and bactericides on orchid seed germination and shoot tip cultures in vitro

1981 ◽  
Vol 1 (1) ◽  
pp. 165-180 ◽  
Author(s):  
D. M. Brown ◽  
C. L. Groom ◽  
M. Cvitanik ◽  
M. Brown ◽  
J. L. Cooper ◽  
...  
Keyword(s):  
Seed Germination ◽  
Shoot Tip ◽  
Orchid Seed ◽  
Orchid Seed Germination

A classroom exercise in hand pollination and in vitro asymbiotic orchid seed germination

2008 ◽  
Vol 93 (2) ◽  
pp. 223-230 ◽  
Author(s):  
Philip J. Kauth ◽  
Timothy R. Johnson ◽  
Scott L. Stewart ◽  
Michael E. Kane
Keyword(s):  
Seed Germination ◽  
Hand Pollination ◽  
Classroom Exercise ◽  
Orchid Seed ◽  
Orchid Seed Germination

scholarly journals In vitro Propagation and Forest Reestablishment of Cymbidium finlaysonianum Lindl., an Endangered Medicinal Orchid

2017 ◽  
Vol 15 (10) ◽  
pp. 711-724
Author(s):  
Suphat RITTIRAT ◽  
Sutha KLAOCHEED ◽  
Kanchit THAMMASIRI ◽  
Somporn PRASERTSONGSKUN
Keyword(s):  
Seed Germination ◽  
In Vitro Propagation ◽  
Germination Percentage ◽  
In Vitro Plant Regeneration ◽  
Embryo Viability ◽  
Viability Test ◽  
Orchid Seed ◽  
Medicinal Orchid ◽  
Orchid Seed Germination

An in vitro plant regeneration protocol was successfully established for Cymbidium finlaysonianum Lindl., an epiphytic endangered medicinal orchid, by culturing seeds from 4-month-old green self-pollinated fruits. Tetrazolium (TZ) viability test indicated a mean embryo viability of 97.80 %. Three asymbiotic orchid seed germination media, namely Knudson C medium (KC), New Dogashima medium (ND), and Vacin and Went medium (VW), were studied to select a suitable medium for seed germination. The highest seed germination percentage was 83.8 % on VW medium. Protocorm-like bodies (PLBs) of C. finlaysonianum Lindl. induced from protocorm segments cultured in VW liquid medium supplemented with 8.84 µM 6-benzylaminopurine (BAP) gave the highest % PLB formation and number of PLBs per explant, at 85.7 and 5.2, respectively. VW medium supplemented with 0.2 % (w/v) activated charcoal (AC) gave the highest number of roots per plantlet and root length, at 6.4 roots and 5.2 cm, respectively. The highest percentage of plantlet survival recorded at 24 months after reestablishment in the forest was 71.6 %.

scholarly journals Seed germination evaluation of Phalaenopsis amabilis in various media for long-term conservation

2021 ◽  
Vol 22 (11) ◽  
Author(s):  
DWI MURTI PUSPITANINGTYAS ◽  
ELIZABETH HANDINI
Keyword(s):  
Regression Analysis ◽  
Seed Germination ◽  
Life Span ◽  
Seed Storage ◽  
Optimum Conditions ◽  
Presidential Decree ◽  
Orchid Seed ◽  
Leaf Fertilizer

Abstract. Puspitaningtyas DM, Handini E. 2021. Seed germination evaluation of Phalaenopsis amabilis in various media for long-term conservation. Biodiversitas 22: 5231-5238. Phalaenopsis amabilis (L.) Blume (moon orchid) is one of Indonesia's national flowers, which is mentioned in Presidential Decree No. 4/1993. Study on the orchid seed of P. amabilis was conducted to observe the longevity of seeds storage. The purpose of this study was to determine the viability of P. amabilis seeds after storage for years, then to predict the life span of the seeds, and to find out the best media germination for P. amabilis seeds. After harvesting, P. amabilis seeds were stored at –20 °C and the germination tests were carried out periodically in different periods until the seeds lose their viability to determine the optimum conditions for long-term seed storage. Four trial media cultures have been used to test seed germination by means of in vitro culture. The result showed that seeds of P. amabilis were able to germinate in 4 media i.e. Knudson C, modified Knudson C, modified Vacin & Went and modified leaf fertilizer. The best germination was on leaf fertilizer medium. Based on the research showed that the viability seeds of P. amabilis decreased sharply after 6 years stored. By using regression analysis, the life span of the seeds could be predicted can reach 9 years of storage which is sowing on the best media (leaf fertilizer).

scholarly journals Studies on the Media for Orchid Seed Germination

1979 ◽  
Vol 48 (3) ◽  
pp. 345-352 ◽  
Author(s):  
Syoichi ICHIHASHI
Keyword(s):  
Seed Germination ◽  
Orchid Seed ◽  
The Media ◽  
Orchid Seed Germination

scholarly journals Studies on the Media for Orchid Seed Germination

1979 ◽  
Vol 47 (4) ◽  
pp. 524-536 ◽  
Author(s):  
Shoichi ICHIHASHI
Keyword(s):  
Seed Germination ◽  
Orchid Seed ◽  
The Media ◽  
Orchid Seed Germination

scholarly journals Asymbiotic seed germination and multiplication of an endangered orchid – Paphiopedilum venustum (Wall. ex Sims.)

2016 ◽  
Vol 85 (2) ◽  
Author(s):  
Saranjeet Kaur ◽  
Kamlesh Kumar Bhutani
Keyword(s):  
Seed Germination ◽  
Shoot Multiplication ◽  
Ex Situ ◽  
Terrestrial Orchid ◽  
Asymbiotic Seed Germination ◽  
Orchid Seed ◽  
Early Seedling ◽  
Potato Powder ◽  
Survival Frequency

The present study was intended to facilitate ex situ conservation of <em>Paphiopedilum venustum</em>, a highly floriferous endangered terrestrial orchid species. A protocol was established for in vitro propagation and shoot multiplication. The cultures were initiated through asymbiotic seed germination technique, using undehisced and dehisced capsules. Four defined asymbiotic orchid seed germination media (terrestrial orchid medium, modified terrestrial orchid medium, Malmgren modified terrestrial orchid medium, Knudson C medium) were evaluated for their effectiveness in achieving maximum seed germination and early seedling development. The effect of darkness and 12-h photoperiod was also tested. Optimum seed germination, i.e., 82.7% was achieved on modified terrestrial orchid medium under a 12-h photoperiod using seeds from undehisced capsules. Shoot multiplication was accomplished using organic [peptone (1.0, 2.0 g L<sup>−1</sup>)] and inorganic [banana homogenate (10, 20, 30 g L<sup>−1</sup>) and potato powder (5.0, 10 g L<sup>−1</sup>)] growth supplements. Peptone at 1.0 g L<sup>−1</sup> was the most effective in multiplying the shoots. Plantlets were acclimatized in the greenhouse with 80% survival frequency.

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